Organic phase enzyme electrodes

Conducting polymers in chemical sensors and arrays

“…Few examples of biosensors prepared by this procedure were proposed for operation in non-aqueous solvents [166]: the enzyme was adsorbed on the solid support from aqueous phase and being insoluble in organics, it does not diffuse away from the support into the bulk solution [166].…”

Leveraging protein purification strategies in proteomics

“…As a large number of membranes for ultrafiltration [156], nanofiltration [158] and affinity membranes [159,160] are available, this may have a good potential in proteomics. From analytical perspective, enzyme analysis in nonaqueous media is more or less an unexplored territory [161,162]. There is a lot which is happening in the area like biosensors and protein refolding [163][164][165][166].…”

Organophosphorus Pesticides Analysis

“…It was demonstrated that artificial neural networks implementation could resolve mixtures of pesticides (Alonso et al, 2010). Exhaustive reviews on enzyme inhibition-based biosensors, including inhibition determination in organic phase are provided by Amine et al (2006) and López et al (2006). The application of various enzymes: acetylcholinesterase, acid phosphatase, alkaline phosphatise, organophosphorus hydrolase, and tyrosinase for the quantification of OP pesticides in the environment is extensively revised by Van Dyk et al (2011).…”