Organisation of Autonomic Nervous Structures in the Small Intestine of Chinchilla (<i>Chinchilla laniger</i>, Molina)

Nowak, E.

doi:10.1111/ahe.12077

Cited by 2 publications

(1 citation statement)

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“…Chinchillas were previously reared for their valuable fur, but in recent years this animal has more frequently served as a model species for research into human diseases, especially hearing dysfunction in addition to research related to digestive system and pneumonia [21]. They are also used as laboratory animals for studies of cerebral vascularization [22], innervation of the gastrointestinal system [23][24][25], and previously unreported morphology of the extrahepatic biliary tract [26].…”

Section: Introductionmentioning

confidence: 99%

Cholinergic and adrenergic innervation of the pancreas in chinchilla (Chinchilla Laniger Molina)

Radzimirska

Kuchinka

Nowak

et al. 2020

Folia Histochem Cytobiol.

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Introduction. Cholinergic and adrenergic innervation of the pancreas in chinchilla (Chinchilla Laniger Molina) was examined in this study. The pancreas is both an exocrine and endocrine gland with autonomic and sensory innervation presented by the numerous nerve fibers and small agglomerations of nerve cells. Material and methods. Investigations were performed on 16 adult chinchillas of both sexes. The material was collected immediately after death of the animals. Histochemical methods: AChE and SPG were used, in addition to routine technique of single and double immunohistochemical (IHC) staining using whole mount specimens and freezing sections with a thickness of 8 to 12 μm. In the immunofluorescence staining, primary antibodies directed against markers used to identify cholinergic -ChAT and VAChT, and adrenergic -DbH and TH neurons. Secondary antibodies were coupled to Alexa Fluor 488 and Alexa Fluor 555 fluorophores. Results. Histochemical studies (AChE) revealed that chinchilla pancreatic cholinergic innervation consisted of ganglionic neurocytes and numerous nerve fibers. These structures are located in the parenchyma of the exocrine part of the organ in close proximity to blood vessels and are present within the walls of the pancreatic ducts and interstitial connective tissue. A delicate fiber network around the Langerhans islets was also observed. The most numerous cholinergic structures were found in the head and tail, and the least numbers were found in the body of the pancreas. The SPG method revealed that adrenergic fibers form a network in the adventitia of blood vessels, and individual fibers run throughout the pancreatic parenchyma. Moreover, adrenergic nerve fibers were observed around the ganglionic neurocytes. This innervation was similar in all parts of the investigated organ. IHC investigations allowed observations of both the cholinergic and adrenergic activities of autonomic nerve structures. Additionally, using ChAT/DbH double staining, colocalization of these substances was observed in the fibers of the pancreatic parenchyma that passed through the cholinergic ganglia. Colocalization of VAChT and TH was found in nerve fibers of the exocrine part, in the walls of blood vessels, and in individual nerve cells. Colocalization of ChAT/DbH and VAChT/TH was observed in the single nerve cells and in the small (2-3 cell) ganglia. ChAT-and DbH-immunopositive nerve fibers were found in the area of the islets of Langerhans. Conclusions.The results indicate a more intense cholinergic innervation of the chinchilla's pancreas, which is represented by both ganglia and nerve fibers, while adrenergic structures are mainly represented by fibers and only single neurocytes. This arrangement of the investigated structures in this species may imply a major role for hormonal control of exocrine secretion in rodents.

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Section: Introductionmentioning

confidence: 99%

Cholinergic and adrenergic innervation of the pancreas in chinchilla (Chinchilla Laniger Molina)

Radzimirska

Kuchinka

Nowak

et al. 2020

Folia Histochem Cytobiol.

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Neuroendocrine structures of the small intestine of the capybara Hydrochoerus hydrochaeris (Mammalia, Rodentia)

et al. 2018

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A complex network of nerve fibers of the enteric nervous system and enteroendocrine cells is known to regulate the gastrointestinal tract. The distribution and frequency of the argyrophil, argentaffin and serotonin immunoreactive endocrine cells and of the submucosal and myenteric nervous ganglia were studied in the small intestine of the capybara Hydrochoerus hydrochaeris, aiming to verify the existence of possible numerical correlations between endocrine cells and nervous ganglia. Fragments of the duodenum, jejunum and ileum of adult animals were collected and processed according to routine histological techniques. To study the nervous ganglia, hematoxylin and eosin staining was used, while specific staining techniques were used to study the argyrophil, argentaffin and serotonin immunoreactive endocrine cells: Grimelius, modified Masson-Fontana and peroxidase anti-peroxidase, respectively. Endocrine cells were more abundant in the area of the crypts and, in relation to their morphology, ‘open type’ endocrine cells prevailed. The population of argyrophil cells was larger than that of argentaffin cells, and these cells were larger than serotonin immunoreactive cells. The frequency of endocrine cells was apparently greater in the duodenum, indicating the importance of this intestinal segment in digestive and absorptive functions. Prominent nervous ganglia were observed in the submucosal and myenteric plexi, and were larger and more frequent in the myenteric plexus. A numerical correlation was found among the endocrine cells (argentaffin and serotonin immunoreactive cells) and the myenteric nervous ganglia, suggesting the presence of physiological interactions among the endocrine and nervous systems for the control of intestinal activities. The findings in this study contribute to the understanding of the digestive processes of this species, which may also help in its conservation and future survival.

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Organisation of Autonomic Nervous Structures in the Small Intestine of Chinchilla (Chinchilla laniger, Molina)

Cited by 2 publications

References 30 publications

Cholinergic and adrenergic innervation of the pancreas in chinchilla (Chinchilla Laniger Molina)

Cholinergic and adrenergic innervation of the pancreas in chinchilla (Chinchilla Laniger Molina)

Neuroendocrine structures of the small intestine of the capybara Hydrochoerus hydrochaeris (Mammalia, Rodentia)

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