Oral HPV16 Prevalence in Oral Potentially Malignant Disorders and Oral Cavity Cancers

Tang, Kai Dun; Menezes, Lilian; Baeten, Kurt; Walsh, L. J.; Whitfield, Bernard; Batstone, Martin; Kenny, Liz; Frazer, Ian H.; Scheper, Gert C.; Punyadeera, Chamindie

doi:10.3390/biom10020223

Cited by 24 publications

(22 citation statements)

References 59 publications

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“…Salivary oral rinse samples of this individual were collected at baseline, 6, 12, 36 month, and 2 weeks after his bilateral tonsillectomy using previously published method (8)(9)(10). Briefly, participants were asked to swish and gargle for 1-2 min with 2 × 10 mL volumes of 0.9% saline, prior to expectorating the rinse sample into a 50 mL falcon tube.…”

Section: Clinical Specimens' Collection and Processingmentioning

confidence: 99%

An Occult HPV-Driven Oropharyngeal Squamous Cell Carcinoma Discovered Through a Saliva Test

et al. 2020

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Section: Clinical Specimens' Collection and Processingmentioning

confidence: 99%

An Occult HPV-Driven Oropharyngeal Squamous Cell Carcinoma Discovered Through a Saliva Test

et al. 2020

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“…A growing body of evidence has suggested various means for establishing reliable and effective biomarkers for liquid biopsy screenings [ 24 , 25 ]. Due to the variability in standardized biomarkers, such as oral human papillomavirus (HPV) infection [ 26 , 27 ], focus has recently been placed on validation of exosomal and extracellular vesicle-derived microRNAs as potential biomarkers for liquid biopsy cancer screening, including those of oral cancers [ 28 , 29 ]. Based on this information, the primary objective of this study was to determine if oral cancers produce exosomes or extracellular vesicles containing miR-365, which might be useful as a potential screening tool, as was recently demonstrated in advanced breast cancers [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

miR-365 (microRNA): Potential Biomarker in Oral Squamous Cell Carcinoma Exosomes and Extracellular Vesicles

Coon

Kingsley

Howard

2020

IJMS

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Introduction: miR-365 is a non-coding microRNA that regulates transcription and has been demonstrated to promote oncogenesis and metastasis in some cancers, while suppressing these effects in others. Many microRNAs are produced and then exported extracellularly in exosomes, which are small extracellular vesicles ranging from 30 to 100 nm that are found in eukaryotic fluids and facilitate many cellular functions. Exosomes and extracellular vesicles are produced by many cell types, including oral cancer cells—although no study to date has evaluated miR-365 and oral cancer exosomes or extracellular vesicles. Based on this information, our research question was to evaluate whether oral cancers produce exosomes or extracellular vesicles containing miR-365. Materials and Methods: Two commercially available oral cancer cell lines (SCC25 and CAL27) and a normal oral keratinocyte (OKF4) were grown in serum-free media, supplemented with exosome-depleted fetal bovine serum. Extracellular vesicles and exosomes were then isolated using the Invitrogen total exosome RNA and protein isolation kit for processing using the hsa-miR-365a-5p microRNA qPCR assay kit. Results: RNA was successfully isolated from the exosome-depleted supernatant from each cell line—SCC9, SCC15, SCC25, and CAL27 (oral squamous cell carcinomas) and OKF4 (oral epithelial cell line). Relative concentrations of RNA were similar among each cell line, which were not significantly different, p = 0.233. RNA quality was established by A260:A280 absorbance using a NanoDrop, revealing purity ranging 1.73–1.86. Expression of miR-16 was used to confirm the presence of microRNA from the extracted exosomes and extracellular vesicles. The presence of miR-365 was then confirmed and normalized to miR-16 expression, which demonstrated an increased level of miR-365 in both CAL27 and SCC25. In addition, the normalized relative quantity (RQ) for miR-365 exhibited greater variation among SCC25 (1.382–4.363) than CAL27 cells (1.248–1.536). Conclusions: These results confirm that miR-365 is not only expressed in oral cancer cell lines, but also is subsequently exported into exosomes and extracellular vesicles derived from these cultures. These data may help to contextualize the potential for this microRNA to contribute to the phenotypes and behaviors of oral cancers that express this microRNA. Future research will begin to investigate these potential mechanisms and pathways and to determine if miR-365 may be useful as an oral cancer biomarker for salivary or liquid biopsies.

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“…Furthermore, salivary HPV DNA has demonstrated to be a good marker for detecting HPV in oropharyngeal cancer, as a high agreement between salivary HPV16 DNA infection and tumor p16 expression has been observed [51][52][53]. However, a recent study revealed a lower sensitivity for identifying p16-positive oral cancer patients through salivary HPV, which may indicate a limited involvement of HPV16 in oral carcinogenesis [54]. Interestingly, our study reviewed the different salivary HPV genotypes identified in oral and oropharyngeal carcinomas, providing additional evidence on the co-existence of multiple HPV types during carcinogenesis.…”

Section: Discussionmentioning

confidence: 99%

Association of Salivary Human Papillomavirus Infection and Oral and Oropharyngeal Cancer: A Meta-Analysis

Rapado‐González

Martínez-Reglero

Salgado‐Barreira

et al. 2020

JCM

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Background. Human papillomavirus (HPV) infection has been recognized as an important risk factor in cancer. The purpose of this systematic review and meta-analysis was to determine the prevalence and effect size of association between salivary HPV DNA and the risk of developing oral and oropharyngeal cancer. Methods. A systematic literature search of PubMed, EMBASE, Web of Science, LILACS, Scopus and the Cochrane Library was performed, without language restrictions or specified start date. Pooled data were analyzed by calculating odds ratios (ORs) and 95% confidence intervals (CIs). Quality assessment was performed using the Newcastle–Ottawa Scale (NOS). Results. A total of 1672 studies were screened and 14 met inclusion criteria for the meta-analysis. The overall prevalence of salivary HPV DNA for oral and oropharyngeal carcinoma was 43.2%, and the prevalence of salivary HPV16 genotype was 27.5%. Pooled results showed a significant association between salivary HPV and oral and oropharyngeal cancer (OR = 4.94; 2.82−8.67), oral cancer (OR = 2.58; 1.67−3.99) and oropharyngeal cancer (OR = 17.71; 6.42−48.84). Significant associations were also found between salivary HPV16 and oral and oropharyngeal cancer (OR = 10.07; 3.65−27.82), oral cancer (OR = 2.95; 1.23−7.08) and oropharyngeal cancer (OR = 38.50; 22.43−66.07). Conclusions. Our meta-analysis demonstrated the association between salivary HPV infection and the incidence of oral and oropharyngeal cancer indicating its value as a predictive indicator.

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Oral HPV16 Prevalence in Oral Potentially Malignant Disorders and Oral Cavity Cancers

Cited by 24 publications

References 59 publications

An Occult HPV-Driven Oropharyngeal Squamous Cell Carcinoma Discovered Through a Saliva Test

An Occult HPV-Driven Oropharyngeal Squamous Cell Carcinoma Discovered Through a Saliva Test

miR-365 (microRNA): Potential Biomarker in Oral Squamous Cell Carcinoma Exosomes and Extracellular Vesicles

Association of Salivary Human Papillomavirus Infection and Oral and Oropharyngeal Cancer: A Meta-Analysis

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