“…A. tumefaciens strain GV 3101, a nopaline strain, C58 type chromosome, carrying the binary vector plasmid system pBin -Xpat122 CAT, a modified Вin19 vector (provided by M. Bevan, AFRC, U. K.) and pGV3850 (provided by J. Schell, Max Planck Inst,, Koln) was used. pGV3850 is the helper plasmid, and pBin -Xpat122 contains the coding sequence of the bacterial neomycin phosphotransferase II (NPT II) gene under the control of the nopaline synthase promoter and the bacterial CAT gene under the control of the Xpatl22 patatin promoter class I, which was early obtained in our laboratory [11,15].…”