Oral 2‐hydroxyoleic acid inhibits reflex hypersensitivity and open–field‐induced anxiety after spared nerve injury

Ávila-Martín, Gerardo; Galán-Arriero, Iriana; Ferrer-Donato, Águeda; Busquets, Xavier; Gómez-Soriano, Julio; Escribá, Pablo V.; Taylor, Julian

doi:10.1002/ejp.528

Cited by 23 publications

(15 citation statements)

References 69 publications

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“…In our previous work with the SCI model [2], no evidence for the development of anxiety measured with the open field test was observed, in line with another study [8]. In contrast the present study with the "spared nerve injury" model supports our previous observation that anxiety develops after peripheral nerve injury [18], suggesting the involvement of pathology-specific mechanisms that are more closely related to this comorbidity. In a previous study we suggested that the neural mechanisms that mediate apprehension are independent of those that mediate aversion measured with the PEAP test [2].…”

Section: Discussionsupporting

confidence: 79%

“…The rats were recorded and tracked for five minutes, and the software calculated the total time spent in each zone and the total distance moved. Anxiety behavior was confirmed when the animals demonstrated reduced exploration time within the inner zone also on day 21 after SNI [18,19].…”

Section: Methodsmentioning

confidence: 85%

“…In contrast when the animal moved to the white-walled chamber, the same filament was applied to non-injured contralateral paw. The stimulus was applied once every 15 s, for a total duration of 30 min [17,18]. The open field-induced anxiety test was also performed to evaluate the animalís behavior during the presentation of a novel situation at day 21 after SNI This test consisted of placing the animal in a 100 × 100 cm open field surrounded by a black curtain to exclude external cues, with a camera sited above the area connected to a commercial videotracking system (Ethovision XT software, Noldus Information Technology, Wageningen, Netherlands).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Early treatment with UR13870, a novel inhibitor of p38α mitogenous activated protein kinase, prevents hyperreflexia and anxiety behaviors, in the spared nerve injury model of neuropathic pain

Galán-Arriero¹,

Ávila-Martín²,

Ferrer-Donato³

et al. 2015

Neuroscience Letters

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Section: Discussionsupporting

confidence: 79%

Section: Methodsmentioning

confidence: 85%

Section: Methodsmentioning

confidence: 99%

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Early treatment with UR13870, a novel inhibitor of p38α mitogenous activated protein kinase, prevents hyperreflexia and anxiety behaviors, in the spared nerve injury model of neuropathic pain

Galán-Arriero¹,

Ávila-Martín²,

Ferrer-Donato³

et al. 2015

Neuroscience Letters

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“…Behavioural alterations have been described in rat models of peripheral traumatic nerve injury (Hu et al., 2007; Blackbeard et al., 2012; Galan‐Arriero et al., 2015; Wang et al., 2015; Avila‐Martin et al., 2015; Grégoire et al., 2012), neuropathic viral infection (Hasnie et al., 2007; Wallace et al., 2007a) and drug‐induced neuropathies (Wallace et al., 2007b; Huang et al., 2013). …”

Section: Introductionmentioning

confidence: 99%

Enhanced c‐Fos expression in the central amygdala correlates with increased thigmotaxis in rats with peripheral nerve injury

Morland

Novejarque

Spicer

et al. 2016

European Journal of Pain

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BackgroundPain is associated with affective, cognitive and sensory dysfunction. Animal models can be used to observe ethologically relevant behaviours such as thigmotaxis, giving insight into how ongoing sensory abnormalities influence natural rodent behaviours. The amygdala is a complex group of nuclei implicated in the integration and generation of emotional behavioural responses, including those associated with pain, and a region known as the central amygdala is particularly associated with generation of behavioural responses, due to its links to the descending pain modulation pathways; as such, study of amygdalar c‐Fos immunoreactivity can help identify the neuronal circuits involved.MethodThis study investigated changes in both nociceptive evoked responses and open field behaviour following spinal nerve transection (SNT) in male Wistar rats, and attempted to correlate these with changes in central amygdala c‐Fos immunoreactivity.ResultsFourteen days after SNT, mechanical hypersensitivity was present in the hind paw ipsilateral to site of injury. Thigmotactic behaviour was significantly increased in both SNT and sham surgery animals, with c‐Fos immunoreactivity in the central amygdala significantly greater in SNT animals compared to both sham and naive groups. Activation was greatest in the capsular and lateral subnuclei of the central amygdala, and in the caudal‐most regions. There was a strong correlation between thigmotactic behaviour and central amygdala activation following SNT surgery not seen in sham animals suggesting a role for the amygdala in behavioural responses to peripheral nerve injury.ConclusionsThis study provides evidence to support the role of the amygdala in thigmotactic open field behaviour following SNT.What does this study add?Thigmotaxis and amygdala activation are positively correlated in rats following spinal nerve transection.Behavioural changes seen in sham animals did not correlate with amygdala activation, suggesting amygdala activation is related to nociceptive input.Evoked measures, such as hindpaw withdrawal, are not correlated with either thigmotaxis or amygdala activation, emphasizing the importance of complex behaviours when studying pain.

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“…Mechanical reflex hypersensitivity (Avila-Martin et al, 2015) was recorded at specific time-points after SCI by two independent examiners blinded to the experimental conditions. The rats were placed in a plastic box that was placed on a wire mesh platform.…”

Section: Behavioral Testsmentioning

confidence: 99%

Meta-analysis of stem cell transplantation for reflex hypersensitivity after spinal cord injury

Xue

Song

et al. 2017

Neuroscience

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Stem cells have been used in novel therapeutic strategies for spinal cord injury (SCI), but the effect of stem cell transplantation on neuropathic pain after SCI is unclear. The current meta-analysis evaluates the effects of stem cell transplantation on neuropathic pain after SCI. We first conducted online searches of PubMed, Web of Science, China Academic Journals Full-text Database, and Wanfang Data for randomized controlled trials that compared stem cell transplantation and vehicle treatments in rodent models of neuropathic pain after SCI. Quality assessment was performed using Cochrane Reviewer’s Handbook 5.1.0, and meta-analysis was conducted with RevMan 5.3. Then, we developed a rat model of SCI and transplanted bone marrow mesenchymal stem cells to verify meta-analysis results. Twelve randomized, controlled trials (n = 354 total animals) were included in our meta-analysis and divided by subgroups, including species, timing of behavioral measurements, and transplantation time after SCI. Subgroup analysis of these 12 studies indicated that stem cell-treated animals had a higher mechanical reflex threshold than vehicle groups, with a significant difference in both rats and mice. The thermal withdrawal latency showed the same results in mouse subgroups, but not in rat subgroups. In addition, mesenchymal stem cell transplantation was an effective treatment for mechanical, but not thermal reflex hypersensitivity relief in rats. Transplantation showed a positive effect when carried out at 3 or 7 days post-SCI. Stem cell transplantation alleviates mechanical reflex hypersensitivity in rats and mice and thermal reflex hypersensitivity in mice after SCI.

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Oral 2‐hydroxyoleic acid inhibits reflex hypersensitivity and open–field‐induced anxiety after spared nerve injury

Abstract: Oral administration of the modified omega 9 fatty acid, 2-OHOA, mediates antinociception and prevents open-field-induced anxiety in the SNI model in Wistar rats, which is mediated by an inhibition of spinal dorsal horn microglia activation.

Cited by 23 publications

References 69 publications

Early treatment with UR13870, a novel inhibitor of p38α mitogenous activated protein kinase, prevents hyperreflexia and anxiety behaviors, in the spared nerve injury model of neuropathic pain

Early treatment with UR13870, a novel inhibitor of p38α mitogenous activated protein kinase, prevents hyperreflexia and anxiety behaviors, in the spared nerve injury model of neuropathic pain

Enhanced c‐Fos expression in the central amygdala correlates with increased thigmotaxis in rats with peripheral nerve injury

Meta-analysis of stem cell transplantation for reflex hypersensitivity after spinal cord injury

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