Research on organizational culture and teamwork is progressing substantially, and the interest in understanding culture as an organizational phenomenon and its links with other variables of organizations are perspectives that need to be considered to obtain a better understanding and knowledge of achieving organizational aims. The objective of this study was to analyze the research and its contributions to organizational culture and teamwork through indicators of quantity, quality and structure. The information was obtained from the Scopus and Web of Science databases through structured search equations. The bibliographic data were merged and processed using Microsoft Excel, and by applying and using the PRISMA 2020 statement, 836 records were obtained. VOSviewer software was used to map networks, overlay displays and analyze keyword densities. The findings revealed that organizational culture and teamwork are constantly expanding and being researched in different organizations, with studies relating organizational culture and teamwork to knowledge management, patient safety culture, knowledge exchange, interprofessional collaboration and innovation. The maximum number of publications on the topic occurred in 2019. As indicated by VOSviewer, the keyword communication was associated with three of the identified clusters, and the research agenda proposed the expanding of themes related to leadership, knowledge exchange, knowledge management, interprofessional collaboration and safety climate.