Oral immunization of carps with chitosan–alginate microcapsule containing probiotic expressing spring viremia of carp virus (SVCV) G protein provides effective protection against SVCV infection

Jia, Shijie; Zhou, Kun; Pan, Ronghui; Wei, Jing; Liu, Zhongmei; Xu, Yigang

doi:10.1016/j.fsi.2020.07.052

Cited by 27 publications

(22 citation statements)

References 10 publications

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“…According to preliminary results of serum antibody production and previous researches (Guo et al, 2020;Jia, Zhou, et al, 2020;Qiu et al, 2019), the highest antibody titre appeared in about three weeks (21 days). Serum samples of 21 days after immunization are used for testing total antioxidant capacity (T-AOC), acid phosphatase (ACP), alkaline phosphatase (AKP) activities and superoxide dismutase (SOD), which were measured by using the assay kits (Jiancheng Bioengineering Institute) according to the method provided by the manufacturer.…”

Section: Immunological Assaysmentioning

confidence: 80%

PEG‐modified subunit vaccine encoding dominant epitope to enhance immune response against spring viraemia of carp virus

Duan

Zhao

Jin

et al. 2021

Journal of Fish Diseases

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Viral diseases exert a tremendous effect on aquaculture economics. Spring viraemia of carp (SVC) caused by spring viraemia of carp virus (SVCV) is one of the most serious aquatic viral diseases (Ahne et al., 2002). SVCV can infect almost all fish of cyprinids, including Cyprinus carpio, Danio rerio and Ctenophartngodon iddellus, and the mortality rate of juvenile fish is more than 90% (Ashraf et al., 2016; Embregts et al., 2019). SVCV, a cytopathic virus belonging to the genus Sprivivirus of the family Rhabdoviridae, is a single-stranded RNA virus with a genome length of 11,019 nucleotides, five open reading frames and five proteins: nucleoprotein (N), phosphoprotein (P), polymerase (L), matrix protein (M) and glycoprotein (G)

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Section: Immunological Assaysmentioning

confidence: 80%

PEG‐modified subunit vaccine encoding dominant epitope to enhance immune response against spring viraemia of carp virus

Duan

Zhao

Jin

et al. 2021

Journal of Fish Diseases

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“…It was earlier reported that chitosan and its derivatives possess antimicrobial properties against fungi [27][28][29][30][31], bacteria [32][33][34], and viruses [35][36][37][38]. However, the mechanism of their antimicrobial activity has not yet been fully explained.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from Hermetia illucens Relative to Crab Chitosan

et al. 2022

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This study shows the research on the depolymerisation of insect and crab chitosans using novel enzymes. Enzyme preparations containing recombinant chitinase Chi 418 from Trichoderma harzianum, chitinase Chi 403, and chitosanase Chi 402 from Myceliophthora thermophila, all belonging to the family GH18 of glycosyl hydrolases, were used to depolymerise a biopolymer, resulting in a range of chitosans with average molecular weights (Mw) of 6–21 kDa. The depolymerised chitosans obtained from crustaceans and insects were studied, and their antibacterial and antifungal properties were evaluated. The results proved the significance of the chitosan’s origin, showing the potential of Hermetia illucens as a new source of low molecular weight chitosan with an improved biological activity.

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“…Also, an effective protection rate of 71% in vaccinated carps and 53% in vaccinated koi until days 65 post-challenge was obtained [64]. A chitosan-alginate microcapsule probiotic L. plantarum expressing G protein of SVCV was constructed [65]. Common carps were primarily vaccinated by oral administration and after 14 days a booster vaccine was given and after 28 days, a second booster vaccine was administered.…”

Section: Spring Viremia Of Carp (Svc)mentioning

confidence: 99%

“…Common carps were primarily vaccinated by oral administration and after 14 days a booster vaccine was given and after 28 days, a second booster vaccine was administered. A significantly higher level of antigen-specific IgM antibodies was elicited and the vaccine provided effective protection against SVCV infection 42 days after the primary vaccination [65].…”

Section: Spring Viremia Of Carp (Svc)mentioning

confidence: 99%

Protection of Teleost Fish against Infectious Diseases through Oral Administration of Vaccines: Update 2021

Bøgwald

Dalmo

2021

IJMS

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Immersion and intraperitoneal injection are the two most common methods used for the vaccination of fish. Because both methods require that fish are handled and thereby stressed, oral administration of vaccines as feed supplements is desirable. In addition, in terms of revaccination (boosting) of adult fish held in net pens, oral administration of vaccines is probably the only feasible method to obtain proper protection against diseases over long periods of time. Oral vaccination is considered a suitable method for mass immunization of large and stress-sensitive fish populations. Moreover, oral vaccines may preferably induce mucosal immunity, which is especially important to fish. Experimental oral vaccine formulations include both non-encapsulated and encapsulated antigens, viruses and bacteria. To develop an effective oral vaccine, the desired antigens must be protected against the harsh environments in the stomach and gut so they can remain intact when they reach the lower gut/intestine where they normally are absorbed and transported to immune cells. The most commonly used encapsulation method is the use of alginate microspheres that can effectively deliver vaccines to the intestine without degradation. Other encapsulation methods include chitosan encapsulation, poly D,L-lactide-co-glycolic acid and liposome encapsulation. Only a few commercial oral vaccines are available on the market, including those against infectious pancreatic necrosis virus (IPNV), Spring viremia carp virus (SVCV), infectious salmon anaemia virus (ISAV) and Piscirickettsia salmonis. This review highlights recent developments of oral vaccination in teleost fish.

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Oral immunization of carps with chitosan–alginate microcapsule containing probiotic expressing spring viremia of carp virus (SVCV) G protein provides effective protection against SVCV infection

Cited by 27 publications

References 10 publications

PEG‐modified subunit vaccine encoding dominant epitope to enhance immune response against spring viraemia of carp virus

PEG‐modified subunit vaccine encoding dominant epitope to enhance immune response against spring viraemia of carp virus

Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from Hermetia illucens Relative to Crab Chitosan

Protection of Teleost Fish against Infectious Diseases through Oral Administration of Vaccines: Update 2021

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