Oral Grapeseed Oil and Sesame Oil in Experimental Acetic Acid-Induced Ulcerative Colitis in Rat

Abstract

BACKGROUNDInflammatory bowel diseases contain two digestive system diseases, ulcerative colitis (UC) and Crohn’s disease with unclear causes. The aim of present study was to investigate the therapeutic effects of administration of the Sesame oil (SO) and grape seed oil (GSO) as enema route in rats suffering from experimental acetic acid induced UC. METHODSEighty male rats were randomly allocated into 8 equal groups as health control (HC1) without any disease treated with 1 ml of normal saline as enema; HC2 received SO; HC3 received GSO; negative control (NC) with induced UC receiving 1 ml of normal saline as enema; and positive control (PC) with induced UC treated by asacol. All treatments were performed identically with 4 mg/kg of medication except for asacol that was 100 mg/kg for 7 days. The weight changes was recorded after seven days. The serum levels of malondialdehyde (MDA), total antioxidant capacity (TAC), interleukin-6, and c-reactive protein (CRP) were measured. Colon macroscopic and microscopic histological changes were also measured at the end of 7th day. RESULTSNo significant changes were detected in weight in neither groups on day 0 nor at the end of study. No beneficial effects were seen for all treatments regarding healing process and the decrease in inflammation. Between treatment groups, the lowest MDA (7.40±0.98 U/ml), CRP (83.20±10.01 mg/l) and IL-6 levels (130.86±10.70 mU/ml) and highest TAC (1.91±0.43 mmol/l) belonged to GSO group. CONCLUSIONGSO enema alone can be considered as a treatment of choice for UC due to its antioxidant properties.

Biochemical, histopathological and histochemical effects of Vitis vinifera L extract on acetic acid-induced colitis.

“…In the current study, intrarectal administration of 3% acetic acid caused induction of UC, and there was marked decrease in BW of animals in colitis group in agreement with the results of the previous studies [1,17]. The BW reduction of animals is indicative of their weakened state due to colitis.…”

“…The histochemical alterations observed due to acetic acid injection was compensated for after GSE treatment due to scavenging free radicals and restoration of normal tissue structure which reflected on the different biochemical activities regarding the synthesis of macromolecules such as proteins and polysaccharides. Similar observations were recorded in rats according to a very recent article [17,39].…”

“…In several studies, acetic acid was used as a model for induction of UC [17,18] where acetic acid causes colonic epithelial lesions, necrosis, and leukocyte infiltration to the damaged colon [19]. Moreover, the advantages of acetic acid-induced colitis are its low cost and the ease of administration.…”

The potential anti-inflammatory effect of Omega-3 polyunsaturated fatty acids on experimentally induced ulcerative colitis in rats

“…Inflammatory bowel diseases (IBD) is classified into two diseases are ulcerative colitis (UC) and Crohn's disease (CD) are multi-factorial, relapsing disorders of the gastrointestinal tract. Although, the etiology of these diseases involves altered immune responses, epithelial dysfunction, environmental factors, and nutrition (Banerjee et al,2019).Ulcerative colitis is characterized by intestinal mucosa inflammation with an extension from the rectum to the colon in a uniform manner (Hosseinzadeh et al,2017).the present study, the experimental induction of colitis with instillation of 3 ml of 3% acetic acid into the colon was successfully achieved and confirmed by macroscopic damage and histopathological findings such as congestion, haemorrhages, edema, and leukocytic infiltration. This result is in agreement with previous reports in animal models of colitis, which demonstrated similar macroscopic and microscopic pictures and tissue damage after the induction of colitis using acetic acid (El-Akabawy and El-Sherif , 2019).…”

Anti-Inflammatory, Antioxidant, and Healing-Promoting Effects of Aloe vera Extract in the Experimental Colitis in Rats

“…IL‐6 is another predominant cytokine found in inflamed areas from UC patients, and the other predominant cytokines were related to a Th1 profile. Different studies confirmed that induced UC in rats increases immunomodulator indexes confirming that oxidative stress could be correlated to disease [ 36 , 64 – 67 ]. Under normal conditions, ROS is naturally neutralized by the endogenous antioxidant system, including GSH, SOD, CAT, and GPx.…”