Orange Fruit Color in Capsicum due to Deletion of Capsanthin-capsorubin Synthesis Gene

Lang, Yaqin; Yanagawa, Satoshi; Sasanuma, Tsuneo; Sasakuma, Tetsuo

doi:10.1270/jsbbs.54.33

Cited by 48 publications

(41 citation statements)

References 15 publications

(13 reference statements)

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“…The Ccs gene is not expressed in orange fruits, which suggests that the orange colour may originate from two possible alleles that are either dominant or recessive for Ccs . Lang et al (2004) thought that the orange fruit colour is due to deletion of Ccs gene. A relationship between Ccs gene and a fruit colour shows that the normal expression of Ccs gene and a sufficient amount of substrates for Ccs are necessary for producing the red colour of pepper fruits, whereas lack of Ccs gene expression or deficiency in substrates lead to the orange or yellow colours (Lang et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Lang et al (2004) thought that the orange fruit colour is due to deletion of Ccs gene. A relationship between Ccs gene and a fruit colour shows that the normal expression of Ccs gene and a sufficient amount of substrates for Ccs are necessary for producing the red colour of pepper fruits, whereas lack of Ccs gene expression or deficiency in substrates lead to the orange or yellow colours (Lang et al 2004). Some studies suggested that the deletion of Ccs gene is not a prerequisite for the formation of yellow fruits, and indeed, Ccs gene was found in two yellow Capsicum cultivars (Ha et al 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Sequence analyses of Ccs gene further reveal two structural mutations in yellow pepper that may result in either a premature stop-codon or a frame-shift. Lang et al (2004) find out a deletion of an upstream region of Ccs gene in orange fruits. The study of Rodriguez-Uribe et al (2012) in cv.…”

Section: Introductionmentioning

confidence: 99%

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The relationship between red fruit colour formation and key genes of capsanthin biosynthesis pathway in Capsicum annuum

Tian¹,

Li²,

Shah³

et al. 2015

Biologia plant.

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Pepper (Capsicum annum L.) fruit colours vary from green, ivory, or yellow at the juvenile stage to red, orange, and yellow at the mature stage. Carotenoid accumulation causes fruit colour formation in ripe Capsicum fruits, and capsanthin is a main carotenoid in ripe red pepper fruits. In this study, pepper cultivars with red, yellow, and orange fruits were employed and four key genes named phytoene synthase (Psy), lycopene-β-cyclase (Lcyb), β-carotene hydroxylase (Crtz), and capsanthin/capsorubin synthase (Ccs) in the capsanthin biosynthetic pathway were analyzed. Results show that a clear expression of the Ccs gene in cv. CK7 (yellow) was detected, but the expressions of the Psy and Lcyb genes were low comparing with the other tested cultivars. In addition, the expression of the Ccs gene was not observed in cv. R37-1 (orange). Furthermore, though the four key genes (Psy, Lcyb, Crtz, and Ccs) were detected in red cultivar R15, the expressions of them were low. Sequence analysis further indicated that the Ccs genes in cv. CK7 and cv. R37-1 had a high sequence identity (97.36 %). Interestingly, amino acid sequences coded by the Ccs genes from cv. CK7, cv. R37-1, and cv. R15 were the same. The above results suggest that the genes Psy, Lcyb, Crtz, and Ccs were required for capsanthin synthesis in pepper, and their varied expression patterns result in the variety of pepper fruit colours.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The relationship between red fruit colour formation and key genes of capsanthin biosynthesis pathway in Capsicum annuum

Tian¹,

Li²,

Shah³

et al. 2015

Biologia plant.

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“…Total DNA and total RNA were extracted according to the method of Lang et al (2004), DNA was obtained from the leaf, and RNA from the leaf, flower, seed, pericarp and placenta in fruits harvested at various developmental stages.…”

Section: Genomic Pcr and Rt-pcr Amplificationmentioning

confidence: 99%

“…The extension of the 5′ cDNA end was carried out according to the manufacturer's protocol of '5′-Full RACE Core set' (TaKaRa). The nested PCR amplified products were cloned, and their sequences were determined as described in the report of Lang et al (2004). …”

Section: Rapid Amplification Of the 5′ Cdna Ends (5′ Race)mentioning

confidence: 99%

A Gene Encoding a Putative Acyl-transferase Involved in Pungency of Capsicum

et al. 2006

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Pungency in the fruit of pepper (Capsicum spp.) is due to the accumulation of capsaicinoids. We isolated two genes characterized as putative Capsicum acyl-transferase genes (Catf-1 and Catf-2) from the placenta of pungent pepper, which displayed consensus motifs of acyl-transferase, H***D and DFG*G. The expression pattern of Catf-1 coincided with the accumulation of capsaicinoids in pungent pepper. The Catf-1 was expressed in placenta of pungent pepper fruit, but not in other organs, such as leaf, flower, seed and pericarp. Accumulation of mRNA of Catf-1 varied with the developmental stages of the pungent pepper fruits. Genomic PCR and Southern blot studies revealed the presence of a sequence deletion at the 5′ end of the Catf-1 coding region in the non-pungent line. In the F 2 population obtained from a cross between a non-pungent strain ('mGTY-1') and a pungent strain ('277 long'), the genotype at the Catf-1 locus cosegregated with the phenotype for fruit pungency. These results suggest that Catf-1 is a candidate gene differentiating pungent from non-pungent peppers.

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