Organ-specific dietary fatty acid uptake in humans using positron emission tomography coupled to computed tomography

Labbé, Sébastien M.; Grenier-Larouche, Thomas; Croteau, Étienne; Normand-Lauzière, François; Frisch, Frédérique; Ouellet, René; Guérin, Brigitte; Turcotte, Éric; Carpentier, André C.

doi:10.1152/ajpendo.00579.2010

Cited by 78 publications

(88 citation statements)

References 35 publications

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“…Excess exposure of lean tissues to fatty acids may stem from a disordered storage of dietary fatty acids (DFAs) in adipocytes (4)(5)(6)(7). We recently developed a novel method for noninvasive measurement of organ-specific DFA uptake and partitioning using 14(R,S)-[ (8). Using this method, we found that subjects with impaired glucose tolerance (IGT) displayed increased myocardial DFA partitioning and fractional uptake that correlated negatively with left ventricular ejection fraction (9).…”

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confidence: 99%

Effect of Sex and Impaired Glucose Tolerance on Organ-Specific Dietary Fatty Acid Metabolism in Humans

et al. 2014

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Oral 14(R,S)-[18 F]-fluoro-6-thia-heptadecanoic acid was used to determine whether an increase in cardiac dietary fatty acid (DFA) metabolism in impaired glucose tolerance (IGT) is different in men and women. Myocardial DFA partitioning after 6 h was higher in IGT versus control subjects (P = 0.006) in both men (2.14 [95% CI sex difference. Men had higher net myocardial DFA uptake between time 90 and 120 min driven by higher chylomicrontriglyceride (TG) levels. IGT-associated increased cardiac DFA partitioning was directly related to obesity in women, whereas it was associated with IGT per se in men. We conclude that early cardiac DFA uptake is higher in men driven by change in postprandial chylomicron-TG level but that increase in 6-h postprandial cardiac DFA partitioning nevertheless occurs with IGT both in men and women.Sex, obesity, and insulin resistance appear to act independently but also in concert on myocardial metabolism, structure, and function. Studies conducted in the fasting state show that women have greater myocardial oxygen consumption (1), which increases with obesity (2). In addition, female sex appears to be an important predictor of higher diabetes-associated cardiac plasma nonesterified fatty acid (NEFA) utilization (3). Excess exposure of lean tissues to fatty acids may stem from a disordered storage of dietary fatty acids (DFAs) in adipocytes (4-7). We recently developed a novel method for noninvasive measurement of organ-specific DFA uptake and partitioning using 14(R,S)-[ 8). Using this method, we found that subjects with impaired glucose tolerance (IGT) displayed increased myocardial DFA partitioning and fractional uptake that correlated negatively with left ventricular ejection fraction (9). Subjects with IGT also displayed reduced DFA partitioning in abdominal adipose tissues (9), in accordance with findings using conventional tracer methods (10-12).Due to the small sample size at the time of our previous report, it was not possible to assess whether any sexrelated differences in organ-specific DFA metabolism were present and could modulate the effect of IGT on cardiac metabolism. Based on previous reports on cardiac NEFA metabolism (3), the hypothesis of the current study was that elevated DFA uptake and partitioning observed with IGT would be worsened in women versus men. A secondary exploratory objective of our study was to determine sexrelated differences in liver, skeletal muscle, and adipose tissue DFA partitioning according to IGT status. RESEARCH DESIGN AND METHODS Study ParticipantsForty-one Caucasian individuals (see Table 1) with normal (IGT 2 ) and IGT (IGT + ), defined as having a 2-h post 75-g

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Effect of Sex and Impaired Glucose Tolerance on Organ-Specific Dietary Fatty Acid Metabolism in Humans

et al. 2014

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“…Each participant underwent a 6-h postprandial experimental protocol after both the 7-day LOWCAL and ISOCAL diets, in which a standard liquid meal prepared as described (6) Each subject was placed in the supine position and scanned using a 16-slice PET/computed tomography (CT) scanner (Gemini TF; Philips, Eindhoven, the Netherlands) (13). At time 260 min before meal intake, [ and electrocardiogram gating was performed, allowing the application of PET ventriculography to determine cardiac function, as previously published (14,15 (16), was mixed into Intralipid 20% (Baxter, Mississauga, Ontario, Canada), incorporated into gel capsules (T U B Enterprises), and given orally with the liquid meal.…”

Section: Experimental Protocolsmentioning

confidence: 99%

“…At time 260 min before meal intake, [ and electrocardiogram gating was performed, allowing the application of PET ventriculography to determine cardiac function, as previously published (14,15 (16), was mixed into Intralipid 20% (Baxter, Mississauga, Ontario, Canada), incorporated into gel capsules (T U B Enterprises), and given orally with the liquid meal. A dynamic list-mode PET scan centered on the thoracoabdominal segment was performed between time 90 and 120 min, and a whole-body PET/CT acquisition at time 360 min was performed as previously described (13). The maximal gastrointestinal tract radioactivity exposure for oral [ 18 F]-FTHA administration was estimated at 2.35 mSv in the stomach.…”

Section: Experimental Protocolsmentioning

confidence: 99%

“…Chylomicrons and plasma lipids were separated by ultracentrifugation and assayed for [ 18 F] activity and TG concentration (13). The Matsuda index was determined as previously described (20).…”

Section: Laboratory Assays and Assessment Of Insulin Resistancementioning

confidence: 99%

“…In animal and human dietary trials, the replacement of TG-raising carbohydrates with monounsaturated or n3 polyunsaturated fats (12) improved cardiac function. Recently, using the noninvasive method of positron emission tomography (PET) imaging with the oral administration of 14(R,S)-[ acid analog (13), we have shown that subjects with impaired glucose tolerance (IGT) display an increase in myocardial DFA uptake and partitioning (defined as the relative biodistribution of DFA among all organs) that is associated with reduced left ventricular function (14). These metabolic and functional cardiac abnormalities were corrected after a 1-year lifestyle intervention with modest weight loss (15).…”

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Seven-Day Caloric and Saturated Fat Restriction Increases Myocardial Dietary Fatty Acid Partitioning in Impaired Glucose-Tolerant Subjects

et al. 2015

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Subjects with impaired glucose tolerance (IGT) have increased myocardial partitioning of dietary fatty acids (DFAs) with left ventricular dysfunction, both of which are improved by modest weight loss over 1 year induced by lifestyle changes. Here, we determined the effects of a 7-day hypocaloric diet (2500 kcal/day) low in saturated fat (<7% of energy) (LOWCAL study) versus isocaloric with the usual amount saturated fat (∼10% of energy) diet (ISOCAL) on DFA metabolism in subjects with IGT. Organ-specific DFA partitioning and cardiac and hepatic DFA fractional uptake rates were measured in 15 IGT subjects (7 males/8 females) using the oral 14(R,S)-[18 F]-fluoro-6-thia-heptadecanoic acid positron emission tomography method after 7 days of an ISOCAL diet versus a LOWCAL diet using a randomized crossover design. The LOWCAL diet led to reductions in weight and postprandial insulin area under the curve. Myocardial DFA partitioning over 6 h was increased after the LOWCAL diet (2.3 6 0.1 vs. 1.9 6 0.2 mean standard uptake value, P < 0.04). However, the early (90-120 min) myocardial DFA fractional uptake was unchanged after the LOWCAL diet (0.055 6 0.025 vs. 0.046 6 0.009 min 21, P = 0.7). Liver DFA partitioning was unchanged, but liver fractional uptake of DFA tended to be increased. Very short-term caloric and saturated fat dietary restrictions do not lead to the same changes in organ-specific DFA metabolism as those associated with weight loss in subjects with IGT.

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PET Imaging of Myocardial Metabolism in Health and Disease

Gropler¹,

Peterson²

2019

Cardiac CT, PET &Amp; MR

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Organ-specific dietary fatty acid uptake in humans using positron emission tomography coupled to computed tomography

Cited by 78 publications

References 35 publications

Effect of Sex and Impaired Glucose Tolerance on Organ-Specific Dietary Fatty Acid Metabolism in Humans

Effect of Sex and Impaired Glucose Tolerance on Organ-Specific Dietary Fatty Acid Metabolism in Humans

Seven-Day Caloric and Saturated Fat Restriction Increases Myocardial Dietary Fatty Acid Partitioning in Impaired Glucose-Tolerant Subjects

PET Imaging of Myocardial Metabolism in Health and Disease

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