Orally Administrated Lactobacillus gasseri TMC0356 and Lactobacillus GG Alleviated Nasal Blockage of Guinea Pig with Allergic Rhinitis

Abstract

Lactobacillus GG (LGG) and L. gasseri TMC0356 (TMC0356) were investigated for their ability to alleviate nasal blockage associated with allergic rhinitis using a guinea pig model. The increases in sRaw at 10 min and 5 hr after the exposure of the nasal mucosa to OVA were significantly alleviated in the guinea pigs orally administrated with LGG and TMC0356 compared with those of the control (P<0.05 and P<0.01). The total numbers of leukocytes, particularly eosinophils and neutrophils from the nasal cavity lavage fluid, and the OVA‐specific IgE concentration in the serum were also decreased in the guinea pigs orally administrated with LGG and TMC0356, although the decreases were not statistically significant. These results suggest that LGG and TMC0356 can alleviate antigen‐induced nasal blockage in earlyphase and late‐phase inflammatory responses associated with allergic rhinitis.

Uso de probióticos na rinite alérgica

“…There was also a significant reduction in the specific IgE dosing for OVA (ovalbumin), at the end of 4 weeks, indicating that the administration of L-55 strains of Lactobacillus inhibited the IgE production in sensitized mice 23 . Kawase et al, 2007, used the TMC 0356 strain of Lactobacillus gasseri and GG Lactobacillus in guinea pigs sensitized by the Japanese Cedar pollen, in the dose of 10 3 CFU during 3 weeks, with the goal of assessing the presence of nasal obstruction induced by the intranasal administration of OVA and changes to the immune cells of the nasal lavage and observed that, in relation to air flow resistance, after nasal administration of Ovalbumin, there was an increase in the resistance in both guinea pigs; however, in those who received Lactobacillus, this resistance was lower when compared to the control group after 10 minutes of OVA administration, and these data were statistically significant. In relation to the cells, there was a reduction in the number of leucocytes, especially eosinophils and neutrophils, in the nasal lavage and serum reduction in the levels of specific OVA-IgE levels and, despite this data not being significant in the study, they suggest a considerable action of these Lactobacillus, thus requiring further studies for such confirmation 24 .…”

“…Kawase et al, 2007, used the TMC 0356 strain of Lactobacillus gasseri and GG Lactobacillus in guinea pigs sensitized by the Japanese Cedar pollen, in the dose of 10 3 CFU during 3 weeks, with the goal of assessing the presence of nasal obstruction induced by the intranasal administration of OVA and changes to the immune cells of the nasal lavage and observed that, in relation to air flow resistance, after nasal administration of Ovalbumin, there was an increase in the resistance in both guinea pigs; however, in those who received Lactobacillus, this resistance was lower when compared to the control group after 10 minutes of OVA administration, and these data were statistically significant. In relation to the cells, there was a reduction in the number of leucocytes, especially eosinophils and neutrophils, in the nasal lavage and serum reduction in the levels of specific OVA-IgE levels and, despite this data not being significant in the study, they suggest a considerable action of these Lactobacillus, thus requiring further studies for such confirmation 24 . Rasche et al, 2007, co-stimulated peripheral mononuclear cells of individuals allergic to grass pollen (n=10) and those non-allergic (n=19) with inactive Lactobacillus acidophilus and the non-pathogenic Nissle strain of Escherichia coli and observed that both bacteria modulated the immune response from changes to the CD23 and expression of co-stimulatory molecules.…”

Establishment and characterization of an experimental mouse model of allergic rhinitis

“…However, there is no standardized method that can be followed universally. For example, the time point and duration for performing IP or IN varied from each other, not to mention the dosage of allergen they utilized [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16]. Besides, most methods used to sensitize the airway for assessment of hyperresponsiveness to antigen presentation were intended to establish an animal model of asthma and seldom for a model of AR.…”

“…Among them, mice are the most common animals used [2][3][4][5][6][7], followed by guinea pig [8][9][10][11][12]. Operating antigen, which is also called ''allergen'', is important to sensitize mice and render them ''allergic''.…”

“…In addition to mice, guinea pigs [8][9][10][11][12] or brown Norway rats [15,22] have also been used. Mice provide an easy means of assessing allergic symptoms such as sneezing or nasal rubbing (represents the motion due to itching), while rats may be a better choice if nasal obstruction is the target response for measure.…”