Oral shedding of human herpesviruses in renal transplant recipients

Sarmento, Dmitry José de Santana; Tozetto‐Mendoza, Tânia Regina; Sumita, Laura Masami; Pierroti, Ligia; Pallos, Débora; Caliento, Rubens; Palmieri, Michelle; Martins, Victor Adriano de Oliveira; Gallottini, Marina; Pannuti, Cláudio Sérgio; Braz‐Silva, Paulo Henrique

doi:10.1007/s00784-017-2166-3

Cited by 14 publications

(21 citation statements)

References 27 publications

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“…El Hayderi et al suggested that nerve damage due to dental treatment causes HSV-1 reactivation and concluded that oral HSV shedding may be related to invasiveness of dental procedure, but fur- 27 ther studies are needed on this issue (2,37). De Santana Sarmento et al and Caliento et al concluded that kidney transplant recipients (adults and children, respectively) excreted herpes viruses more often than controls, especially HSV-1 and Epstein-Barr virus, with salivary shedding of herpes viruses being more frequent in patients with recent kidney transplantation (38,39). As we have argued elsewhere, these studies confirmed higher HSV-1 shedding in immunocompromised patients in comparison with healthy controls.…”

Section: Discussionsupporting

confidence: 81%

Assessment of Oral Shedding of Herpes Simplex Virus Before and After Endodontic and Fixed Partial Prosthodontics Treatment in Patients with Recurrent Herpes Simplex

Koopaie

Esmaily

Kharazifard

2018

Ann Mil Health Sci Res

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Background: Herpes simplex virus type 1 (HSV-1) as a member of the herpes virus family recurs in 25% of infected patients 1 -4 times a year. Reactivation of the virus in the oral mucosa is triggered by stress, sunray, menstruation and trauma to the face. Objectives: The current study aimed to evaluate the effect of fixed prosthetic treatment and root canal therapy on symptomatic and asymptomatic shedding of HSV-1 virus into the saliva. Methods: Twenty two patients with a history of recurrent herpes simplex infection were selected, 11 of whom underwent endodontic treatment and 11 received fixed prosthesis treatment. Saliva samples were obtained immediately before and three days after spitting method of collection. The samples were stored at -70°C and then examined via the polymerase chain reaction (PCR) technique in order to detect probable shedding of the virus. Results: In general, 72.7% (P = 0.09) and 63.6% (P = 0.09) of the samples were positive for HSV-1 in the endodontic and fixed prosthesis groups, respectively. Conclusions: The results of this study indicated that dental treatment could be considered as a risk factor for asymptomatic recurrence of HSV-1 virus in the oral cavity. Therefore, to prevent HSV infection, the use of infection control measures and prophylactic antiviral therapy is critical in immunocompromised patients.

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Section: Discussionsupporting

confidence: 81%

Assessment of Oral Shedding of Herpes Simplex Virus Before and After Endodontic and Fixed Partial Prosthodontics Treatment in Patients with Recurrent Herpes Simplex

Koopaie

Esmaily

Kharazifard

2018

Ann Mil Health Sci Res

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“…According to the authors, all cancer patients presented some degree of immunosuppression, which may explain the higher rates of EBV excretion. It is widely reported in the literature that oncological patients present immunosuppression at different degrees, which may explain the higher rates of EBV excretion, as previously observed in other immunosuppressed groups for several reasons [19,40]. …”

Section: Discussionmentioning

confidence: 93%

“…From these samples, according to a previous work of ours [13,19], we extracted DNA by using a semi-automatic method (NucliSENS® easyMAG® system, BioMérieux, Durham, NC) before amplification with PCR by using two sets of primers (i.e. HSVP1/P2 and VZVP1/P2) which correspond to a DNA sequence alignment from eight human herpesvirus described by Johnson [20] (Table 2).…”

Section: Methodsmentioning

confidence: 99%

“…The first set, HSVP1/P2, amplifies the subtypes HSV-1, HSV-2, EBV, CMV and HHV-8 and the second set, VZVP1/P2, amplifies the subtypes VZV, HHV-6 and HHV-7. The positive samples were submitted to enzymatic digestion with BamHI and BstUI restriction enzymes (New England Biolabs) for specific determination of each one of the eight herpesviruses, also described in a previous work of ours [13,19]. …”

Section: Methodsmentioning

confidence: 99%

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Oral shedding of human herpesviruses in patients undergoing radiotherapy/chemotherapy for head and neck squamous cell carcinoma is not affected by xerostomia

Palmieri

Ornaghi

Martins

et al. 2018

Journal of Oral Microbiology

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Background: Xerostomia is a very relevant and frequent complication of radiotherapy, causing the irradiated oral mucosa to be affected by bacterial, fungal and viral infections. Objective: The objective of this study was to evaluate a possible relationship between oral shedding of human herpesviruses and xerostomia in patients with squamous cell carcinoma of head and neck submitted to radio/chemotherapy. Methods: In this study, oral rinse samples were collected weekly from 20 patients during radiotherapy. The samples were submitted to PCR and enzymatic digestion for detection of human herpesviruses. Xerostomia was evaluated according to the Seminars in Radiation Oncology criteria. Results: There was a higher frequency of grade 1 xerostomia (51.4%), observed first in the 1st week of radiotherapy. In the 4th week of radiotherapy, all patients presented some degree of xerostomia. Analysis of herpesviruses showed oral shedding of EBV, HHV-6 and HHV-7 in all weeks. Considering all the periods, the highest frequency was in patients with EBV excretion (55.0%), which was significantly higher than that of other viruses. Conclusion: We observed that oral shedding of herpesviruses was not affected by xerostomia as there was a progression in their excretion, even with the evolution of xerostomia. This suggested that there is a local replication in the oral cavity that is not completely dependent of salivary excretion.

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“…The detection of herpesviruses in the saliva of renal transplant recipients has already been published. However, the pattern of salivary shedding of different human herpesviruses (HHV) in renal transplant patients remains uncertain …”

Section: Introductionmentioning

confidence: 99%

Salivary shedding of herpesviruses in renal transplant recipients

Sarmento

Caliento

Souza

et al. 2018

J of Invest & Clin Dent

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Oral shedding of human herpesviruses in renal transplant recipients

Abstract: The present findings support other longitudinal studies evaluating the relationship between oral shedding of human herpesviruses and clinical presence of active infection and renal transplant failure.

Cited by 14 publications

References 27 publications

Assessment of Oral Shedding of Herpes Simplex Virus Before and After Endodontic and Fixed Partial Prosthodontics Treatment in Patients with Recurrent Herpes Simplex

Assessment of Oral Shedding of Herpes Simplex Virus Before and After Endodontic and Fixed Partial Prosthodontics Treatment in Patients with Recurrent Herpes Simplex

Oral shedding of human herpesviruses in patients undergoing radiotherapy/chemotherapy for head and neck squamous cell carcinoma is not affected by xerostomia

Salivary shedding of herpesviruses in renal transplant recipients

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