Oral Administration of Astragalus membranaceus Inhibits the Development of DNFB-Induced Dermatitis in NC/Nga Mice

Lee, Su Jin; Oh, Saeroonter; Seo, Sang Wan; Ahn, Hyung Joon; Geum, Dongho; Cho, Jeong Je; Park, Cheung Seog

doi:10.1248/bpb.30.1468

Cited by 44 publications

(38 citation statements)

References 18 publications

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“…Beside its immunostimulating activity, other studies have shown the direct antimicrobial activity of different extracts and pure compounds that derived from Astragalus species. In mice infected with coxsackie B-3 virus, Astragalus inhibited viral replication in the myocardial tissue while improving abnormal myocardial electric activity (Lee et al, 2007). The antimicrobial and antifungal properties of several crude extracts from different parts of other Astragalus species have been reported (Bisignano et al, 1994;El Sebakhy et al, 1994;Pistelli et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Anti-candidal activity of Astragalus verus in the in vitro and in vivo guinea pig models of cutaneous and systemic candidiasis

Mikaeili¹,

Karimi²,

Shamspur³

et al. 2012

Rev. bras. farmacogn.

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This study was design to evaluate the anti-candidal activity of Astragalus verus Olivier, Fabaceae (Av). The GC/MS analysis of essential oils of Av showed that aqueous extract contains thymol while hexadecanoic acid, 1,2-benzenedicarboxylic acid, diisooctyl ester and phytol were found as major components of methanol and acetone extracts. The aqueous extract showed anti-candidal activity in the concentration 320 mg/mL using disc diffusion method and its minimum inhibitory concentration (MIC) was 160 mg/mL. To induce cutaneous candidiasis, the dorsum of immunocompromised guinea pigs was infected with Candida albicans and animals were divided into five groups (n=5 for each): NC, received a vehicle; PC, received topical ketoconazole 2% and three other groups which received topical 10, 20 and 40% aqueous extract of Av. On ninth day postinfection, skins were cultured and colony forming unite per gram (CFU/g) skin was recorded. Systemic candidiasis was obtained by intravenous inoculation of C. albicans, 4000 CFU/g body weight. Here, animals have been divided into five groups like cutaneous candidiasis but their medications have been delivered in drinking water for ten days before induction of infection. On second day postinfection, all internal tissues were taken for determining CFU/g tissue. The aqueous extract (40%) prevented heavy burden of C. albicans in tissues and skin in oral and topical application, respectively. The results indicate that Av represents a potential source of anti-candidal drug.

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Section: Discussionmentioning

confidence: 99%

Anti-candidal activity of Astragalus verus in the in vitro and in vivo guinea pig models of cutaneous and systemic candidiasis

Mikaeili¹,

Karimi²,

Shamspur³

et al. 2012

Rev. bras. farmacogn.

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“…The study duration was 3 weeks, and dermatitis was induced by challenging with 0.15% DNFB twice a week throughout the study period. The D and S groups were treated with distilled and spring water twice a day at room temperature, respectively, and skin symptoms were evaluated as described by Lee et al 14) Briefly, three AD-like symptoms (dryness, crust, keratinization) were scored into four graded severities from 0 (none) to 3 (severe). Total serum IgE was measured using a mouse IgE measurement EIA kit (Yamasa Co., Chiba, Japan).…”

Section: Methodsmentioning

confidence: 99%

Influence of Hot Spring Water on Fatty Acid Composition of Skin Surface Lipids in Hairless Mouse Model of Atopic Dermatitis

Nishimura

Aoyanagi

Uchino

et al. 2016

Biological & Pharmaceutical Bulletin

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When hairless NCN24 mice with atopic dermatitis (AD) were sprayed with a petroleum-containing alkaline salt spring water rich in metaboric acid and sodium bicarbonate, AD symptoms diminished. Reversedphase HPLC with fluorescence detection (HPLC/FD) and online MS revealed that fatty acid (FA) composition of the skin surface lipids was similar to that in non-AD mice compared with that in AD mice. Strong negative correlations were noted between the levels of total serum immunoglobulin E (IgE) and palmitoleic acid and between the levels of total serum IgE and branched-hexadecanoic acid. Conversely, a strong positive correlation was noted between the levels of total serum IgE and linoleic acid. The present study demonstrates that the petroleum-containing spring water alters the FA composition of skin surface lipids in AD mice, which can be used as an index to evaluate inflammation.Key words atopic dermatitis; hot spring water; fatty acid; NCN24 mouse; skin surface lipid; fluorescence detection Atopic dermatitis (AD) is a common inflammatory skin disease that has both genetic and environmental factors in its etiology.1) It is well known that lipids, fatty acids (FAs) and their metabolites play an important role in the control of inflammation in AD.2) There are many reports on the relation between FA composition and inflammation. For example, an abnormal n-6 FA composition in cheek mucosal cells of children with AD 3) and an elevation of serum linoleic acid (18 : 2n-6) level in children with AD compared with that in non-AD children 4) have been observed in studies on AD and lipid metabolism. It has also been observed that arachidonic acid (20 : 4n-6) significantly accumulated in blood cells of NC/Nga mice (an AD animal model) with AD. 2)Using NC/Nga mice, 5,6) we had previously investigated methods for assessing balneotherapy effects on pathogenesis of AD, in which the dermatitis score was used as an index to evaluate inflammation. 7,8) Comprehensive analysis of gene expression in NC/Nga mice with AD revealed significant changes in expression of several genes involved in fatty acid metabolism, such as acetyl CoA carboxylase.8) To our knowledge, however, there have been no reports on FA composition of the skin surface lipids that play an important role in moisturizing and the skin barrier system in AD mouse models. This may be due to difficulty in lipid extraction from mice with hair such as NC/Nga mice.In the present study, we investigated the influence of spring water on the FA composition of skin surface lipids by using a hairless AD mouse model, NCN24 mice (transgenic mice) 9) and reversed-phase HPLC with fluorescence detection (HPLC/ FD).10,11) The spring water examined was available from Toyotomi hot spring (Hokkaido, Japan) that is famous for being effective against AD and psoriasis, and many people from across the country has visited the watering for cure. 12) One of the features of the spring is to include petroleum-containing hot spring water that is rare in Japan. Although there is not enough scientific evide...

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“…All mice were coded. To induce atopic dermatitislike skin lesions, a method described by Lee et al (2007) was followed.…”

Section: Methodsmentioning

confidence: 99%

A comparison of the leaf gel extracts of Aloe ferox and Aloe vera in the topical treatment of atopic dermatitis in Balb/c mice

2015

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Aloe vera gel is widely used in the treatment of an array of disturbances, especially skin disorders. The woundhealing effects have been attributed to its moisturiz-ing and anti-inflammatory effects as well as its beneficial effect on the maturation of collagen. The aim of the present study is to compare the effects of topically applied extracts of Aloe ferox with that of Aloe vera on the symptoms as well as IgE levels of a mouse model of atopic dermatitis (AD). Mice were sensitized and challenged with 2,4-dinitrochlorobenzene and treated afterwards for 10 consecutive days with the gels of either A. ferox or A. vera applied topically to the affected areas. A placebo gel was used for the control mice. Blood was collected at the beginning and end of the treatment period to measure serum IgE levels. Although the gels of both the Aloe species inhibited the cutaneous inflammatory response as well as serum IgE levels in the rats, the extracts of A. ferox were superior to that of A. vera in reducing IgE levels. The gels of A. ferox and A. vera, applied topically, may be a safe and useful alter-native to antihistamines and topical corticosteroids, for the treatment of patients suffering from recurring chronic AD.

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Oral Administration of Astragalus membranaceus Inhibits the Development of DNFB-Induced Dermatitis in NC/Nga Mice

Cited by 44 publications

References 18 publications

Anti-candidal activity of Astragalus verus in the in vitro and in vivo guinea pig models of cutaneous and systemic candidiasis

Anti-candidal activity of Astragalus verus in the in vitro and in vivo guinea pig models of cutaneous and systemic candidiasis

Influence of Hot Spring Water on Fatty Acid Composition of Skin Surface Lipids in Hairless Mouse Model of Atopic Dermatitis

A comparison of the leaf gel extracts of Aloe ferox and Aloe vera in the topical treatment of atopic dermatitis in Balb/c mice

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