Multifunctional graphene oxide (GO)/chitosan (CS) aerogel microspheres (GCAMs) with honeycomb-cobweb and radially oriented microchannel structures are prepared by combining electrospraying with freeze-casting to optimize adsorption performances of heavy metal ions and soluble organic pollutants. The GCAMs exhibit superior adsorption capacities of heavy metal ions of Pb(II), Cu(II), and Cr(VI), cationic dyes of methylene blue (MB) and Rhodamine B, anionic dyes of methyl orange and Eosin Y, and phenol. It takes only 5 min to reach 82 and 89% of equilibrium adsorption capacities for Cr(VI) (292.8 mg g) and MB (584.6 mg g), respectively, much shorter than the adsorption equilibrium time (75 h) of a GO/CS monolith. More importantly, the GCAMs maintain excellent adsorption capacity for six cycles of adsorption-desorption. The broad-spectrum, rapid, and reusable adsorption performance makes the GCAMs promising for highly efficient water treatments.

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Mouse caspase-11 and human caspase-4 and caspase-5 recognize cytosolic lipopolysaccharide (LPS) to induce pyroptosis by cleaving the pore-forming protein GSDMD [1][2][3][4][5] . This non-canonical inflammasome defends against Gram-negative bacteria 6,7 . Shigella flexneri, which causes bacillary dysentery, lives freely within the host cytosol where these caspases reside. However, the role of caspase-11mediated pyroptosis in S. flexneri infection is unknown. Here we show that caspase-11 did not protect mice from S. flexneri infection, in contrast to infection with another cytosolic bacterium, Burkholderia thailandensis 8 . S. flexneri evaded pyroptosis mediated by caspase-11 or caspase 4 (hereafter referred to as caspase-11/4) using a type III secretion system (T3SS) effector, OspC3. OspC3, but not its paralogues OspC1 and 2, covalently modified caspase-11/4; although it used the NAD + donor, this modification was not ADP-ribosylation. Biochemical dissections uncovered an ADPriboxanation modification on Arg314 and Arg310 in caspase-4 and caspase-11, respectively. The enzymatic activity was shared by OspC1 and 2, whose ankyrin-repeat domains, unlike that of OspC3, could not recognize caspase-11/4. ADP-riboxanation of the arginine blocked autoprocessing of caspase-4/11 as well as their recognition and cleavage of GSDMD. ADP-riboxanation of caspase-11 paralysed pyroptosis-mediated defence in Shigella-infected mice and mutation of ospC3 stimulated caspase-11and GSDMD-dependent anti-Shigella humoral immunity, generating a vaccine-like protective effect. Our study establishes ADP-riboxanation of arginine as a bacterial virulence mechanism that prevents LPS-induced pyroptosis.Intracellular S. flexneri infection causes shigellosis in humans. Although most intracellular bacteria reside in vacuoles, S. flexneri, like Burkholderia spp., live freely in the host cytosol, inevitably exposing their LPS to caspase-11/4. Wild-type (WT) mice, unlike Casp11 −/− mice, survived B. thailandensis infection 8 (Fig. 1a, Extended Data Fig. 1a). Mice are increasingly being used as a surrogate host for S. flexneri. Unexpectedly, both WT and Casp11 −/− mice succumbed to lethal S. flexneri infection (Fig. 1a) and tolerated similarly the low-dose challenge (Extended Data Fig. 1a). Given the absence of caspase-11-mediated protection, we assayed non-canonical inflammasome activation upon S. flexneri infection. Casp1 −/− immortalized bone marrow-derived macrophages (iBMDMs) were used to avoid interference by the canonical inflammasome. Although B. thailandensis and S. Typhimurium ΔsifA induced Casp11-dependent GSDMD cleavage and pyroptosis 8 , S. flexneri triggered little pyroptosis (Fig. 1b) despite a higher infection efficiency (Extended Data Fig. 1b). In epithelium-derived human SiHa and A431 cells, S. flexneri, unlike S. Typhimurium ΔsifA, also did not activate the caspase-4-GSDMD pyroptosis pathway (Fig. 1b, Extended Data Fig. 1c, d). Purified LPS from S. flexneri was highly pro-pyroptotic (Extended Data Fig. 1e). Thus, S. flexneri evaded cas...

As an adaptation to the daily light–dark (diel) cycle, cyanobacteria exhibit diurnal rhythms of gene expression and cell cycle. The light–dark cycle also affects the life cycle of viruses (cyanophages) that infect the unicellular picocyanobacteriaProchlorococcusandSynechococcus, which are the major primary producers in the oceans. For example, the adsorption of some cyanophages to the host cells depends on light, and the burst sizes of cyanophages are positively correlated to the length of light exposure during infection. Recent metatranscriptomic studies revealed transcriptional rhythms of field cyanophage populations. However, the underlying mechanism remains to be determined, as cyanophage laboratory cultures have not been shown to exhibit diurnal transcriptional rhythms. Here, we studied variation in infection patterns and gene expression ofProchlorococcusphages in laboratory culture conditions as a function of light. We found three distinct diel-dependent life history traits in dark conditions (diel traits): no adsorption (cyanophage P-HM2), adsorption but no replication (cyanophage P-SSM2), and replication (cyanophage P-SSP7). Under light–dark cycles, each cyanophage exhibited rhythmic transcript abundance, and cyanophages P-HM2 and P-SSM2 also exhibited rhythmic adsorption patterns. Finally, we show evidence to link the diurnal transcriptional rhythm of cyanophages to the photosynthetic activity of the host, thus providing a mechanistic explanation for the field observations of cyanophage transcriptional rhythms. Our study identifies that cultured viruses can exhibit diurnal rhythms during infection, which might impact cyanophage population-level dynamics in the oceans.

In this paper, we study a simple means for coordinating teams of simple agents. In particular, we study ant robots and how they can cover terrain once or repeatedly by leaving markings in the terrain, similar to what ants do. These markings can be sensed by all robots and allow them to cover terrain even if they do not communicate with each other except via the markings, do not have any kind of memory, do not know the terrain, cannot maintain maps of the terrain, nor plan complete paths. The robots do not even need to be localized, which completely eliminates solving difficult and time-consuming localization problems. In this paper, we use real-time heuristic search methods to implement ant robots and present a simulation study with several real-time heuristic search methods to study their properties for terrain coverage. Our experiments show that all of the realtime heuristic search methods robustly cover terrain even if the robots are moved without realizing this, some robots fail, and some markings get destroyed. These results demonstrate that terrain coverage with real-time heuristic search methods is an interesting alternative to more conventional terrain coverage methods.

ObjectiveAntimicrobial peptides (AMPs) play essential roles in maintaining gut health and are associated with IBD. This study is to elucidate the effect of angiogenin (ANG), an intestine-secreted AMP, on gut microbiota and its relevance with IBD.DesignThe effect of ANG on microbiota and its contribution to colitis were evaluated in different colitis models with co-housing and faecal microbiota transplantation. ANG-regulated bacteria were determined by 16S rDNA sequencing and their functions in colitis were analysed by bacterial colonisation. The species-specific antimicrobial activity of ANG and its underlying mechanism were further investigated with microbiological and biochemical methods. ANG level and the key bacteria were characterised in IBD faecal samples.ResultsANG regulated microbiota composition and inhibited intestinal inflammation. Specifically, Ang1 deficiency in mice led to a decrease in the protective gut commensal strains of Lachnospiraceae but an increase in the colitogenic strains of α-Proteobacteria. Direct binding of ANG to α-Proteobacteria resulted in lethal disruption of bacterial membrane integrity, and consequently promoted the growth of Lachnospiraceae, which otherwise was antagonised by α-Proteobacteria. Oral administration of ANG1 reversed the dysbiosis and attenuated the severity of colitis in Ang1-deficient mice. The correlation among ANG, the identified bacteria and IBD status was established in patients.ConclusionThese findings demonstrate a novel role of ANG in shaping gut microbe composition and thus maintaining gut health, suggesting that the ANG-microbiota axis could be developed as a potential preventive and/or therapeutic approach for dysbiosis-related gut diseases.