Oral delivery of live yeast Debaryomyces hansenii modulates the main innate immune parameters and the expression of immune-relevant genes in the gilthead seabream (Sparus aurata L.)

Reyes‐Becerril, Martha; Salinas, Irene; Cuesta, Alberto; Meseguer, José; Tovar‐Ramírez, Dariel; Ascencio‐Valle, Felipe

doi:10.1016/j.fsi.2008.02.010

Cited by 112 publications

(58 citation statements)

References 47 publications

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“…In previous studies, complement activity was undetected in the skin mucus of seabream [20,50], but it was reported in . At gene level, a c3 up-regulation was found after yeast dietary administration in gilthead specimens [51] whilst bathing of turbot with acid lactic probiotics also up-regulated its transcription in several tissues [52]. Taking this into account, our results at protein level of C3 over-expression in skin mucus of seabream after Pdp11 probiotic diet may indicate beneficial effects in the key component C3 in skin mucosal…”

Section: Effects Of Pdp11 And/or Overcrowding Stress On Immune-relatementioning

confidence: 58%

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Cordero¹,

Morcillo

Cuesta

et al. 2016

Journal of Proteomics

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Differential proteome profile of skin mucus of gilthead seabream (sparus aurata) after probiotic intake and/or overcrowding stress, Journal of Proteomics (2015Proteomics ( ), doi: 10.1016Proteomics ( /j.jprot.2015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E DM A N U S C R I P T ACCEPTED MANUSCRIPT A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT2 AbstractGilthead seabream (Sparus aurata L.) is the major cultured fish species in the Mediterranean area. High density stocking causes stress and increases the impact of diseases leading to economic losses. Probiotics could represent a solution to prevent diseases through several mechanisms such as improving the immune status and/or mucosal microbiota or competing with pathogens. The probiotic Shewanella putrefaciens, also known as Pdp11, was firstly isolated from the skin of healthy gilthead seabream. Our study focuses on the skin mucus proteome after dietary probiotic Pdp11 intake in fish maintained under normal or overcrowding conditions. 2-DE of skin mucus followed by LC-MS/MS analysis was done for each experimental group and differentially expressed proteins were identified. The results showed differentially expressed proteins especially involved in immune processes, such as lysozyme, complement C3, natural killer cell enhancing factor and nonspecific cytotoxic cell receptor protein 1, whose transcript profiles were studied by qPCR. A consistency between lysozyme protein levels in the mucus and lysozyme mRNA levels in skin were found. Further research is necessary to unravel the implications of skin mucosal immunity on fish welfare and disease. Biological significanceThe present work reveals the proteomic changes, which are taking place in the skin mucus of stressed and non-stressed gilthead seabream after Pdp11 probiotic intake. The study contributes to improve the knowledge on skin mucosal immunology of this relevant farmed fish species.Furthermore, the paper shows for the first time how a suitable proteomic methodology, in this case 2-DE followed by LC-MS/MS is useful to perform a comparative study with a noninvasive technique of skin mucus of gilthead seabream.

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Section: Effects Of Pdp11 And/or Overcrowding Stress On Immune-relatementioning

confidence: 58%

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Cordero¹,

Morcillo

Cuesta

et al. 2016

Journal of Proteomics

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“…The most studied are lactic acid bacteria (LAB) (Lactobacillus, Carnobacterium, Enterococcus, Lactococcus, Micrococcus, Streptococcus, and Weissella) (Balcazar et al, 2008;Balcázar et al, 2007;Hagi & Hoshino, 2009;Pérez-Sánchez et al, 2011;Vazquez et al, 2005;Villamil et al, 2002), Bacillus (Ai et al, 2011;Antony et al, 2011;Balcázar & Rojas-Luna, 2007;Bandyopadhyay & Das Mohapatra, 2009;Ochoa-Solano & Olmos-Soto, 2006;Liu et al, 2009;Nakayama et al, 2009;Newaj-Fyzul et al, 2007;Olmos et al, 2011;Salinas et al, 2005;Sun et al, 2010;Vaseeharan & Ramasamy, 2003), Vibrio (Fjellheim et al, 2007;Thompson et al, 2010), Pseudomonas (Abd El-Rhman et al, 2009;Chythanya, 2002;Das et al, 2006;Preetha et al, 2007;Ström-Bestor, Wiklund, 2011), and Aeromonas (Irianto et al, 2003;Lategan et al, 2006;. Yeasts (Saccharomyces, Debaryomyces) (Abdeltawwab et al, 2008;Reyes-Becerril et al, 2008;Tovar-Ramírez et al, 2010), bacterial spore formers (Hong et al, 2005) and recently Actinobacteria have also generated interest due to their high metabolic potential (Das et al, 2010;You et al, 2007). These probiotics have been used in different aquatic organisms, such as teleost fish (Merrifield et al, 2010;Dimitroglou et al, 2011), prawns (Van Hai et al, 2009, shrimp …”

Section: The Use Of Probiotics As An Alternative To Antibiotics In Aqmentioning

confidence: 99%

Antibiotics in Aquaculture – Use, Abuse and Alternatives

Romero¹,

Feijóo²,

Navarrete³

2012

Health and Environment in Aquaculture

308

325

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“…Other studies showed an increase in immune response with the use of probiotics for different species, such Carnobacterium maltaromaticum B26 and Carnobacterium divergens B33 for rainbow trout [38], Lactobacillus belbrüeckii, Bacillus subtilis and Debaryomyces hansenii for gilthead seabream [39][40][41], B. subtilis and Pseudomonas aeruginosa for Labeo rohita [42,43], Lactococcus lactis for Nile tilapia (Oreochromis niloticus) [44] and B. simplex DR-834 to carp (Cyprinus carpio) [45].…”

Section: Immune Systemmentioning

confidence: 99%