Oral immunization with recombinant Lactobacillus casei expressing OmpAI confers protection against Aeromonas veronii challenge in common carp, Cyprinus carpio

Zhang, Dong-Xing; Kang, Yuan-Huan; Chen, Long; Siddiqui, Shahrood Ahmed; Wang, Chunfeng; Ai-dong, Qian

doi:10.1016/j.fsi.2017.10.043

Cited by 59 publications

(45 citation statements)

References 54 publications

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“…Recent reports of infection in diverse aquatic species have prompted investigation into the bacterial pathogenesis in humans, animals, and fish [24,25]. Previously, research has shown that isolates could infect fish in the laboratory [26]. We investigated the burden of A. veronii infection in commercially available fish, including Carassius auratus, Cyprinus carpio, Ctenopharyngodon idella, and Silurus asotus.…”

Section: Discussionmentioning

confidence: 99%

Aeromonas veronii Infection in Commercial Freshwater Fish: A Potential Threat to Public Health

Li¹,

Raza

Yang³

et al. 2020

Animals

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Aeromonas veronii is an important pathogen causing freshwater fish sepsis and ulcer syndrome. An increasing number of cases have demonstrated its significance as an aquatic zoonotic agent. The purpose of this study was to ensure the safety of freshwater products by evaluating the infection status of edible freshwater fish. In this experiment, we isolated A. veronii from several species of apparently healthy freshwater fish, including Carassius auratus, Cyprinus carpio, Ctenopharyngodon idella, and Silurus asotus. A. veronii was identified through bacterial staining, culture characteristics, and 16S rDNA gene sequence. In addition, polymerase chain reaction (PCR) was used to investigate the distribution of seven major virulence genes, including aerolysin (aer: 88.51%), cytotoxic enterotoxin (act: 71.26%), serine proteinase (ser: 54.02%), adhesin (Aha: 40.23%), phospholipase (lip: 45.98%), nuclease (exu: 51.72%), and quorum sensing-controlled virulence factor (LuxS: 59.77%). In total, 496 strains of Aeromonas were isolated, including 87 strains of A. veronii. The isolates of A. veronii were Gram-negative, rod-shaped bacteria, and the colonies are yellow on Rimler-Shotts (RS) medium and showed greater than 99% homology with A. veronii ATCC35624 according to analyses of the 16S rDNA sequence. Nearly 50% of the A. veronii isolates carried at least four or more virulence genes, 25% of the isolates carried at least five types of virulence genes, and 59.77% isolates carried the LuxS gene, and the isolates carrying more virulence genes were found to be more virulent. These results are of great significance for further improving the food safety assessment of freshwater aquatic products.

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Section: Discussionmentioning

confidence: 99%

Aeromonas veronii Infection in Commercial Freshwater Fish: A Potential Threat to Public Health

Li¹,

Raza

Yang³

et al. 2020

Animals

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“…2019). Similarly, Zhang et al (2018) generated two recombinant Lactobacillus casei (surface‐displayed or secretory) expressing the OmpAI of A. veronii . The recombinant L. casei strains were directly delivered and could survive throughout the intestinal tract, and treated common carp showed an effective immune response and obtained 66.7% survival rate (Zhang et al .…”

Section: Application In Aquaculturementioning

confidence: 99%

Surface display system for probiotics and its application in aquaculture

Yao

Yang

Gao

et al. 2020

Reviews in Aquaculture

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Aquaculture is a fast‐growing and rapidly expanding industry in the world. Probiotics are widely used in aquaculture and have provided benefits to aquatic animal health, and it is also a promising alternative to antibiotics for control of fish diseases. With the development of biotechnology, new expression systems and novel techniques for the surface display of heterologous proteins in surface of probiotics cells have been developed. This review provides an overview of the systems and strategies for displaying functional proteins on the surface of probiotics commonly used in aquaculture, which are Bacillus subtilis, Bacillus thuringiensis, lactic acid bacteria represented by Lactococcus lactis and yeast. Their applications in aquaculture especially for oral vaccine development afforded by this technology and prospects and challenges associated with this technology are also highlighted.

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“…Lactobacillus casei CC16 was isolated from the intestine of common carp, and grown in De Man, Rogosa and Sharpe (MRS) medium (Thermo Fisher Scientific, Oxoid, UK) at 30 • C without shaking. The Escherichia coli-Lactobacillus shuttle vector pPG-1 and pPG-2 have been described in our previous study [44]. The competent cells, Escherichia coli MC1061, were grown in Luria-Bertani (LB) medium for cloning of the plasmids at 37 • C with shaking.…”

Section: Bacterial Strains Plasmids and Growth Conditionsmentioning

confidence: 99%

“…Xylose was added to the culture medium to a final concentration of 10 g/L to induce antigen expression. After induction at 30 • C for 10 h, bacterial cells or supernate (a 10-fold concentration) were examined by SDS-PAGE and transferred to nitrocellulose membrane as in our previous study [44].…”

Section: Western Blotting Assaymentioning

confidence: 99%

Oral Administration of Lactobacillus Casei Expressing Flagellin A Protein Confers Effective Protection against Aeromonas Veronii in Common Carp, Cyprinus Carpio

Tian

Kang

Chu

et al. 2019

IJMS

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Aeromonas veronii is a pathogen capable of infecting humans, livestock and aquatic animals, resulting in serious economic losses. In this study, two recombinant Lactobacillus casei expressing flagellin A (FlaA) of A. veronii, Lc-pPG-1-FlaA (surface-displayed) and Lc-pPG-2-FlaA (secretory) were constructed. The immune responses in fish administered with recombinant L. casei were evaluated. The two recombinant L. casei were orally administered to common carp, which stimulated high serum IgM and induced higher ACP, AKP, SOD and LYZ activity. Using qRT-PCR, the expression of IL-10, IL-8, IL-1β, TNF-α and IFN-γ in the tissue of fish immunized with recombinant L. casei was significantly (p < 0.05) upregulated, which indicated that recombinant L. casei could activate the innate immune system to trigger the cell immune response and inflammatory response. Furthermore, recombinant L. casei was able to survive the intestinal environment and colonize in intestine mucosal. The study showed that after being challenged by A. veronii, fish administered with Lc-pPG-1-FlaA (70%) and Lc-pPG-2-FlaA (50%) had higher survival rates compared to Lc-pPG and PBS, indicating that recombinant L. casei might prevent A. veronii infection by activating the immune system to trigger immune responses. We demonstrated that flagellin as an antigen of vaccine, is acceptable for preventing A. veronii infection in fish. The recombinant L. casei expressing FlaA may be a novel mucosal vaccine for treating and controlling A. veronii.

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Oral immunization with recombinant Lactobacillus casei expressing OmpAI confers protection against Aeromonas veronii challenge in common carp, Cyprinus carpio

Cited by 59 publications

References 54 publications

Aeromonas veronii Infection in Commercial Freshwater Fish: A Potential Threat to Public Health

Aeromonas veronii Infection in Commercial Freshwater Fish: A Potential Threat to Public Health

Surface display system for probiotics and its application in aquaculture

Oral Administration of Lactobacillus Casei Expressing Flagellin A Protein Confers Effective Protection against Aeromonas Veronii in Common Carp, Cyprinus Carpio

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