Oral gargle-tumor biopsy human papillomavirus (HPV) agreement and associated factors among oropharyngeal squamous cell carcinoma (OPSCC) cases

Martin‐Gomez, Laura; Fulp, William J.; Schell, Michael J.; Sirak, Bradley; Abrahamsen, Martha; Isaacs-Soriano, Kimberly; Lörincz, Attila T.; Wenig, Bruce M.; Chung, Christine H.; Caudell, Jimmy J.; Giuliano, Anna R.

doi:10.1016/j.oraloncology.2019.03.019

Cited by 18 publications

(28 citation statements)

References 34 publications

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“…13,[15][16][17][18][19][20] In addition, we noted the performance of the oral HPV 16 test was significantly lower among younger cases and those diagnosed with earlier disease that can be treated with monotherapy. 20 Hence, additional biomarkers are needed to improve oral HPV 16 test characteristics to identify OPC early.…”

Section: Introductionmentioning

confidence: 76%

“…However, agreement between oral rinses and tumor HPV 16 DNA has been variable, ranging between 59 and 96%, with most studies reporting values of approximately 70–80% . In addition, we noted the performance of the oral HPV 16 test was significantly lower among younger cases and those diagnosed with earlier disease that can be treated with monotherapy . Hence, additional biomarkers are needed to improve oral HPV 16 test characteristics to identify OPC early.…”

Section: Introductionmentioning

confidence: 81%

“…Oral HPV 16 alone does not accurately reflect case status, and its performance is worse among younger cases and cases with early disease, the group of patients we seek to identify with screening . In the screening setting, some who test oral HPV 16 positive will not have cancer, and some testing oral HPV 16 negative will have cancer.…”

Section: Methodsmentioning

confidence: 99%

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Methylation of HPV 16 and EPB41L3 in oral gargles: Associations with oropharyngeal cancer detection and tumor characteristics

Giuliano

Nedjai

Lörincz

et al. 2019

Intl Journal of Cancer

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Oropharyngeal cancer (OPC) incidence is increasing significantly among men and often requires intensive therapy causing significant morbidity. Early detection of OPC is needed, when monotherapy can be safely delivered with less treatment‐associated morbidity, while maintaining high cure rates. We conducted a study of 101 pretreatment male OPC cases matched 1:1 to 101 disease‐free controls for age and smoking history. Oral gargles were collected from cases and controls with additional biopsies or aspirates from cases. The HPV SPF10‐LiPA25 PCR assay was utilized for HPV genotyping. Methylation of three CpG sites (438, 427 and 425) in the EPB41L3 gene and methylation status of the L1 (6,367, 6,389), L2 (4,257, 4,262, 4,266, 4,269, 4,275, 4,282) and E2 (3,412, 3,415, 3,417, 3,433, 3,436) CpG sites of HPV 16 positive specimens was assessed by pyrosequencing. Significant correlations were observed between tumor and oral specimens for all methylation biomarkers (p < 0.01). EPB41L3 and HPV 16 L1, L2 and E2 methylation were significantly (p < 0.0001) higher among cases than controls, regardless of early vs. late disease. When HPV 16 genes and EPB41L3 methylation status were combined in a logistic regression analysis, a sensitivity of 70.3% and a specificity of 90.9% were observed for the detection of OPC from an oral gargle. Our data suggest that methylation biomarkers measured in oral gargles may have utility in identifying OPC early. Future studies are needed to replicate these findings and to inform additional biomarkers that can maximize specificity and sensitivity for early OPC detection.

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Section: Introductionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 81%

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Methylation of HPV 16 and EPB41L3 in oral gargles: Associations with oropharyngeal cancer detection and tumor characteristics

Giuliano

Nedjai

Lörincz

et al. 2019

Intl Journal of Cancer

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“…Several authors have shown a significant correlation between HPV DNA detected in tissue and positivity for HPV DNA in saliva, suggesting the potential value of this biofluid for detecting HPV and thus predicting HPV-related head and neck carcinomas [1,50]. Furthermore, salivary HPV DNA has demonstrated to be a good marker for detecting HPV in oropharyngeal cancer, as a high agreement between salivary HPV16 DNA infection and tumor p16 expression has been observed [51][52][53]. However, a recent study revealed a lower sensitivity for identifying p16-positive oral cancer patients through salivary HPV, which may indicate a limited involvement of HPV16 in oral carcinogenesis [54].…”

Section: Discussionmentioning

confidence: 96%

“…Cont. OC, oral cancer; OPC, oropharynx cancer; PCR, polymerase chain reaction; qPCR, quantitative PCR; FAP-PCR, fluorescent arbitrarily primed PCR; NGS, next-generation sequencing; * HR-HPV other:31,33,35,39,45,51,52, 56, 58, 59, 66, and 68; ** HPV α-9 other than HPV16: 31,33,35,52,58, and 67; *** HPV other:6, 11, 18, 26, 34, 39, 40, 42, 44, 45, 51, 53, 54, 56, 59, 61, 62, 66, 68, 69, 70, 71, 72, 73, 81, 82, 83, 84, and 89.…”

mentioning

confidence: 99%

Association of Salivary Human Papillomavirus Infection and Oral and Oropharyngeal Cancer: A Meta-Analysis

Rapado‐González

Martínez-Reglero

Salgado‐Barreira

et al. 2020

JCM

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Background. Human papillomavirus (HPV) infection has been recognized as an important risk factor in cancer. The purpose of this systematic review and meta-analysis was to determine the prevalence and effect size of association between salivary HPV DNA and the risk of developing oral and oropharyngeal cancer. Methods. A systematic literature search of PubMed, EMBASE, Web of Science, LILACS, Scopus and the Cochrane Library was performed, without language restrictions or specified start date. Pooled data were analyzed by calculating odds ratios (ORs) and 95% confidence intervals (CIs). Quality assessment was performed using the Newcastle–Ottawa Scale (NOS). Results. A total of 1672 studies were screened and 14 met inclusion criteria for the meta-analysis. The overall prevalence of salivary HPV DNA for oral and oropharyngeal carcinoma was 43.2%, and the prevalence of salivary HPV16 genotype was 27.5%. Pooled results showed a significant association between salivary HPV and oral and oropharyngeal cancer (OR = 4.94; 2.82−8.67), oral cancer (OR = 2.58; 1.67−3.99) and oropharyngeal cancer (OR = 17.71; 6.42−48.84). Significant associations were also found between salivary HPV16 and oral and oropharyngeal cancer (OR = 10.07; 3.65−27.82), oral cancer (OR = 2.95; 1.23−7.08) and oropharyngeal cancer (OR = 38.50; 22.43−66.07). Conclusions. Our meta-analysis demonstrated the association between salivary HPV infection and the incidence of oral and oropharyngeal cancer indicating its value as a predictive indicator.

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Current salivary biomarkers for detection of human papilloma virus‐induced oropharyngeal squamous cell carcinoma

et al. 2021

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Human papilloma virus (HPV) infection is a key risk factor and etiology for oropharyngeal squamous cell carcinoma (OPSCC). HPV‐induced OPSCC is rapidly increasing in incidence, with men experiencing increased mortality. When identified at an early stage, HPV‐induced OPSCC can be successfully treated. Diagnosis of HPV‐related OPSCC relies on an expert physical examination and invasive biopsy. Since saliva bathes the oropharyngeal mucosa and can be collected noninvasively, saliva obtained via salivary risings is an attractive body fluid for early detection of HPV‐induced OPSCC. A plethora of DNA, RNA, and protein salivary biomarkers have been explored. This review discusses these markers and their robustness for detecting oncogenic HPV in OPSCC saliva samples. Methods detecting HPV DNA were more reliable than those detecting RNA, albeit both require time‐consuming analyses. Salivary HPV proteomics are a new, promising focus of HPV detection research, and while more practical, lag behind nucleic acid detection methods in their development.

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Oral gargle-tumor biopsy human papillomavirus (HPV) agreement and associated factors among oropharyngeal squamous cell carcinoma (OPSCC) cases

Cited by 18 publications

References 34 publications

Methylation of HPV 16 and EPB41L3 in oral gargles: Associations with oropharyngeal cancer detection and tumor characteristics

Methylation of HPV 16 and EPB41L3 in oral gargles: Associations with oropharyngeal cancer detection and tumor characteristics

Association of Salivary Human Papillomavirus Infection and Oral and Oropharyngeal Cancer: A Meta-Analysis

Current salivary biomarkers for detection of human papilloma virus‐induced oropharyngeal squamous cell carcinoma

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