“…In this study, the fibrosis is more prominently seen in superficial muscle layer using Haematoxylin and Eosin(44%) Van gieson's(46%) and Masson's trichrome(46%). Observation of observer I is in par with observer II which is Haematoxylin and Eosin (42%), Van gieson's(50%), Masson's trichrome(48%), respectively( Table 1, 2,3,5,6,8). H and E staining which is the most commonly used stain in pathology, highlighted the fibrotic changes at the deeper muscle fiber level in very few samples when compared to special stains such as Van Giesonsand Masson's trichrome which revealed fibrotic changes even in the lamina propria level (Table 1, 2,3).…”