Oral Administration of Chitosan Attenuates Bleomycin-induced Pulmonary Fibrosis in Rats

Kim, Youseok; Li, Qiang; Youn, Hwa‐Young; Kim, Dae Young

doi:10.21873/invivo.11624

Cited by 12 publications

(11 citation statements)

References 24 publications

(18 reference statements)

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“…To prove this theory right, the immunohistochemical expression of TGF-β was assessed in histological sections of lungs with BLM-induced pulmonary fibrosis. As expected, untreated animals presented the greatest counts of TGF-β-expressing cells, which agrees with other studies previously reported [ 56 , 82 , 83 ]. The significant decrease in pulmonary cells expressing TGF-β in EOCW-treated groups at 100 and 200 mg/kg, as well as in the deflazacort-treated group, is fully supported by the analysis of α-SMA-positive cells, attesting to the close relation between this cytokine and myofibroblast differentiation.…”

Section: Discussionsupporting

confidence: 93%

“…Supporting our findings, acute alveolitis and interstitial inflammation, and consequent increased leukocyte recruitment, have been previously reported to occur after intratracheal administration of bleomycin in rodents [54]. Although the exchange between inflammation and fibrosis begins to occur between days 8 and 14, the presence of plasma exudation and, consequently, leukocytes in BALF, between 21 and 28 days has been reported [55,56], as observed in the current study. Hence, these data suggest the long-term persistence of the aggression and the inflammatory response in the lung tissues.…”

Section: Discussionsupporting

confidence: 92%

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Cymbopogon winterianus Essential Oil Attenuates Bleomycin-Induced Pulmonary Fibrosis in a Murine Model

et al. 2021

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The essential oil of Cymbopogon winterianus (EOCW) is a natural product with antioxidant, anti-inflammatory, and antifibrotic properties. We studied the effect of EOCW in the progression of histological changes of pulmonary fibrosis (PF) in a rodent model. The oil was obtained by hydrodistillation and characterized using gas chromatography–mass spectrometry. Intratracheal instillation of bleomycin was performed in 30 rats to induce PF, while Sham animals were subjected to instillation of saline solution. The treatment was performed using daily oral administration of distilled water, EOCW at 50, 100, and 200 mg/kg, and deflazacort (DFC). After 28 days, hemogram and bronchoalveolar lavage fluid (BALF), tissue levels of malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) were assayed. Histological grading of PF, immunohistochemical expression of α-smooth muscle actin (α-SMA), and transforming growth factor-β (TGF-β) were also analyzed. The EOCW major compounds were found to be citronellal, geraniol, and citronellol. EOCW significantly reduced inflammation in BALF, reduced MDA levels, and increased SOD activity. EOCW attenuated histological grading of PF and reduced immunohistochemical expression of α-SMA and TGF-β in a dose-dependent way, likely due to the reduction of oxidative stress, inflammation, and TGF-β-induced myofibroblast differentiation.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

Cymbopogon winterianus Essential Oil Attenuates Bleomycin-Induced Pulmonary Fibrosis in a Murine Model

et al. 2021

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“…Chitosan may induce quite different or more complex effects in in vivo. It has been recently reported that orally administered chitosan show antifibrotic effects in a rat model of bleomycin-induced pulmonary fibrosis (20). It should be tested whether such favorable effect may be induced by binding of chitosan to the oral tissue, thus inhibiting the production of pro-inflammatory cytokines.…”

Section: Discussionmentioning

confidence: 99%

Biological Properties of the Aggregated Form of Chitosan Magnetic Nanoparticle

Paulino-Gonzalez¹,

Sakagami²,

Bandow³

et al. 2020

In Vivo

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Background/Aim: Chitosan-coated iron oxide nanoparticles (Chi-NP) have gained attention because of their biocompatibility, biodegradability, low toxicity and targetability under magnetic field. In this study, we investigated various biological properties of Chi-NP. Materials and Methods: Chi-NP was prepared by mixing magnetic NP with chitosan FL-80. Particle size was determined by scanning and transmission electron microscopes, cell viability by MTT assay, cell cycle distribution by cell sorter, synergism with anticancer drugs by combination index, PGE 2 production in human gingival fibroblast was assayed by ELISA. Results: The synthetic process of Chi-NP from FL-80 and magnetic NP increased the affinity to cells, up to the level attained by nanofibers. Upon contact with the culture medium, Chi-NP instantly formed aggregates and interfered with intracellular uptake. Aggregated Chi-NP did not show cytotoxicity, synergism with anticancer drugs, induce apoptosis (accumulation of subG1 cell population), protect the cells from X-ray-induced damage, nor affected both basal and IL-1β-induced PGE 2 production. Conclusion: Chi-NP is biologically inert and shows high affinity to cells, further confirming its superiority as a scaffold for drug delivery.

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“…Other polysaccharides of interest include fucoidan, a sulphated polysaccharide found in brown seaweed [ 59 ], and chitosan, a linear polysaccharide with antibacterial, antifungal, antioxidant and anti-inflammatory activities. Both these polysaccharides have shown to exert anti-fibrotic effects when administrated orally, before and after lung damage [ 60 , 61 ]. Anti-fibrotic effects linked to EMT inhibition were also described by the administration of plant-derived polysaccharides, including those extracted from Dendrobium officionale [ 62 ] or from the ancient Chinese herbal formula Yupingfeng [ 63 ].…”

Section: The Role Of Nutritional Factors In Ipfmentioning

confidence: 99%

Insights into the Role of Bioactive Food Ingredients and the Microbiome in Idiopathic Pulmonary Fibrosis

Barceló

Joan

Río

et al. 2020

IJMS

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Idiopathic pulmonary fibrosis (IPF) is a chronic disease mainly associated with aging and, to date, its causes are still largely unknown. It has been shown that dietary habits can accelerate or delay the occurrence of aging-related diseases; however, their potential role in IPF development has been underestimated so far. The present review summarizes the evidence regarding the relationship between diet and IPF in humans, and in animal models of pulmonary fibrosis, in which we discuss the bioactivity of specific dietary food ingredients, including fatty acids, peptides, amino acids, carbohydrates, vitamins, minerals and phytochemicals. Interestingly, many animal studies reveal preventive and therapeutic effects of particular compounds. Furthermore, it has been recently suggested that the lung and gut microbiota could be involved in IPF, a relationship which may be linked to changes in immunological and inflammatory factors. Thus, all the evidence so far puts forward the idea that the gut-lung axis could be modulated by dietary factors, which in turn have an influence on IPF development. Overall, the data reviewed here support the notion of identifying food ingredients with potential benefits in IPF, with the ultimate aim of designing nutritional approaches as an adjuvant therapeutic strategy.

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Oral Administration of Chitosan Attenuates Bleomycin-induced Pulmonary Fibrosis in Rats

Cited by 12 publications

References 24 publications

Cymbopogon winterianus Essential Oil Attenuates Bleomycin-Induced Pulmonary Fibrosis in a Murine Model

Cymbopogon winterianus Essential Oil Attenuates Bleomycin-Induced Pulmonary Fibrosis in a Murine Model

Biological Properties of the Aggregated Form of Chitosan Magnetic Nanoparticle

Insights into the Role of Bioactive Food Ingredients and the Microbiome in Idiopathic Pulmonary Fibrosis

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