Orchestration of MUC2 — The key regulatory target of gut barrier and homeostasis: A review

Liu, Yaxin; Yu, Zihan; Zhu, Lanping; Ma, Shaoping; Luo, Yang; Liang, Huixi; Liu, Qinlingfei; Chen, Jihua; Guli, Sitan; Chen, Xin

doi:10.1016/j.ijbiomac.2023.123862

Cited by 22 publications

(12 citation statements)

References 211 publications

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“…Tight junctions establish a selectively permeable barrier, allowing nutrient absorption while preventing the intrusion of luminal pathogens. Moreover, a thick layer of mucus, produced by goblet cells, overlays the epithelial cells and serves as the first line of host defense . In this study, FMT from breast-fed piglets enhanced tight junctions, restored the MUC2 expression by augmenting the goblet cell population, and alleviated colonic injury in FF piglets.…”

Section: Discussionmentioning

confidence: 57%

“…Moreover, a thick layer of mucus, produced by goblet cells, overlays the epithelial cells and serves as the first line of host defense. 37 In this study, FMT from breast-fed piglets enhanced tight junctions, restored the MUC2 expression by augmenting the goblet cell population, and alleviated colonic injury in FF piglets. This may be attributed to 5-HIAA production mediated by colonic microbiota.…”

Section: Discussionmentioning

confidence: 96%

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Gut Microbiota-Derived 5-Hydroxyindoleacetic Acid Alleviates Diarrhea in Piglets via the Aryl Hydrocarbon Receptor Pathway

Han,

Liu,

Wang

et al. 2023

J. Agric. Food Chem.

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With the improvement in sow prolificacy, formula feeding has been increasingly used in the pig industry. Diarrhea remains a serious health concern in formula-fed (FF) piglets. Fecal microbiota transplantation (FMT) is an efficacious strategy to reshape gut microbiota and the metabolic profile for treating diarrhea. This study aims to investigate whether FMT from breast-fed piglets could alleviate diarrhea in FF piglets. The piglets were randomly assigned to the control (CON) group, FF group, and FMT group. Our results showed that FF piglets exhibited a higher diarrhea incidence, damaged colonic morphology, and disrupted barrier function. In contrast, FMT treatment normalized the morphology and barrier function. FMT suppressed the JNK/MAPK pathway and production of proinflammatory cytokines. Additionally, FF piglets had a lower abundance of the beneficial bacterial genus Bifidobacterium compared to CON piglets. Following FMT administration, Bifidobacterium was restored. Meanwhile, 5-HIAA, a metabolite of tryptophan, and AHR-responsive CYP1A1 and CYP1B1 were upregulated. Importantly, integrated multiomics analysis revealed a strong positive correlation between Bifidobacterium and 5-HIAA. In vitro, 5-HIAA supplementation reversed the LPS-induced disruption of tight junctions and production of proinflammatory cytokines in IPEC-J2 cells. In conclusion, FMT reduced diarrhea incidence and improved growth performance. The alleviative effect of FMT on diarrhea was associated with Bifidobacterium and 5-HIAA.

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Section: Discussionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 96%

Gut Microbiota-Derived 5-Hydroxyindoleacetic Acid Alleviates Diarrhea in Piglets via the Aryl Hydrocarbon Receptor Pathway

Han,

Liu,

Wang

et al. 2023

J. Agric. Food Chem.

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“…Here, the dietary fiber-mediated cecal microbiota samples of the broilers were cultured in a medium containing gallbladder bile acids, and the results showed that supplementation of arabinoxylan and resistant starch impacted bacterial metabolic pathways related to secondary bile acid biotransformation. It has been reported that bile acids act as signaling molecules to maintain intestinal mucus barrier homeostasis ( 50 , 51 ). Therefore, these findings collectively demonstrate that the protective effect of arabinoxylan and resistant starch in the intestinal mucus barrier was mediated by bile acid transformation.…”

Section: Discussionmentioning

confidence: 99%

Microbiota-accessible fiber activates short-chain fatty acid and bile acid metabolism to improve intestinal mucus barrier in broiler chickens

Yang,

Qin,

Sun

et al. 2024

Microbiol Spectr

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Dietary polysaccharides are closely associated with gut microbiota and intestinal function homeostasis. This study aimed to investigate the mechanisms by which the β-glucan, arabinoxylan, and resistant starch selectively modulate gut microbiota and microbiota-derived metabolites to protect the intestinal mucus barrier in broiler chicken. In the present study, cecal microbiota samples from 21-day-old broilers were cultured in vitro with β-glucan, arabinoxylan, or resistant starch as the sole carbon source. We found that β-glucan, arabinoxylan and resistant starch alter community structure and selectively target the enrichment of Bacteroides , Lactobacillus, Coprococcus , Butyricicoccus , Ruminococcus and Blautia , respectively. Notably, supplementing fiber-deficient diets with arabinoxylan and resistant starch, but not with β-glucan, improved the intestinal mucus barrier by cecal microbiota enrichment and increase in the concentration of microbiota-derived short-chain fatty acids (SCFAs). In addition, we illustrated through bacterial cultures in vitro that the supplementation of arabinoxylan and resistant starch resulted in a change in bacterial biotransformation of secondary bile acids. Our findings provide insight into how arabinoxylan and resistant starch may selectively target gut microbiota and mediate the production of SCFAs and bile acid biotransformation to improve intestinal mucus barrier function. IMPORTANCE The intestinal mucus barrier, located at the interface of the intestinal epithelium and the microbiota, is the first line of defense against pathogenic microorganisms and environmental antigens. Dietary polysaccharides, which act as microbiota-accessible fiber, play a key role in the regulation of intestinal microbial communities. However, the mechanism via which dietary fiber affects the intestinal mucus barrier through targeted regulation of the gut microbiota is not clear. This study provides fundamental evidence for the benefits of dietary fiber supplementation in broiler chickens through improvement in the intestinal mucus barrier by targeted regulation of the gut ecosystem. Our findings suggest that the microbiota-accessible fiber-gut microbiota-short-chain fatty acid/bile acid axis plays a key role in regulating intestinal function.

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“…MUC2 tends to show a positive correlation with the number and function of goblet cells, making it a specific marker indicative of goblet cell secretory activity. [ 58 ]. Our results showed that the intra-amniotic administration of a chia phenolic extract was not able to increase the number of goblet cells in the crypt ( Table 3 ).…”

Section: Discussionmentioning

confidence: 99%

Chia Phenolic Extract Appear to Improve Small Intestinal Functionality, Morphology, Bacterial Populations, and Inflammation Biomarkers In Vivo (Gallus gallus)

Mishima,

Martino,

Kolba

et al. 2023

Nutrients

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Phenolic compounds can act as a substrate for colonic resident microbiota. Once the metabolites are absorbed and distributed throughout the body, they can have diverse effects on the gut. The objective of this study was to evaluate the effects of the intra-amniotic administration of a chia phenolic extract on intestinal inflammation, intestinal barrier, brush border membrane functionality, intestinal microbiota, and morphology in vivo (Gallus gallus model). Cornish-cross fertile broiler eggs, at 17 days of embryonic incubation, were separated into groups as follows: non-injected (NI; this group did not receive an injection); 18 MΩ H2O (H2O; injected with ultrapure water), and 10 mg/mL (1%) chia phenolic extract (CPE; injected with phenolic extract diluted in ultrapure water). Immediately after hatch (21 days), chickens were euthanized and their small intestine, cecum, and cecum content were collected and analyzed. The chia phenolic extract reduced the tumor necrosis factor-alpha (TNF-α) and increased the sucrose isomaltase (SI) gene expression, reduced the Bifidobacterium and E. coli populations, reduced the Paneth cell diameter, increased depth crypt, and maintained villus height compared to the non-injected control group. Chia phenolic extract may be a promising beneficial compound for improving intestinal health, demonstrating positive changes in intestinal inflammation, functionality, microbiota, and morphology.

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Orchestration of MUC2 — The key regulatory target of gut barrier and homeostasis: A review

Cited by 22 publications

References 211 publications

Gut Microbiota-Derived 5-Hydroxyindoleacetic Acid Alleviates Diarrhea in Piglets via the Aryl Hydrocarbon Receptor Pathway

Gut Microbiota-Derived 5-Hydroxyindoleacetic Acid Alleviates Diarrhea in Piglets via the Aryl Hydrocarbon Receptor Pathway

Microbiota-accessible fiber activates short-chain fatty acid and bile acid metabolism to improve intestinal mucus barrier in broiler chickens

Chia Phenolic Extract Appear to Improve Small Intestinal Functionality, Morphology, Bacterial Populations, and Inflammation Biomarkers In Vivo (Gallus gallus)

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