Oral exposure to high-dose ethanol for 28 days in rats reduces neural stem cells and immediate nascent neural progenitor cells as well as FOS-expressing newborn granule cells in adult hippocampal neurogenesis

Takahashi, Yasunori; Okano, Hiromu; Takashima, Kazumi; Ojiro, Ryota; Tang, Qian; Ozawa, Shunsuke; Ogawa, Bunichiro; Woo, Gye‐Hyeong; Yoshida, Toshinori; Shibutani, Makoto

doi:10.1016/j.toxlet.2022.02.006

Cited by 7 publications

(6 citation statements)

References 57 publications

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“…Interestingly, multiple genes that were significant for a strain × treatment interaction and showed a fold-change greater than 1.5 are involved in apoptosis-related pathways and have previously been linked to FASD including Bcl2l11 [ 62 ], Dusp1 [ 62 ], Egr3 [ 62 , 63 ], Fgf1 [ 64 , 65 ] Jun [ 62 , 66 ], Pik3r1 [ 62 ] Tgfb3 [ 62 , 67 ], and Txnip [ 62 , 66 ]. Moreover, many genes that showed differential ethanol-induced gene expression changes have been previously linked to gene expression changes in the brain of B6 mice using the same ethanol exposure paradigm as used in the current study [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Genetics and Sex on Acute Gene Expression Changes in the Hippocampus Following Neonatal Ethanol Exposure in BXD Recombinant Inbred Mouse Strains

et al. 2022

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Fetal alcohol spectrum disorders (FASD) are prevalent neurodevelopmental disorders. Genetics have been shown to have a role in the severity of alcohol’s teratogenic effects on the developing brain. We previously identified recombinant inbred BXD mouse strains that show high (HCD) or low cell death (LCD) in the hippocampus following ethanol exposure. The present study aimed to identify gene networks that influence this susceptibility. On postnatal day 7 (3rd-trimester-equivalent), male and female neonates were treated with ethanol (5.0 g/kg) or saline, and hippocampi were collected 7hrs later. Using the Affymetrix microarray platform, ethanol-induced gene expression changes were identified in all strains with divergent expression sets found between sexes. Genes, such as Bcl2l11, Jun, and Tgfb3, showed significant strain-by-treatment interactions and were involved in many apoptosis pathways. Comparison of HCD versus LCD showed twice as many ethanol-induced genes changes in the HCD. Interestingly, these changes were regulated in the same direction suggesting (1) more perturbed effects in HCD compared to LCD and (2) limited gene expression changes that confer resistance to ethanol-induced cell death in LCD. These results demonstrate that genetic background and sex are important factors that affect differential cell death pathways after alcohol exposure during development that could have long-term consequences.

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Section: Discussionmentioning

confidence: 99%

Effects of Genetics and Sex on Acute Gene Expression Changes in the Hippocampus Following Neonatal Ethanol Exposure in BXD Recombinant Inbred Mouse Strains

et al. 2022

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“…32 Transforming growth factor B3 can be secreted by M2 microglia/peripheral macrophages. 33 In addition, platelets are the primary carrier of TGFβ in the body, and TGFB3 genes showed a positive correlation with the number of platelets in the blood. 34 Myosin light chain kinase is a calcium ion (Ca2+)-dependent enzyme, contributing to various physiological processes related to myosin activation, such as cell adhesion, migration, and division.…”

Section: Discussionmentioning

confidence: 99%

“…Transforming growth factor B3 has been reported to promote cell migration and invasion as a tumor promotor in many cancers like breast cancer, 30 lung cancer, 30 and colorectal cancer, 31 mainly by activating TGFB pathways and epithelial–mesenchymal transition 32 . Transforming growth factor B3 can be secreted by M2 microglia/peripheral macrophages 33 . In addition, platelets are the primary carrier of TGF‐β in the body, and TGFB3 genes showed a positive correlation with the number of platelets in the blood 34 …”

Section: Discussionmentioning

confidence: 99%

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Development and experimental validation of an M2 macrophage and platelet‐associated gene signature to predict prognosis and immunotherapy sensitivity in bladder cancer

Muhuitijiang,

Zhou,

Zhou

et al. 2024

Cancer Science

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Platelets and M2 macrophages both play crucial roles in tumorigenesis, but their relationship and the prognosis value of the relative genes in bladder cancer (BLCA) remain obscure. In the present study, we found that platelets stimulated by BLCA cell lines could promote M2 macrophage polarization, and platelets were significantly associated with the infiltration of M2 macrophages in BLCA samples. Through the bioinformatic analyses, A2M, TGFB3, and MYLK, which were associated with platelets and M2 macrophages, were identified and verified in vitro and then included in the predictive model. A platelet and M2 macrophage‐related gene signature was constructed to evaluate the prognosis and immunotherapeutic sensitivity, helping to guide personalized treatment and to disclose the underlying mechanisms.

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“…In vitro and in vivo studies have shown that prenatal alcohol exposure impairs the development of the newly formed cortex in the developing fetus [110,111], and chronic alcohol exposure reduces neurogenesis and enhances cell death in adolescents; these changes may not be alleviated until a substantial period of time after alcohol cessation [112,113]. High-dose alcohol exposure in young adult rats suppresses hippocampal neurogenesis, including by decreasing the number of type-1 NSCs and type-2a NPCs in the DG subgranular zone and FOS+ granule cells, and reduces synaptic plasticity [114]. However, the mechanisms underlying neurogenesis impairment following alcohol consumption exposure remain unclear.…”

Section: Alcohol and Stressmentioning

confidence: 99%

The Effects of Extrinsic and Intrinsic Factors on Neurogenesis

Jiang

Jang

Zeng

2023

Cells

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In the mammalian brain, neurogenesis is maintained throughout adulthood primarily in two typical niches, the subgranular zone (SGZ) of the dentate gyrus and the subventricular zone (SVZ) of the lateral ventricles and in other nonclassic neurogenic areas (e.g., the amygdala and striatum). During prenatal and early postnatal development, neural stem cells (NSCs) differentiate into neurons and migrate to appropriate areas such as the olfactory bulb where they integrate into existing neural networks; these phenomena constitute the multistep process of neurogenesis. Alterations in any of these processes impair neurogenesis and may even lead to brain dysfunction, including cognitive impairment and neurodegeneration. Here, we first summarize the main properties of mammalian neurogenic niches to describe the cellular and molecular mechanisms of neurogenesis. Accumulating evidence indicates that neurogenesis plays an integral role in neuronal plasticity in the brain and cognition in the postnatal period. Given that neurogenesis can be highly modulated by a number of extrinsic and intrinsic factors, we discuss the impact of extrinsic (e.g., alcohol) and intrinsic (e.g., hormones) modulators on neurogenesis. Additionally, we provide an overview of the contribution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection to persistent neurological sequelae such as neurodegeneration, neurogenic defects and accelerated neuronal cell death. Together, our review provides a link between extrinsic/intrinsic factors and neurogenesis and explains the possible mechanisms of abnormal neurogenesis underlying neurological disorders.

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Oral exposure to high-dose ethanol for 28 days in rats reduces neural stem cells and immediate nascent neural progenitor cells as well as FOS-expressing newborn granule cells in adult hippocampal neurogenesis

Cited by 7 publications

References 57 publications

Effects of Genetics and Sex on Acute Gene Expression Changes in the Hippocampus Following Neonatal Ethanol Exposure in BXD Recombinant Inbred Mouse Strains

Effects of Genetics and Sex on Acute Gene Expression Changes in the Hippocampus Following Neonatal Ethanol Exposure in BXD Recombinant Inbred Mouse Strains

Development and experimental validation of an M2 macrophage and platelet‐associated gene signature to predict prognosis and immunotherapy sensitivity in bladder cancer

The Effects of Extrinsic and Intrinsic Factors on Neurogenesis

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