Determination of Food Preservatives in Orange Juice by Reversed‐Phase Liquid Chromatography

Abstract

An isocratic high-performance liquid chromatographic (HPLC) method has been developed to determine benzoic acid, sorbic acid, and methyl and propyl esters of p-hydroxygenzoic acid (parabens) in orange juice. The HPLC system consists of a reversed-phase column (150 X 6.0 mm i.d.) packed with a rigid polystyrene-divinylbenzene gel, a solvent system of acetonitrile-0.05M KH2P04 (40:60) with flow rate of 1.0 mL/min and 230 nm detection. Including sample preparation, a complete determination can be accomplished in 30 min. The method recovered 93% or more of all preservatives with the exception of high concentrations (500 ppm) of parabens.

A Comparison between UV Spectrophotometer and High-performance Liquid Chromatography Method for the Analysis of Sodium Benzoate and Potassium Sorbate in Food Products

“…Skin reactions such as rash, urticaria, and contact dermatitis have been reported after topical application of potassium sorbate and sodium benzoate-containing products (Soni et al 2005;Nair 2001). There are various methods for analysis of benzoates and sorbates in food products, such as thin layer chromatography, ultraviolet (UV) spectroscopy, high-performance liquid chromatography (HPLC), and GC (Bui and Cooper 1987;Burini and Damiani 1991;Castellari et al 1997;Chen and Fu 1995;Mannino and Cosio 1996;Lee et al 1986;Leuenberger et al 1979;Montaño et al 1995;Serrano et al 1991). The objective of the present study was to measure the concentration of sodium benzoate or potassium sorbate in food products such as cookie, yogurt diluted with water and cucumber pickle samples by using of HPLC and UV spectrophotometer to compare these two methods.…”

Simultaneous Determination of Four Preservatives in Foodstuffs by High Performance Liquid Chromatography

“…The most common analytical method for determination of BA, SA and parabens is reversed-phase HPLC (13)(14)(15)(16)(17)(18). Although other analytical methods such as TLC (19,20), capillary electrophoresis (21-24), gas chromatography, and spectrophotometry (25-28) have also been reported.…”