Orally Administered Fumonisins Affect Porcine Red Cell Membrane Sodium Pump Activity and Lipid Profile without Apparent Oxidative Damage

Szabó, A.; Ali, Omeralfaroug; Lóki, K.; Balogh, Krisztián; Mézes, Miklós; Bartók, Tibor; Horváth, Lajos; Kovács, Melinda

doi:10.3390/toxins12050318

Cited by 6 publications

(5 citation statements)

References 50 publications

(62 reference statements)

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“…In another study (Szabo et al., 2020), weaned piglets received for 21 days a control diet or a diet contaminated with 15 or 30 mg FBs (FB 1 + FB 2 + FB 3 ) /kg feed. When compared to control, final body weight was higher in the 30 mg/kg group.…”

Section: Assessmentmentioning

confidence: 99%

Assessment of information as regards the toxicity of fumonisins for pigs, poultry and horses

Schrenk¹,

Bignami²,

Bodin³

et al. 2022

EFS2

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In 2018, the EFSA Panel on Contaminants in the Food Chain (CONTAM) adopted a Scientific Opinion on the risks for animal health related to the presence of fumonisins, their modified forms and hidden forms in feed. A no observed adverse effect level (NOAEL) of 1 mg/kg feed was established for pigs.In poultry a NOAEL of 20 mg/kg feed and in horses a reference point for adverse animal health effect of 8.8 mg/kg feed was established, referred to as NOAEL. The European Commission (EC) requested EFSA to review the information regarding the toxicity of fumonisins for pigs, poultry and horses and to revise, if necessary, the established NOAELs. The EFSA CONTAM Panel considered that the term reference point (RP) for adverse animal health effects better reflects the uncertainties in the available studies. New evidence which had become available since the previous opinion allowed to revise an RP for adverse animal health effects for poultry from 20 mg/kg to 1 mg/kg feed (based on a LOAEL of 2.5 mg/kg feed for reduced intestinal crypt depth) and for horses from 8.8 to 1.0 mg/kg feed (based on case studies on equine leukoencephalomalacia (ELEM)). For pigs, the previously established NOAEL was confirmed as no further studies suitable for deriving an RP for adverse animal health effects could be identified. Based on exposure estimates performed in the previous opinion, the risk of adverse health effects of feeds containing FB1-3 was considered a concern for poultry, when taking into account the RP of 1 mg/kg feed for intestinal effects. For horses and other solipeds, the risk is considered low, although a large uncertainty associated with exposure was identified. The same conclusions apply to the sum of FB1-3 and their hidden forms.

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Section: Assessmentmentioning

confidence: 99%

Assessment of information as regards the toxicity of fumonisins for pigs, poultry and horses

Schrenk¹,

Bignami²,

Bodin³

et al. 2022

EFS2

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“…Among toxicological assessment parameters, FA metabolism is receiving profound interest, since lipids are associated with numerous diseases and can provide hints about cells’ physiological status [ 23 , 24 ]. The majority of in vivo and in vitro studies investigated the effects of individual FB 1 /FB on the hepatic membrane lipids of rats [ 23 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 ] and, to a lesser extent, on the blood of rabbits and the blood and liver of piglets [ 33 , 34 ]. Only a single study investigated the interaction of fusariotoxin (DON, ZEN and FB 1 ; individual, and binary and tertiary mixtures) with bilayer lipids of the liver and kidneys of male Wistar rats [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

The Effects of Mixed Fusarium Mycotoxins at EU-Permitted Feed Levels on Weaned Piglets’ Tissue Lipids

Ali

Mézes

Balogh

et al. 2021

Toxins

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At exactly the individual permitted EU-tolerance dietary limits, fumonisins (FB: 5 mg/kg diet) and mixed fusariotoxins (DZ: 0.9 mg deoxynivalenol + 0.1 mg zearalenone/kg diet, and FDZ: 5 mg fumonisins + 0.9 mg deoxynivalenol + 0.1 mg zearalenone/kg diet) were administered to piglets (n = 6/group) for three weeks. Bodyweights of intoxicated piglets increased, while feed conversion ratios decreased. In FDZ, both the absolute and relative weight of the liver decreased. In the renal-cellular membrane, the most pronounced alterations were in FDZ treatment, followed by individual FB exposure. In both treatments, high proportions of C20:0 and C22:0 with low fatty acid (FA) unsaturation were found. In hepatocyte phospholipids, FDZ toxins exerted antagonistic interactions, and FB had the strongest increasing effect on FA monounsaturation. Among all investigated organs, the spleen lipids were the least responsive, in which FDZ expressed synergistic reactions on C20:0 (↑ FDZ vs. FB) and C22:0 (↓ FDZ vs. DZ). The antioxidant defense of the kidney was depleted (↓ glutathione concentration by FB-exposure). Blood plasma indicated renal injury (profound increase of urea and creatinine in FB vs. DZ and FDZ). FB strongly increased total-cholesterol and low density lipoprotein concentrations, whereas FDZ synergistically increased gamma-glutamyltransferase, alkaline-phosphatase, calcium and phosphorus levels. Summarized, individual and combined multiple fusariotoxins modified the membrane lipid profile and antioxidant defense of splanchnic organs, and serum biochemicals, without retarding growth in piglets.

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“…Under FBs exposure, doses above and below EU established limits for pigs [8], histopathological abnormalities in the heart, lung, liver, kidney, and spleen have been recorded, whilst pulmonary edema (PPE) and cardiac dysfunction are the most frequent clinical signs in pigs [9]. Furthermore, FBs have been shown to disrupt the porcine intestinal barriers [10] and the membranes of erythrocytes by altering their lipid composition and Na + /K + ATPase activity [11].…”

Section: Introductionmentioning

confidence: 99%

Fumonisin B Series Mycotoxins’ Dose Dependent Effects on the Porcine Hepatic and Pulmonary Phospholipidome

Ali

Mézes

Balogh

et al. 2022

Toxins

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Male weaned piglets n = 6/group were fed Fumonisin B1+2+3 (FBs) mycotoxins at 0, 15, or 30 mg/kg diet for 3 weeks to assess the fatty acid (FA) composition of membrane lipid classes, lipid peroxidation, and histomorphological changes in the liver and lung. Growth performance and lipid peroxidation were unaltered, but histomorphological lesion scores increased in the liver. Linear dose–response was detected in liver phosphatidylcholines for C16:1n7, C18:1n9, and total monounsaturation and in lungs for C22:6n3, total n-3 and n-3:n-6, in pulmonary phosphatidylserines C20:0 and C24:0. Alterations associated with the highest FBs dose were detected in sphingomyelins (liver: total saturation ↓, total monounsaturation ↑), phosphatidylcholines (liver: total n-6 ↓, n-6:n-3 ↑; in lungs: total monounsaturation ↑, total polyunsaturation ↑), phosphatidylethanolamines (liver: total n-3 ↓; in lungs: total monounsaturation ↑ and n-6:n-3 ↑), phosphatidylserines (liver: n-6:n-3 ↑; in lungs: total saturation ↓, total polyunsatuartion ↑, and total n-6 and its ratio to n-3 ↑), and phosphatidylinositol (n-6:n-3 ↑; lungs: C22:1n9 ↑, C22:6n3 ↓, total saturation ↓, total monounsaturaion ↑). In conclusion, FBs exposures neither impaired growth nor induced substantial lipid peroxidation, but hepatotoxicity was proven with histopathological alterations at the applied exposure period and doses. FA results imply an enzymatic disturbance in FA metabolism, agreeing with earlier findings in rats.

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Orally Administered Fumonisins Affect Porcine Red Cell Membrane Sodium Pump Activity and Lipid Profile without Apparent Oxidative Damage

Cited by 6 publications

References 50 publications

Assessment of information as regards the toxicity of fumonisins for pigs, poultry and horses

Assessment of information as regards the toxicity of fumonisins for pigs, poultry and horses

The Effects of Mixed Fusarium Mycotoxins at EU-Permitted Feed Levels on Weaned Piglets’ Tissue Lipids

Fumonisin B Series Mycotoxins’ Dose Dependent Effects on the Porcine Hepatic and Pulmonary Phospholipidome

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