Oral Probiotics Alleviate Intestinal Dysbacteriosis for People Receiving Bowel Preparation

Deng, Xiaorong; Tian, Huakai; Yang, Rong; Han, Yiwen; Wei, Kehong; Zheng, Chaoran; Li, Zhaoxia; Chen, Tingtao

doi:10.3389/fmed.2020.00073

Cited by 23 publications

(28 citation statements)

References 43 publications

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“…Previous studies have shown that the decrease in the abundance of Bacteroides is closely related to poor health. Moreover, butyrate produced by Bacteroides plays an important role in maintaining the intestinal health of the host, exerting immunity, and anti-tumor effects ( Deng et al, 2020 ). In addition to these two stains, we confirmed important relative abundance of several microbial flora before and after CACC558 treatment in dogs.…”

Section: Discussionmentioning

confidence: 99%

Characterization and Functional Test of Canine Probiotics

Jang¹,

Son

Kim

et al. 2021

Front. Microbiol.

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Probiotics can modulate the composition of gut microbiota and benefit the host animal health in multiple ways. Lactic acid bacteria (LAB), mainly Lactobacillus and Bifidobacterium species, are well-known microbes with probiotic potential. In the present study, 88 microbial strains were isolated from canine feces and annotated. Among these, the four strains CACC517, 537, 558, and 566 were tested for probiotic characteristics, and their beneficial effects on hosts were evaluated both in vitro and in vivo; these strains exhibited antibiosis, antibiotic activity, acid and bile tolerance, and relative cell adhesion to the HT-29 monolayer cell line. Byproducts of these strains increased the viability and decreased oxidative stress in mouse and dog cell lines (RAW264.7 and DH82, respectively). Subsequently, when the probiotics were applied to the clinical trial, changes in microbial composition and relative abundance of bacterial strains were clearly observed in the experimental animals. Experimental groups before and after the application were obviously separated from PCA analysis of clinical results. Conclusively, these results could provide comprehensive understanding of the effects of probiotic strains (CACC517, 537, 558, and 566) and their industrial applications.

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Section: Discussionmentioning

confidence: 99%

Characterization and Functional Test of Canine Probiotics

Jang¹,

Son

Kim

et al. 2021

Front. Microbiol.

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“…FBD patients suffered an increase in the Firmicutes to Bacteroidetes ratio [ 32 , 33 ], and an increase in the Firmicutes to Bacteroidetes ratio may pose a potential risk to patients' health [ 34 ]. In our study, comparing with the control group, the Firmicutes to Bacteroidetes ratio (a reducing trend of Firmicutes abundance and a significant increase of Bacteroidetes abundance at the level of phylum) in the probiotics group significantly reduced after 4 weeks of live combined Bacillus subtilis and Enterococcus faecium supplementation, with significantly reducing abundance of Ruminococcus , Fusicatenibacter , and Eubacterium hallii group (Firmicutes) at the level of genus, which might help to improve the FBD patients' health.…”

Section: Discussionmentioning

confidence: 99%

“…In our study, comparing with the control group, the Firmicutes to Bacteroidetes ratio (a reducing trend of Firmicutes abundance and a significant increase of Bacteroidetes abundance at the level of phylum) in the probiotics group significantly reduced after 4 weeks of live combined Bacillus subtilis and Enterococcus faecium supplementation, with significantly reducing abundance of Ruminococcus , Fusicatenibacter , and Eubacterium hallii group (Firmicutes) at the level of genus, which might help to improve the FBD patients' health. Bacteroidetes play a vital role in degrading complex polysaccharides of cellulose, pectin, and xylan, which can help people absorb more energy from the diet, and butyrate produced by Bacteroidetes plays an important role in maintaining the intestinal health of the host, exerting immunity, and antitumor effect [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Combined Live Probiotics Alleviating the Gastrointestinal Symptoms of Functional Bowel Disorders

Shi

Gao

Zhang

2020

Gastroenterology Research and Practice

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Objective. Changes of the gut microbiota are related to the pathogenesis of functional bowel disorders (FBDs), and probiotic supplementation may be an effective treatment option. Therefore, we aimed to investigate the effect of combined live probiotics on the gastrointestinal symptoms of FBDs via altering the gut microbiota. Methods. Patients with the gastrointestinal symptoms of FBDs attending the Outpatient Department, from July to November 2019, were recruited. After the bowel preparation with polyethylene glycol electrolyte powder and colonoscopy, patients with normal result of colonoscopy were randomly divided into the probiotics group and control group. Patients in the probiotics group were prescribed with combined live Bacillus subtilis and Enterococcus faecium enteric-coated capsules for 4 weeks. Small intestinal bacteria overgrowth (SIBO) was measured by lactulose hydrogen breath test, and the microbial DNA was extracted from the fecal samples and the bacteria were classified by 16S rDNA gene amplicon sequencing. Results. Twenty-five patients of each group were recruited, and there was no significant difference between the probiotics and control groups on baseline gastrointestinal symptom rating scale (GSRS), positive rate of SIBO, and relative abundances of the gut microbiota at the phylum level. After 4 weeks of treatment, the values of the probiotics and control groups were as follows: GSRS 1.4±1.4 and 3.6±1.6 and positive rate of SIBO 28.0% and 56.0%, respectively. The median relative abundances of the gut microbiota were 1.01% and 5.03% Actinobacteria and 43.80% and 35.17% Bacteroidetes at the phylum level; 0.76% and 3.29% Bifidobacterium, 0.13% and 0.89% Cillinsella, 0.03% and 0.01% Enterococcus, 0.18% and 0.36% Lachnospiraceae, 0.10% and 0.16% Ruminococcus torques group, 1.31% and 2.44% Blautia, and 0.83% and 2.02% Fusicatenibacter at the genus level (P<0.05), respectively. Conclusion. Combined live probiotic supplementation after the bowel preparation can alter the gut microbiota, decontaminate SIBO, and alleviate the gastrointestinal symptoms of FBDs. This trial is registered with ChiCTR1900026472.

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“…Anaerobes play a dominant role in the gastrointestinal tract, among common anaerobes, Bifidobacterium is a functional gut microbiota, which is widely used in the treatment of inflammatory gastrointestinal diseases such as ulcerative colitis ( 155 , 156 ). TME in a low oxygen state creates a good growth environment for anaerobic bacteria ( 157 ). A study indicated that the accumulation of Bifidobacterium in TME can significantly improve the anti-tumor effect of anti-CD47 immunotherapy, which depends on the STING signal and the type I IFN within DC ( 158 ).…”

Section: The Effect Of Gut Microbiota In Cancer Immunotherapymentioning

confidence: 99%

“…The key role of B. fragilis is to restore the anti-CTLA-4 treatment response associated with Th1 immune responses in tumor-draining lymph nodes. environment for anaerobic bacteria (157). A study indicated that the accumulation of Bifidobacterium in TME can significantly improve the anti-tumor effect of anti-CD47 immunotherapy, which depends on the STING signal and the type I IFN within DC (158).…”

Section: Anti-cd47 Therapymentioning

confidence: 99%

Exploring the Emerging Role of the Gut Microbiota and Tumor Microenvironment in Cancer Immunotherapy

Qiu

Lin

et al. 2021

Front. Immunol.

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The tumor microenvironment (TME) is a complex ecosystem, which includes many different types of cells, abnormal vascular systems, and immunosuppressive cytokines. TME serves an important function in tumor tolerance and escapes from immune surveillance leading to tumor progression. Indeed, there is increasing evidence that gut microbiome is associated with cancer in a variety of ways, as specific microbial signatures are known to promote cancer development and influence safety, tolerability, and efficacy of therapies. Studies over the past five years have shown that the composition of the intestinal microbiota has a significant impact on the efficacy of anticancer immunosurveillance, which contribute to the therapeutic activity of cancer immunotherapies based on targeting cytotoxic T lymphocyte protein 4 (CTLA-4) or programmed cell death protein 1 (PD-1)–programmed cell death 1 ligand 1 (PD-L1) axis. In this review, we mainly discuss the impact of TME on cancer and immunotherapy through immune-related mechanisms. We subsequently discuss the influence of gut microbiota and its metabolites on the host immune system and the formation of TME. In addition, this review also summarizes the latest research on the role of gut microbiota in cancer immunotherapy.

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Oral Probiotics Alleviate Intestinal Dysbacteriosis for People Receiving Bowel Preparation

Cited by 23 publications

References 43 publications

Characterization and Functional Test of Canine Probiotics

Characterization and Functional Test of Canine Probiotics

Effect of Combined Live Probiotics Alleviating the Gastrointestinal Symptoms of Functional Bowel Disorders

Exploring the Emerging Role of the Gut Microbiota and Tumor Microenvironment in Cancer Immunotherapy

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