Genetic transformation of potato (Solanum tuberosum L.) using a binary Agrobacterium tumefaciens vector with patatin promoter class I

Abstract

Kanamycin resistant plants of S. tuberosum L. (in vitro-grown) cv. Zarevo were obtained from the cocultivated microtubers with A. tumefaciens. A disarmed binary vector systems containing the neomycin phosphotransferase (NPT II) gene as selectable marker and chloramphenicol acetyltransferase (CAT), as a reporter gene, under control of new patatin promoter class I were utilized. In vitro grown minitubers discs were used as sources of explants to produce transgenic plants on selective medium containing 100 jug/1 kanamycin and CAT enzyme activities were detected.