Oral rinse MMP‐8 point‐of‐care immuno test identifies patients with strong periodontal inflammatory burden

Abstract

Oral rinse MMP-8 together with TIMP-1 analysis may have potential in complementary periodontal diagnostics. dentoELISA can be applied in quantitative oral rinse chair side biomarker diagnostics.

Clinical Relevance of the Advanced Microbiologic and Biochemical Investigations in Periodontal Diagnosis: A Critical Analysis

“…However, Mäntylä et al [92] suggested that it cannot be regarded categorically that all smoking periodontitis patients have lower levels of MMP-8 in GCF and further in oral rinse sample as they detected the highest MMP-8 GCF levels in smoking subjects with poor response to conventional periodontal treatment (scaling and root planning, SRP). Simultaneous analysis of MMP-8 and TIMP-1 proved beneficial [90]. Also the effect of MMP-8 inhibiting SDD medication could be monitored by analyzing the salivary and oral rinse MMP-8 levels to find out when a possible break in medication would be possible or when the medication should be taken again [95,96].…”

“…Antibodies applied in the immunoassays for the detection of MMPs and their regulators affected the measurement outcomes [45,90,91]. Nevertheless, particularly MMP-8 immunoassays and activity assays targeting PMN-type MMP-8 isoenzyme species in oral fluids have been found to be useful to differentiate periodontitis/peri-implantitis and gingivitis sites/patients as well as healthy sites/subjects [44,45,90,92]. These researchers utilized selective antibodies for detection of active MMP-8 in oral fluids for developing adjunctive diagnostic point-of-care/chair-side tests identifying sites susceptible for periodontitis progression and periodontitis affected patients [41].…”

The Role of Immuno-Inflammatory Response in the Pathogenesis of Chronic Periodontitis and Development of Chair-Side Point of Care Diagnostics

“…MMP-8, -9 and -13 , neutrophil elastase , 2 -macroglobulin, oxygen radical producer myeloperoxidase (MPO), pro-inflammatory mediators, (C Reactive Protein (CRP), IL-1 , TNF-, macrophage inflammatory protein -1) and bone remodeling markers (alkaline phosphatase, ICTP, RANKL, osteoprotegrin and osteocalcin). Among these periodontitis-related oral fluid biomarkers, especially MMP-8, -9 and -13, as well as MPO are potential candidates for chair-side point-of-care oral fluid assays Leppilahti et al 2011;. Low GCF volume and low biomarker levels in all oral fluids are characteristic during periodontal health, but the biomarker contents increase along with the severity of periodontal inflammation ).…”

Neutrophils alter epithelial response to Porphyromonas gingivalis in a gingival crevice model.

“…Although MMPs are known to contribute to the destruction of periodontal tissues in smokers and non-smokers alike (Ramseier et al, 2009;Leppilahti et al, 2011), the balance between MMPs and endogenous mediators has consistently been shown to be altered in cigarette smokers (Liede et al, 1999;Gursoy et al, 2010;Heikkinen et al, 2010). Recently, Mouzakiti et al (2011) have shown that TIMP-1 messenger RNA levels in human periodontal tissues removed from smokers with untreated chronic periodontitis are higher than in non-smokers.…”

“…Both MMP-8 and MMP-9 have been reported to reflect the inflammatory burden in periodontal tissues and so they represent biomarkers of periodontal disease (Ramseier et al, 2009;Leppilahti et al, 2011). Gingival crevicular modeling has permitted a number of novel insights into perturbations of the protease-antiprotease balance.…”