Organic-Inorganic Hybrid Nanoflower Production and Analytical Utilization: Fundamental to Cutting-Edge Technologies

Subramani, Indra Gandi; Perumal, Veeradasan; Gopinath, Subash C. B.; Fhan, Khor Shing; Mohamed, Norani Muti

doi:10.1080/10408347.2021.1889962

Cited by 20 publications

(10 citation statements)

References 121 publications

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“…In subsequent studies summarized in comprehensive review articles, different metal ions, for instance, Ca 2+ , Zn 2+ , Mn 2+ , Mg 2+ , Fe 2+ , Co 2+ , etc. and numerous single enzymes have been used to design NF-based biocatalysts and biosensors applicable in biotechnology, biomedicine, and environmental sciences [ 30 , 31 , 32 , 33 , 34 ]. Since a nitrogen atom is involved in the complexation during the NF formation [ 29 , 30 ], besides enzymes and other nitrogen-containing biomolecules such as aptamers, natural DNA, amino acids, and chitosan, elastin-like polypeptides have also been used as the organic element to construct self-assembled NF [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

“…and numerous single enzymes have been used to design NF-based biocatalysts and biosensors applicable in biotechnology, biomedicine, and environmental sciences [ 30 , 31 , 32 , 33 , 34 ]. Since a nitrogen atom is involved in the complexation during the NF formation [ 29 , 30 ], besides enzymes and other nitrogen-containing biomolecules such as aptamers, natural DNA, amino acids, and chitosan, elastin-like polypeptides have also been used as the organic element to construct self-assembled NF [ 34 ]. Multienzyme NF assemblies capable to catalyze cascade reactions have also been developed.…”

Section: Introductionmentioning

confidence: 99%

“…In spite of their undisputed qualities as efficient biocatalysts, hybrid NF have shown some drawbacks that impede their wide industrial application. Due to multiple centrifugation/filtration cycles during the NF collection [ 31 , 32 , 33 , 34 ], or in extremely acidic media [ 37 ], the delicate hierarchically ordered petals are easily broken. Along with their mechanical fragility and sensitivity to pH, conventional NF particles are too small to be filtered off easily, cannot be reused after the first reaction cycle, and their synthesis could be difficult to scale up.…”

Section: Introductionmentioning

confidence: 99%

“…Along with their mechanical fragility and sensitivity to pH, conventional NF particles are too small to be filtered off easily, cannot be reused after the first reaction cycle, and their synthesis could be difficult to scale up. Consequently, there appears a demand for innovative, highly robust, and recyclable hybrid NF systems useful for enzyme-based applications such as bioremediation, biosensing, and biocatalysis [ 34 ]. In this context, chemical treatment and incorporation of other nano or microsized materials have been tried to make NF more efficient for industrial applications [ 38 , 39 , 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of Novel Polymer-Assisted Organic-Inorganic Hybrid Nanoflowers and Their Application in Cascade Biocatalysis

et al. 2023

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This study reports on the synthesis of novel bienzyme polymer-assisted nanoflower complexes (PANF), their morphological and structural characterization, and their effectiveness as cascade biocatalysts. First, highly porous polyamide 6 microparticles (PA6 MP) are synthesized by activated anionic polymerization in solution. Second, the PA6 MP are used as carriers for hybrid bienzyme assemblies comprising glucose oxidase (GOx) and horseradish peroxidase (HRP). Thus, four PANF complexes with different co-localization and compartmentalization of the two enzymes are prepared. In samples NF GH/PA and NF GH@PA, both enzymes are localized within the same hybrid flowerlike organic-inorganic nanostructures (NF), the difference being in the way the PA6 MP are assembled with NF. In samples NF G/PAiH and NF G@PAiH, only GOx is located in the NF, while HRP is preliminary immobilized on PA6 MP. The morphology and the structure of the four PANF complexes have been studied by microscopy, spectroscopy, and synchrotron X-ray techniques. The catalytic activity of the four PANF was assessed by a two-step cascade reaction of glucose oxidation. The PANF complexes are up to 2–3 times more active than the free GOx/HRP dyad. They also display enhanced kinetic parameters, superior thermal stability in the 40–60 °C range, optimum performance at pH 4–6, and excellent storage stability. All PANF complexes are active for up to 6 consecutive operational cycles.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synthesis of Novel Polymer-Assisted Organic-Inorganic Hybrid Nanoflowers and Their Application in Cascade Biocatalysis

et al. 2023

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“…Porous nanoparticles not only have a bigger surface, but in addition improve physicochemical properties. It has really been demonstrated that they are useful in lots of areas, including sensing, catalysis, separation, and storage applications (Subramani et al 2021 ). Nanoparticles have a wide range of use, because they are nano-sized, they provide volume advantages, they have a reactive structure and they have antibacterial properties (Martín-Moreno et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

A comparative study of iron nanoflower and nanocube in terms of antibacterial properties

et al. 2023

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It is known that heavy metal containing nanomaterials can easily prevent the formation of microbial cultures. The emergence of new generation epidemic diseases in the last 2 years has increased the importance of both personal and environmental hygiene. For this reason, in addition to preventing the spread of diseases, studies on alternative disinfectant substances are also carried out. In this study, the antibacterial activity of nanoflower and nanocube, which are easily synthesized and nanoparticle species containing iron, were compared. The antioxidant abilities of new synthesized NF@FeO(OH) and NC@α-Fe 2 O 3 were tested by DPPH scavenging activity assay. The highest DPPH inhibition was achieved with NC@α-Fe 2 O 3 as 71.30% at 200 mg/L. NF@FeO(OH) and NC@α-Fe 2 O 3 demonstrated excellent DNA cleavage ability. The antimicrobial capabilities of NF@FeO(OH) and NC@α-Fe 2 O 3 were analyzed with micro dilution procedure. In 500 mg/L, the antimicrobial activity was 100%. In addition to these, the biofilm inhibition of NF@FeO(OH) and NC@α-Fe 2 O 3 were investigated against S. aureus and P. aeruginosa and it was found that they showed significant antibiofilm inhibition. It is suggested that additional studies can be continued to be developed and used as an antibacterial according to the results of the nanoparticles after various toxicological test systems.

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Role of pretty nanoflowers as novel versatile analytical tools for sensing in biomedical and bioanalytical applications

Dadi,

Ocsoy

2024

Smart Medicine

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In recent years, an encouraging breakthrough in the synthesis of immobilized enzymes in flower‐shaped called “organic‐inorganic hybrid nanoflowers (hNFs)” with greatly enhanced catalytic activity and stability were reported. Although, these hNFs were discovered by accident, the enzymes exhibited highly enhanced catalytic activities and stabilities in the hNFs compared with the free and conventionally immobilized enzymes. Herein, we rationally utilized the catalytic activity of the hNFs for analytical applications. In this comprehensive review, we covered the design and use of the hNFs as novel versatile sensors for electrochemical, colorimetric/optical and immunosensors‐based detection strategies in analytical perspective.

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Organic-Inorganic Hybrid Nanoflower Production and Analytical Utilization: Fundamental to Cutting-Edge Technologies

Cited by 20 publications

References 121 publications

Synthesis of Novel Polymer-Assisted Organic-Inorganic Hybrid Nanoflowers and Their Application in Cascade Biocatalysis

Synthesis of Novel Polymer-Assisted Organic-Inorganic Hybrid Nanoflowers and Their Application in Cascade Biocatalysis

A comparative study of iron nanoflower and nanocube in terms of antibacterial properties

Role of pretty nanoflowers as novel versatile analytical tools for sensing in biomedical and bioanalytical applications

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