Organ-specific gene expression in transgenic potato: the cloning a new promoter of a class I patatin gene

Abstract

Organ-specific gene expression in transgenic potato: the cloning a new promoter of a class I patatin gene Using synthetic oligonucleotide probes homologous to conservative AT-rich motif of patatin genes class I of two differentclones were isolatedfrom a potato genomic library. One of two different genomic clones named X pat 122 was subcloned and analysis 5'-region sequences. Using the chloramphenicol acetyitransferase (CAT) gene as a reporter it has been shown that a L8 kb promoter fragment of the class I patatin gene PAT J 22 provides all the information necessary for both tuber-specific and sucrose-induced expression in leaves in transgenic potato plants.

Genetic transformation of potato (Solanum tuberosum L.) using a binary Agrobacterium tumefaciens vector with patatin promoter class I

“…A. tumefaciens strain GV 3101, a nopaline strain, C58 type chromosome, carrying the binary vector plasmid system pBin -Xpat122 CAT, a modified Вin19 vector (provided by M. Bevan, AFRC, U. K.) and pGV3850 (provided by J. Schell, Max Planck Inst,, Koln) was used. pGV3850 is the helper plasmid, and pBin -Xpat122 contains the coding sequence of the bacterial neomycin phosphotransferase II (NPT II) gene under the control of the nopaline synthase promoter and the bacterial CAT gene under the control of the Xpatl22 patatin promoter class I, which was early obtained in our laboratory [11,15].…”

“…The promoter of class I patatine genes is tuber-specific. Giving the important role of sucrose levels in determining gene expression, it is possible that the distribution of patatine in mature potato plants could be determined by the high concentration of sucrose [4,15]. The class II patatin genes encode only 1 -2 '% of the patatin mRNA in tubers, but unlike the class I genes, they are also normally expressed at low levels in roots.…”