Organic Monolith Column Technology for Capillary Liquid Chromatography

Y, Li; Aggarwal, Pankaj; Hd, Tolley; Ml, Lee

doi:10.1201/b11636-6

Cited by 6 publications

(7 citation statements)

References 198 publications

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“…The increase in monomer concentration leads to the formation of more nuclei upon irradiation. As these nuclei pick up monomers and grow, they also attach to each other before they become too large, generating small voids (through-pores) in the microglobular monolithic structure . The same trend was observed from CFP measurements, i.e., the permeability and pore size decreased with an increase in the percentage of monomer.…”

Section: Resultssupporting

confidence: 73%

“…As these nuclei pick up monomers and grow, they also attach to each other before they become too large, generating small voids (through-pores) in the microglobular monolithic structure. 9 The same trend was observed from CFP measurements, i.e., the permeability and pore size decreased with an increase in the percentage of monomer. The crossover points of the wet and half-dry curves gave mean through-pore diameters of 1.64, 1.20, and 0.80 for the columns listed in Table 2.…”

Section: Capillary Flow Porometry (Cfp)supporting

confidence: 72%

“…The efficiencies (i.e., plate height, H) of monolithic columns have been reported to vary directly with domain size. 9 A decrease in domain size results in a reduced diffusion length for the analyte across the bed structure, thereby decreasing the contribution of the C term in the van Deemter equation and lowering the H value. A decrease in through-pore size reduces the resistance to mass transfer in the mobile phase, while a decrease in skeleton size decreases the resistance to mass transfer in the stationary phase.…”

Section: Capillary Flow Porometry (Cfp)mentioning

confidence: 99%

“…The advent of monolithic column technology offers the potential of providing both good efficiency and high permeability. However, monolithic column performance has not generally matched that of particle packed columns . This can be attributed to the heterogeneity of the monolithic skeletal structure and its wide through-pore size distribution.…”

mentioning

confidence: 99%

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Characterizing Organic Monolithic Columns Using Capillary Flow Porometry and Scanning Electron Microscopy

2011

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Polyethylene glycol diacrylate monoliths prepared using different amounts of monomer, porogen ratio, and capillary dimensions were characterized using capillary flow porometry (CFP) and scanning electron microscopy (SEM). Our results reveal good agreement between SEM and CFP measurements for through-pore size distribution. The CFP measurements for monoliths prepared by the same procedure in capillaries with different diameters (i.e., 75, 150, and 250 μm) clearly confirmed a change in through-pore size distribution with capillary diameter, thus, certifying the need for in-column measurement techniques over bulk measurements (e.g., mercury intrusion porosimetry). The mean through-pore size varied from 3.52 to 1.50 μm with a change in capillary diameter from 75 to 250 μm. Consistent mean through-pore size distribution for capillary columns with the same internal diameter but with different lengths (1.5, 2, and 3 cm) confirms the high interconnectivity of the pores and independence of CFP measurements with respect to capillary length. CFP and SEM measurements not only allow pore structure analysis but also prediction of relative column performance. Monoliths with narrow through-pore size distribution (0.8-1.2 μm), small mean through-pore size, and thin skeletal size (0.55 μm) gave the best performance in terms of efficiency for polyethylene glycol diacrylate monoliths.

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Section: Resultssupporting

confidence: 73%

Section: Capillary Flow Porometry (Cfp)supporting

confidence: 72%

Section: Capillary Flow Porometry (Cfp)mentioning

confidence: 99%

mentioning

confidence: 99%

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Characterizing Organic Monolithic Columns Using Capillary Flow Porometry and Scanning Electron Microscopy

2011

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“…The monoliths can be synthesized separately and then cladded with a pressure-resistant column wall material although this manufacturing process is demanding. However, monomers can also easily be synthesized within the column and most interestingly even within capillaries (Li et al, 2012). Suitable monomers are, for example, alkyl dimethacrylates (such as dodecanediol dimethacrylate) dissolved in methanol-dodecanol mixture that contains an initiator.…”

Section: Stationary Phasesmentioning

confidence: 99%

High Pressure Liquid Chromatography and Ultra‐high Pressure Liquid Chromatography of Plants: Basic Concepts

Meyer

2014

Encyclopedia of Analytical Chemistry

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High‐performance liquid chromatography (HPLC) is an essential method in plant analysis. Although it is primarily a separation method, it offers also the possibility of on‐line quantitative analysis during the separation. It can be used for both, the investigation of classes of compounds as well as of individual substances. HPLC is an instrumental method of great flexibility with regard to the separation conditions and to the detection principle. In any case, high pressure is needed; conventional HPLC acts up to about 350 bar. As the available pressure limits the speed of analysis and its separation power, the more recent technology of ultra high‐performance liquid chromatography (UHPLC) works at pressures up to 1000 bar. It allows very fast analyses or high peak capacity (i.e., the number of resolved compounds) or a combination of both demands. The separation environment can be aqueous, for example, in reversed‐phase or ion‐exchange HPLC, or nonpolar with organic solvents in adsorption chromatography, thus allowing a great choice of investigative principles.

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Theory and Practice of Two‐Dimensional Liquid Chromatography Separations Involving the HILIC Mode of Separation

Groskreutz

Stoll

2013

Hydrophilic Interaction Chromatography

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Organic Monolith Column Technology for Capillary Liquid Chromatography

Cited by 6 publications

References 198 publications

Characterizing Organic Monolithic Columns Using Capillary Flow Porometry and Scanning Electron Microscopy

Characterizing Organic Monolithic Columns Using Capillary Flow Porometry and Scanning Electron Microscopy

High Pressure Liquid Chromatography and Ultra‐high Pressure Liquid Chromatography of Plants: Basic Concepts

Theory and Practice of Two‐Dimensional Liquid Chromatography Separations Involving the HILIC Mode of Separation

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