Organ-on-a-chip: A new tool for in vitro research

Yan, Jiasheng; Jie, Guo; Liu, Shan; Guo, Jinhong

doi:10.1016/j.bios.2022.114626

Cited by 30 publications

(18 citation statements)

References 168 publications

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“…The adoption of OOC technology by the committee of clinicians and the pharmaceutical industry urgently needs to overcome several challenges and obstacles [ 29 , 323 , 324 , 325 ]. Thus, in the past few years, the fever of start-ups in the field of innovative biomedical technologies has emerged and drowned the market.…”

Section: Organ-on-a-chip Platforms: a Glance On The Marketmentioning

confidence: 99%

Organs-on-Chips Platforms Are Everywhere: A Zoom on Biomedical Investigation

et al. 2022

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Over the decades, conventional in vitro culture systems and animal models have been used to study physiology, nutrient or drug metabolisms including mechanical and physiopathological aspects. However, there is an urgent need for Integrated Testing Strategies (ITS) and more sophisticated platforms and devices to approach the real complexity of human physiology and provide reliable extrapolations for clinical investigations and personalized medicine. Organ-on-a-chip (OOC), also known as a microphysiological system, is a state-of-the-art microfluidic cell culture technology that sums up cells or tissue-to-tissue interfaces, fluid flows, mechanical cues, and organ-level physiology, and it has been developed to fill the gap between in vitro experimental models and human pathophysiology. The wide range of OOC platforms involves the miniaturization of cell culture systems and enables a variety of novel experimental techniques. These range from modeling the independent effects of biophysical forces on cells to screening novel drugs in multi-organ microphysiological systems, all within microscale devices. As in living biosystems, the development of vascular structure is the salient feature common to almost all organ-on-a-chip platforms. Herein, we provide a snapshot of this fast-evolving sophisticated technology. We will review cutting-edge developments and advances in the OOC realm, discussing current applications in the biomedical field with a detailed description of how this technology has enabled the reconstruction of complex multi-scale and multifunctional matrices and platforms (at the cellular and tissular levels) leading to an acute understanding of the physiopathological features of human ailments and infections in vitro.

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Section: Organ-on-a-chip Platforms: a Glance On The Marketmentioning

confidence: 99%

Organs-on-Chips Platforms Are Everywhere: A Zoom on Biomedical Investigation

et al. 2022

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“…This technique has gradually become the most promising cell research model. 3D cultures, including spheroids, are nowadays recognized as a better model of the in vivo environment, whereas rare detailed cell death (including ferroptosis) assays are available for 3D cultures (Jung et al, 2022;Yan et al, 2022). Robin Demuynck's (Demuynck et al, 2020) employed 3D cells culture technology to demonstrate an assay that can effectively identify diverse types of cell death, including ferroptosis, and quantitatively assess cell death in tumor spheroids, the 3D Cell Death Assay (3DELTA).…”

Section: Quantitatively Determine Ferroptosis In Spheroidsmentioning

confidence: 99%

The three-dimension preclinical models for ferroptosis monitoring

Meng

Sun

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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As a new programmed cell death process, ferroptosis has shown great potential and uniqueness in experimental and treatment-resistant cancer models. Currently, the main tools for drug research targeting ferroptosis are tumor cells cultured in vitro and tumor models established in rodents. In contrast, increasing evidence indicates that reactivity may differ from modifications in mice or humans in the process of drug screening. With the blossoming of 3D culture technology, tumor organoid culture technology has gradually been utilized. Compared with traditional 2D culture and tumor tissue xenotransplantation, tumor organoids have a significantly higher success rate. They can be cultured quickly and at a lower cost, which is convenient for gene modification and large-scale drug screening. Thus, combining 3D cell culture technology, drug monitoring, and ferroptosis analysis is necessary to develop the impact of ferroptosis-related agents in tumor treatment.

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“…Organ-on-a-chip (OoC) platforms replicate tissue and miniaturized organs, while preserving tissue/organ-level function and homeostasis [ 7 ]. They are found on microfluidic devices with perfused chambers that range from micrometers to millimeters in size, and are fed by continuous media flow [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Microneedles in Advanced Microfluidic Systems: A Systematic Review throughout Lab and Organ-on-a-Chip Applications

et al. 2023

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Microneedles (MNs) have been widely used in biomedical applications for drug delivery and biomarker detection purposes. Furthermore, MNs can also be used as a stand-alone tool to be combined with microfluidic devices. For that purpose, lab- or organ-on-a-chip are being developed. This systematic review aims to summarize the most recent progress in these emerging systems, to identify their advantages and limitations, and discuss promising potential applications of MNs in microfluidics. Therefore, three databases were used to search papers of interest, and their selection was made following the guidelines for systematic reviews proposed by PRISMA. In the selected studies, the MNs type, fabrication strategy, materials, and function/application were evaluated. The literature reviewed showed that although the use of MNs for lab-on-a-chip has been more explored than for organ-on-a-chip, some recent studies have explored this applicability with great potential for the monitoring of organ models. Overall, it is shown that the presence of MNs in advanced microfluidic devices can simplify drug delivery and microinjection, as well as fluid extraction for biomarker detection by using integrated biosensors, which is a promising tool to precisely monitor, in real-time, different kinds of biomarkers in lab- and organ-on-a-chip platforms.

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Organ-on-a-chip: A new tool for in vitro research

Cited by 30 publications

References 168 publications

Organs-on-Chips Platforms Are Everywhere: A Zoom on Biomedical Investigation

Organs-on-Chips Platforms Are Everywhere: A Zoom on Biomedical Investigation

The three-dimension preclinical models for ferroptosis monitoring

Microneedles in Advanced Microfluidic Systems: A Systematic Review throughout Lab and Organ-on-a-Chip Applications

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