Oral Vaccination with Engineered Probiotic Limosilactobacillus reuteri Has Protective Effects against Localized and Systemic Staphylococcus aureus Infection

Pan, Na; Liu, Yang; Zhang, Haochi; Ying, Xu; Bao, Xuemei; Sheng, Shouxin; Liang, Yanchen; Liu, Bohui; Lyu, Yueqing; Li, Haotian; Ma, Fangfei; Pan, Haiting; Wang, Xiao

doi:10.1128/spectrum.03673-22

Cited by 4 publications

(3 citation statements)

References 67 publications

(79 reference statements)

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“…In a novel approach, Pan et al utilized Lactobacillus reuteri WXD171-IsdB, engineered as a delivery vessel displaying S. aureus antigen IsdB, which can tolerate the gastrointestinal environment to function as an oral vaccine with less potential risk than attenuated bacterial vectors such as diphtheria toxin. They found that L. reuteri WXD171-IsdB had a strong protective effect against S. aureus pulmonary infection, skin and soft tissue infection, systemic infection, and elicited enhanced humoral and importantly, cell-mediated immunity in murine models [ 40 ]. This novel method may serve to improve the immune response in humans and requires future research as a vaccine delivery system.…”

Section: Discussionmentioning

confidence: 99%

Atypical Staphylococcal Septic Arthritis in a Native Hip: A Case Report and Review

et al. 2023

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Septic arthritis is a synovial fluid and joint tissue infection with significant morbidity and mortality risk if not diagnosed and treated promptly. The most common pathogen to cause septic arthritis is Staphylococcus aureus, a Gram-positive bacterium. Although diagnostic criteria are in place to guide the diagnosis of staphylococcal septic arthritis, there is a lack of adequate sensitivity and specificity. Some patients present with atypical findings which make it difficult to diagnose and treat in time. In this paper, we present the case of a patient with an atypical presentation of recalcitrant staphylococcal septic arthritis in a native hip complicated by uncontrolled diabetes mellitus and tobacco usage. We review current literature on diagnosing S. aureus septic arthritis, novel diagnostic technique performance to guide future research and assist clinical suspicion, and current S. aureus vaccine development for at-risk patients.

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Section: Discussionmentioning

confidence: 99%

Atypical Staphylococcal Septic Arthritis in a Native Hip: A Case Report and Review

et al. 2023

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“…One study found that in vitro co-colonization of human PBMCs with S. aureus and Lactobacillus strains dampened IFNγ secretion in γδ T cells, MAIT cells, and NK cells stimulated by S. aureus cell free supernatants [68]. However, recent other studies utilize Lactobacillus recombinant strains as vaccine models against S. aureus due to the commensal bacteria's ability to induce a robust immune response [69,70]. Thus, it is evident that during S. aureus infection, lactobacilli have differential effects in vitro versus in vivo and must be studied contextually.…”

Section: Cutaneous Infectionmentioning

confidence: 99%

From Host Defense to Metabolic Signatures: Unveiling the Role of γδ T Cells in Bacterial Infections

Nanda,

Alphonse

2024

Biomolecules

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The growth of antibiotic-resistant bacterial infections necessitates focusing on host-derived immunotherapies. γδ T cells are an unconventional T cell subset, making up a relatively small portion of healthy circulating lymphocytes but a substantially increased proportion in mucosal and epithelial tissues. γδ T cells are activated and expanded in response to bacterial infection, having the capability to produce proinflammatory cytokines to recruit neutrophils and clear infection. They also play a significant role in dampening immune response to control inflammation and protecting the host against secondary challenge, making them promising targets when developing immunotherapy. Importantly, γδ T cells have differential metabolic states influencing their cytokine profile and subsequent inflammatory capacity. Though these differential metabolic states have not been well studied or reviewed in the context of bacterial infection, they are critical in understanding the mechanistic underpinnings of the host’s innate immune response. Therefore, this review will focus on the context-specific host defense conferred by γδ T cells during infection with Staphylococcus aureus, Streptococcus pneumoniae, Listeria monocytogenes, and Mycobacterium tuberculosis.

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“…Examples involve a recombinant, protein A-and IsdB-N2-containing five-antigen S. aureus vaccine (rFSAV) as well as SA4Ag, a multicomponent vaccine composed of capsular polysaccharide conjugates and recombinant forms of ClfA and the staphylococcal manganese transporter C (MntC; Creech et al, 2017 ; Frenck et al, 2017 ; Zeng et al, 2020 ). However, various clinical trials ended in failure due to adverse effects or limited efficacy in diseased patients ( Clegg et al, 2021 ), thereby asking for improved vaccination strategies that may encompass probiotic-based immunization ( Pan et al, 2023 ), advanced antibody engineering ( Chen et al, 2020 , 2022 ), or usage of live-attenuated vaccine platforms ( Cabral et al, 2017 ; Moscoso et al, 2018 ). Alternatively, chemical interference with the transpeptidase activity of sortase A may also help to limit S. aureus colonization and severity of staphylococcal disease.…”

Section: Targeting Cell Surface Proteins and Sortase A To Improve ...mentioning

confidence: 99%

Functional diversity of staphylococcal surface proteins at the host-microbe interface

Schwermann

Winstel

2023

Front. Microbiol.

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Surface proteins of Gram-positive pathogens are key determinants of virulence that substantially shape host-microbe interactions. Specifically, these proteins mediate host invasion and pathogen transmission, drive the acquisition of heme-iron from hemoproteins, and subvert innate and adaptive immune cell responses to push bacterial survival and pathogenesis in a hostile environment. Herein, we briefly review and highlight the multi-facetted roles of cell wall-anchored proteins of multidrug-resistant Staphylococcus aureus, a common etiological agent of purulent skin and soft tissue infections as well as severe systemic diseases in humans. In particular, we focus on the functional diversity of staphylococcal surface proteins and discuss their impact on the variety of clinical manifestations of S. aureus infections. We also describe mechanistic and underlying principles of staphylococcal surface protein-mediated immune evasion and coupled strategies S. aureus utilizes to paralyze patrolling neutrophils, macrophages, and other immune cells. Ultimately, we provide a systematic overview of novel therapeutic concepts and anti-infective strategies that aim at neutralizing S. aureus surface proteins or sortases, the molecular catalysts of protein anchoring in Gram-positive bacteria.

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Oral Vaccination with Engineered Probiotic Limosilactobacillus reuteri Has Protective Effects against Localized and Systemic Staphylococcus aureus Infection

Cited by 4 publications

References 67 publications

Atypical Staphylococcal Septic Arthritis in a Native Hip: A Case Report and Review

Atypical Staphylococcal Septic Arthritis in a Native Hip: A Case Report and Review

From Host Defense to Metabolic Signatures: Unveiling the Role of γδ T Cells in Bacterial Infections

Functional diversity of staphylococcal surface proteins at the host-microbe interface

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