Organic–inorganic hybrid nanoflowers: A novel host platform for immobilizing biomolecules

Cui, Jiandong; Jia, Shiru

doi:10.1016/j.ccr.2017.09.008

Cited by 201 publications

(99 citation statements)

References 134 publications

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“…Further, this kinetically controlled process gives rise to separate petals . Although the method of incubation leads to the formation of hybrid nanoassemblies but the most conspicuous downside of the process is long reaction time of 72 h. Another striking feature hindering the practical applications of these nanohybrids is poor recyclability attributed mainly to friability of the hierarchically shaped petals during centrifugation steps of recycling process . Therefore, there is a significant need for the design of a less time consuming method for synthesizing enzyme‐inorganic hybrids with simultaneous enhancement of robustness and recyclability.…”

Section: Introductionmentioning

confidence: 99%

Self Assembly through Sonication: An Expeditious and Green Approach for the Synthesis of Organic‐Inorganic Hybrid Nanopetals and their Application as Biocatalyst

et al. 2018

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The present study exhibits a facile method for the expeditious synthesis of organic-inorganic hybrid nanoassemblies. Pseudomonas fluorescens lipase was immobilized on the nanoassembly and its application as a nanobiocatalyst was demonstrated. Scanning electron microscopy analysis depicted petal-like morphology of the newly synthesized hybrid. The sonochemical method screened as a faster route for the synthesis of hybrid nanopetals with a marked increase in enzyme activity (15 folds). Furthermore, an additional treat-ment of the hybrid nanopetals with chemical modifiers likes 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) exhibited improved stability and reusability upto six cycles. A systematic investigation of the reaction parameters influencing the formation of lipase-embedded hybrid nanopetals was done. In addition, the nanobiocatalytic potential of hybrid nanopetals was explored in the kinetic resolution of racemic secondary alcohols.

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Section: Introductionmentioning

confidence: 99%

Self Assembly through Sonication: An Expeditious and Green Approach for the Synthesis of Organic‐Inorganic Hybrid Nanopetals and their Application as Biocatalyst

et al. 2018

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“…Additionally, the synthesis of ZnO nanomaterials is convenient, easy, hassle-free, economical, and eco-friendly, and thus it proves to be more favorable in cases of clinical detection [56]. Some studies have demonstrated that ZnO can bind to the enzymes to enhance the enzyme activity [57][58][59][60]. Scientists and researchers have been interested in nanoflowers, a relatively newer type of nanostructure, due to the positional characteristics of nanolayers.…”

Section: Introductionmentioning

confidence: 99%

“…Scientists and researchers have been interested in nanoflowers, a relatively newer type of nanostructure, due to the positional characteristics of nanolayers. These nanolayers have a special area, that facilitates a higher surface-to-volume ratio when compared to classic spherical nanoparticles, which in turn drastically shoot up the efficiency of surface reactions for nanoflowers [60]. Development of a ZnO nanoflower or flower-like ZnO-assisted nanoPCR for the efficient and quick diagnosis of canine vector-borne diseases (CVBDs) has rarely been reported [61].…”

Section: Introductionmentioning

confidence: 99%

ZnO Nanoflower-Based NanoPCR as an Efficient Diagnostic Tool for Quick Diagnosis of Canine Vector-Borne Pathogens

Upadhyay

Yang²,

Zaman

et al. 2020

Pathogens

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Polymerase chain reaction (PCR) is a unique technique in molecular biology and biotechnology for amplifying target DNA strands, and is also considered as a gold standard for the diagnosis of many canine diseases as well as many other infectious diseases. However, PCR still faces many challenges and issues related to its sensitivity, specificity, efficiency, and turnaround time. To address these issues, we described the use of unique ZnO nanoflowers in PCR reaction and an efficient ZnO nanoflower-based PCR (nanoPCR) for the molecular diagnosis of canine vector-borne diseases (CVBDs). A total of 1 mM of an aqueous solution of ZnO nanoflowers incorporated in PCR showed a significant enhancement of the PCR assay with respect to its sensitivity and specificity for the diagnosis of two important CVBDs, Babesia canis vogeli and Hepatozoon canis. Interestingly, it drastically reduced the turnaround time of the PCR assay without compromising the yield of the amplified DNA, which can be of benefit for veterinary practitioners for the improved management of diseases. This can be attributed to the favorable adsorption of ZnO nanoflowers to the DNA and thermal conductivity of ZnO nanoflowers. The unique ZnO nanoflower-assisted nanoPCR greatly improved the yield, purity, and quality of the amplified products, but the mechanism behind these properties and the effects and changes due to the different concentrations of ZnO nanoflowers in the PCR system needs to be further studied.

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“…Currently, the inorganic metal ions used to prepare hNFs have also been expanded from Cu 2+ to Zn 2+ , Co 2+ , Ca 2+ , Mn 2+ , and so forth. [25][26][27][28]. Further, biomimetic mineralization has been developed as a convenient method to prepare biomimetic materials [29,30] such as artificial bone.…”

Section: Introductionmentioning

confidence: 99%

Facile Bioinspired Preparation of Fluorinase@Fluoridated Hydroxyapatite Nanoflowers for the Biosynthesis of 5′-Fluorodeoxy Adenosine

Wang

et al. 2020

Sustainability

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To develop an environmentally friendly biocatalyst for the efficient synthesis of organofluorine compounds, we prepared the enzyme@fluoridated hydroxyapatite nanoflowers (FHAp-NFs) using fluorinase expressed in Escherichia coli Rosetta (DE3) as the biomineralization framework. The obtained fluorinase@FHAp-NFs were characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), and FT-IR spectrum and used in the enzymatic synthesis of 5′-fluorodeoxy adenosin with S-adenosyl-L-methionine and fluoride as substrate. At an optimum pH of 7.5, fluorinase confined in the hybrid nanoflowers presents an approximately 2-fold higher synthetic activity than free fluorinase. Additionally, after heating at 30 °C for 8 h, the FHAp-NFs retained approximately 80.0% of the initial activity. However, free enzyme could remain only 48.2% of its initial activity. The results indicate that the fluoride and hybrid nanoflowers efficiently enhance the catalytic activity and thermal stability of fluorinase in the synthesis of 5′-fluorodeoxy adenosine, which gives a green method for producing the fluorinated organic compounds.

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Organic–inorganic hybrid nanoflowers: A novel host platform for immobilizing biomolecules

Cited by 201 publications

References 134 publications

Self Assembly through Sonication: An Expeditious and Green Approach for the Synthesis of Organic‐Inorganic Hybrid Nanopetals and their Application as Biocatalyst

Self Assembly through Sonication: An Expeditious and Green Approach for the Synthesis of Organic‐Inorganic Hybrid Nanopetals and their Application as Biocatalyst

ZnO Nanoflower-Based NanoPCR as an Efficient Diagnostic Tool for Quick Diagnosis of Canine Vector-Borne Pathogens

Facile Bioinspired Preparation of Fluorinase@Fluoridated Hydroxyapatite Nanoflowers for the Biosynthesis of 5′-Fluorodeoxy Adenosine

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