An isocratic high-performance liquid chromatographic (HPLC) method has been developed to determine benzoic acid, sorbic acid, and methyl and propyl esters of p-hydroxygenzoic acid (parabens) in orange juice. The HPLC system consists of a reversed-phase column (150 X 6.0 mm i.d.) packed with a rigid polystyrene-divinylbenzene gel, a solvent system of acetonitrile-0.05M KH2P04 (40:60) with flow rate of 1.0 mL/min and 230 nm detection. Including sample preparation, a complete determination can be accomplished in 30 min. The method recovered 93% or more of all preservatives with the exception of high concentrations (500 ppm) of parabens.