Oral Immunization with Recombinant
            <i>Lactococcus lactis</i>
            Expressing the Hemagglutinin of the Avian Influenza Virus Induces Mucosal and Systemic Immune Responses

Wang, Zhisheng; Gao, Junkai; Yu, Qinghua; Yang, Qian

doi:10.2217/fmb.12.69

Cited by 12 publications

(12 citation statements)

References 34 publications

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“…Intestinal, tracheal and genitourinary tract fluids were collected by rinsing the respective organs three times with 0.5 ml of ice-cold PBS containing protease inhibitors. Samples were centrifuged at 12 000 g for 15 min at 4°C, and the supernatants were stored at − 20°C until analyzed [15]. 5′-GAGCTCGGTCTGCAAATTGAATTCGAGTCTAGCATTCTG-3′(Sac I, EcoR I) ORF2F…”

Section: Mucosal Immunizations and Sample Collectionmentioning

confidence: 99%

“…However, there was an inevitable problem that needs to be pointed out-that the detectable levels of anti-ORF2 specific IgA and IgG were both low and kept short-term compared to relevant researches [14,15]. The major reasons are attributed to (i) low expression rate of the recombinant L. lactis NZ3900 resulted in adequate ORF2-antigen stimulation; (ii) weak colonization of the recombinant L. lactis NZ3900 in mice gut mucosa leads to shrinkage of duration of antigenic stimulation, and (iii) components of the recombinant L. lactis NZ3900 seem not competent enough to act as immunologic adjuvants.…”

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confidence: 99%

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Oral immunization with recombinant hepatitis E virus antigen displayed on the Lactococcus lactis surface enhances ORF2-specific mucosal and systemic immune responses in mice

Gao

Liu

et al. 2015

International Immunopharmacology

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Section: Mucosal Immunizations and Sample Collectionmentioning

confidence: 99%

mentioning

confidence: 99%

Oral immunization with recombinant hepatitis E virus antigen displayed on the Lactococcus lactis surface enhances ORF2-specific mucosal and systemic immune responses in mice

Gao

Liu

et al. 2015

International Immunopharmacology

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“…Nasal, tracheal, and lung lavage fluid samples were obtained by washing the organs with 0.5 ml, 0.2 ml, and 0.5 ml sterile phosphate-buffered saline (PBS), respectively. Hemagglutination inhibition (HI) titers in serum were determined as previously described (27). Specific IgGs (total IgG, IgG1, and IgG2a/c) in serum and secretory IgA in lavage fluid samples were determined by enzyme-linked immunosorbent assay (ELISA) using horseradish peroxidase-conjugated anti-mouse total IgG, IgG1, IgG2a (Santa Cruz Biotechnology, Inc., CA), and IgA (Southern Biotech, Birmingham, AL, USA) as described previously (28).…”

Section: Animalsmentioning

confidence: 99%

CpG Oligodeoxynucleotides Facilitate Delivery of Whole Inactivated H9N2 Influenza Virus via Transepithelial Dendrites of Dendritic Cells in Nasal Mucosa

Qin

Yin

et al. 2015

J Virol

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The spread of the low-pathogenicity avian H9N2 influenza virus has seriously increased the risk of a new influenza pandemic. Although whole inactivated virus (WIV) vaccine via intranasal pathway is the effective method of blocking virus transmission, the mucosal barrier seems to be a major factor hampering its development. CpG oligodeoxynucleotides, a known adjuvant, can target downstream dendritic cells (DCs) and effectively enhance the mucosal and systemic immune responses. However, the ability of CpGs to assist H9N2 WIV in transepithelial transport remains unknown. Here, in vitro and in vivo, we showed that CpGs provided assistance for H9N2 WIV in recruiting DCs to the nasal epithelial cells (ECs) and forming transepithelial dendrites (TEDs) to capture luminal viruses. CD103؉ DCs participated in this process. Chemokine CCL20 from nasal ECs played a key role in driving DC recruitment and TED formation. Virus-loaded DCs quickly migrated into the draining cervical lymph nodes (CLNs) for antigen presentation. In addition, the competence of CpGs was independent of direct epithelial transport via the transcellular or paracellular pathway. Taken together, our data demonstrated that CpGs enhanced the transport of H9N2 WIV via TEDs of nasal DCs, which might be a novel mechanism for optimal adaptive immune responses. IMPORTANCE This paper demonstrates by both an in vivo and an in vitro coculture model that CpG oligodeoxynucleotides, known as an adjuvant generally targeting downstream immune responses, also are crucial for the transport of H9N2 WIV across nasal epithelial cells (ECs) via the uptake of transepithelial dendrites (TEDs).Our results prove for the first time to our knowledge that the immune-potentiating mechanism of CpGs is based on strengthening the transepithelial uptake of H9N2 WIV in nasal mucosa. These findings provide a fresh perspective for further improvement of intranasal influenza vaccines, which are urgently needed in the face of the potential threat of H9N2 influenza.T he prevention and control of influenza are becoming more and more urgent, especially given the recent avian influenza A (H7N9) outbreaks in China (1). This subtype is primarily reassorted with enzootic H9N2 viruses that have circulated widely among birds in the Far East and the Middle East since the late 1990s (2). Based on their safety profile, high immunogenicity, and the capability of establishing cross-protection at the pathogen's entry site and interrupting viral transmission (3-6), whole inactivated H9N2 influenza vaccines given via intranasal (i.n.) immunization are very effective for virus elimination. Nonetheless, the efficacy of intranasal immunization is currently poor, primarily because of the physiology of the nasal cavity. Antigens have to find their way to overcome a series of barriers (mucus, cilia, and compact epithelium) before they are absorbed into the body (7). Numerous studies have attempted to improve the effect of i.n. whole inactivated virus (WIV) influenza vaccines by using mucoadhesive particulate...

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“…For these reasons, L. lactis is widely used in fermented foods and dairy products. Studies of L. lactis in oral health have examined the inhibition of planktonic S. mutans growth and mucosal immune stimulation [22,23]. L. lactis HY 449 was isolated from contaminated milk products and contained the same fatty acid profiles as L. lactis ATCC 19435 as a type strain.…”

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confidence: 99%

Inhibitory Effect of Lactococcus lactis HY 449 on Cariogenic Biofilm

Kim

Lee

2016

Journal of Microbiology and Biotechnology

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Dental caries is caused by cariogenic biofilm, an oral biofilm including . Recently, the prevention of dental caries using various probiotics has been attempted. HY 449 is a probiotic bacterium. The aim of this study was to investigate the effect of HY 449 on cariogenic biofilm and to analyze its inhibitory mechanisms. Cariogenic biofilm was formed in the presence or absence of HY 449 and ATCC 19435, and analyzed with a confocal laser microscope. The formation of cariogenic biofilm was reduced in cultures spiked with both strains, and HY 449 exhibited more inhibitory effects than ATCC 19435. In order to analyze and to compare the inhibitory mechanisms, the antibacterial activity of the spent culture medium from both strains against was investigated, and the expression of glucosyltransferases () of was then analyzed by real-time RT-PCR. In addition, the sucrose fermentation ability of both strains was examined. Both strains showed antibacterial activity and inhibited the expression of, and the difference between both strains did not show. In the case of sucrose-fermenting ability, HY 449 fermented sucrose but ATCC 19435 did not. HY 449 inhibited the uptake of sucrose and the expression of , whereby the development of cariogenic biofilm may be inhibited. In conclusion, HY 449 may be a useful probiotic for the prevention of dental caries.

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Oral Immunization with Recombinant Lactococcus lactis Expressing the Hemagglutinin of the Avian Influenza Virus Induces Mucosal and Systemic Immune Responses

Abstract: LL36EH triggered the anti-HA(1)-specific humoral and cellular immune responses and protective immunity. Therefore, oral immunization with LL36EH could be a valuable strategy against highly pathogenic avian influenza for humans and animals.

Cited by 12 publications

References 34 publications

Oral immunization with recombinant hepatitis E virus antigen displayed on the Lactococcus lactis surface enhances ORF2-specific mucosal and systemic immune responses in mice

Oral immunization with recombinant hepatitis E virus antigen displayed on the Lactococcus lactis surface enhances ORF2-specific mucosal and systemic immune responses in mice

CpG Oligodeoxynucleotides Facilitate Delivery of Whole Inactivated H9N2 Influenza Virus via Transepithelial Dendrites of Dendritic Cells in Nasal Mucosa

Inhibitory Effect of Lactococcus lactis HY 449 on Cariogenic Biofilm

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