Optimum dose of chitin and chitosan for organization of non-woven fabric in the subcutaneous tissue

Kojima, Kazuyuki; Okamoto, Yoshiharu; Miyatake, K; Tamai, Yoshin; Shigemasa, Yoshihiro; Minami, Saburo

doi:10.1016/s0144-8617(00)00326-x

Cited by 21 publications

(11 citation statements)

References 16 publications

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“…Chitin and chitosan having a mean particle size of 3.5 µm were sterilized separately using ethylene oxide gas and suspended in phosphate buffered solution (PBS, pH 7.2) at a concentration of 10 mg/ml. According to our measurements in a previous investigation [5], the optimum doses of chitin and chitosan in granulation tissue formation in rats were 1.0 and 10 mg/ml, and 0.1 and 1.0 mg/ml, respectively. In the chitin and chitosan at the higher concentrations, excessive inflammatory reactions occurred.…”

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confidence: 63%

“…In the field of medicine, the effects of chitin and chitosan on wound healing have been the focus of great attention since Prudden et al published a paper in 1970 stating that these agents accelerate wound healing [15]. Numerous reports describe the stimulatory effects of chitin and chitosan on tissue reactions involved in wound healing [1,[3][4][5][7][8][9][10][11][12][13]. Their most notable property is their propensity to induce abundant granulation tissue formation with angiogenesis.…”

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confidence: 99%

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Effects of Chitin and Chitosan on Collagen Synthesis in Wound Healing

et al. 2004

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ABSTRACT. Collagen synthesis was evaluated by measuring prolyl hydroxylase (PHL) activity induced within rat granulation tissue by a polyester non-woven fabric (NWF, 1 × 1 cm, 0.6 mm in thickness) impregnated with a chitin or chitosan suspension ranging in concentration from 0.1 to 10 mg/ml. In addition, PHL activity induced in rat granulation tissue by a NWF impregnated with a phosphate buffer solution was examined as a control. The PHL activity in each group remained low until 4 days post-implantation (Day 4). However, in the 10 mg chitin group, the PHL activity increased rapidly without scatter of the data at Day 7 and remained at a plateau until Day 14. In other groups, PHL activity increased linearly until Day 14. The data varied widely at Day 7. Compared to chitosan, chitin at the higher concentration was found to induce stable collagen synthesis in the early wound healing process. KEY WORDS: chitin, chitosan, prolyl hydroxylase.J. Vet. Med. Sci. 66(12): 1595-1598, 2004 Chitin and chitosan have been widely studied in both engineering and medicine. In the field of medicine, the effects of chitin and chitosan on wound healing have been the focus of great attention since Prudden et al. published a paper in 1970 stating that these agents accelerate wound healing [15]. Numerous reports describe the stimulatory effects of chitin and chitosan on tissue reactions involved in wound healing [1,[3][4][5][7][8][9][10][11][12][13]. Their most notable property is their propensity to induce abundant granulation tissue formation with angiogenesis. Previous reports indicated that chitin and chitosan induced fine collagen fibers histologically [3,11]. Moreover, chitin was found to enhance synthesis of types I, III, and IV collagen [4]. However, these reports indicate only the properties of the collagen synthesized. To understand more about wound healing acceleration by chitin and chitosan, it is important to clarify the quantity of collagen synthesized as well as its properties. In this paper, we evaluated the degree to which chitin and chitosan stimulate collagen synthesis by measuring prolyl hydroxylase activity, an activity necessary for collagen synthesis in rats [18].Thirty Wistar rats (adult females, weighing 270-300 g) were used in this study. The animals were purchased from Hamaguti Laboratory Co., Ltd. (Kobe, Japan). Chitin and chitosan were supplied by Sunfive Co., Ltd. (Tottori, Japan). Molecular weights and rates of deacetylation were determined using the viscoanetric method [19] and infra-red spectroscopic method [17], respectively. The data obtained for chitin and chitosan yielded molecular weights of 300 and 80 kD, and deacetylation rates of <10 and >80%, respectively. Chitin and chitosan having a mean particle size of 3.5 µm were sterilized separately using ethylene oxide gas and suspended in phosphate buffered solution (PBS, pH 7.2) at a concentration of 10 mg/ml. According to our measurements in a previous investigation [5], the optimum doses of chitin and chitosan in granulation tissue formation in rats w...

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confidence: 63%

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confidence: 99%

Effects of Chitin and Chitosan on Collagen Synthesis in Wound Healing

et al. 2004

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“…One of the interesting characteristics of wound healing with chitin and chitosan is the presence of macrophages in granulation tissue. [1,5,7,8] This phenomenon was notable in the granulation tissue induced by chitin. Macrophages are important cell components for the wound healing process, [13] because they release cytokines including TGF-b, PDGF, FGF, TNF-a, IL-1 and IL-2, which are involved in the wound healing process.…”

Section: Introductionmentioning

confidence: 94%

“…We have investigated some of the mechanisms of wound healing acceleration induced by chitin and chitosan in vivo [1][2][3][4][5][6][7][8] and in vitro. [9][10][11][12] The mechanisms are not yet completely elucidated.…”

Section: Introductionmentioning

confidence: 99%

Effects of Chitin/Chitosan and Their Oligomers/Monomers on Migrations of Macrophages

Okamoto

Inoue

Miyatake

et al. 2003

Macromolecular Bioscience

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The effects of chitin/chitosan and their oligomers/monomers on the migrations of mouse peritoneal macrophage (PEM) and the rat macrophage cell line (rMp) were evaluated in vitro. In direct migratory assay using the blind well chamber method, the migratory activity of PEM was enhanced significantly by chitin and chitosan oligomers (NACOS and COS, respectively), but reduced by chitin, chitosan, and the chitosan monomer (GlcN). The migratory activity of rMp was increased significantly by chitin, chitosan, and the polymers, NACOS and GlcN.

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“…However, the HRs in the tested conditions were all below 2.0 %, indicating the suitability of OA-g-CSO nanoparticles for intravenous administration. Moreover, since chitosan is reported to exhibit dosedependent blood compatibility and cell viability [33], it should be emphasized that only the requisite concentration of OA-g-CSO nanoparticles be used in applications to avoid additional hemolysis.…”

Section: Discussionmentioning

confidence: 99%

Self-assembled polymeric nanoparticles based on oleic acid-grafted chitosan oligosaccharide: biocompatibility, protein adsorption and cellular uptake

Chen

Huang

Han

et al. 2012

J Mater Sci: Mater Med

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Oleic acid-grafted chitosan oligosaccharide (OA-g-CSO) was synthesized to prepare self-assembled nanoparticles by sonication at physiological pH value (7.4). The nanoparticles appeared to be spherical in shape with a diameter of 158.1 ± 64.3 nm. The biocompatibility of OA-g-CSO nanoparticles was evaluated in vitro via MTT assay and hemolysis test. The nanoparticles showed no cytotoxicity to mouse embryo fibroblasts and the hemolysis rates came well within permissible limits (<2 %) in the tested conditions. When incubated with bovine calf serum, the protein adsorption on the surface of OA-g-CSO nanoparticles was concentration-dependent, and the amount of bovine serum albumin was in the highest proportion of the total amount of adsorbed proteins. Cellular uptake rate was evaluated by incubating fluorescence labeled OA-g-CSO nanoparticles with human lung carcinoma cells (A549). OA-g-CSO nanoparticles could be taken up by A549 cells, and the uptake rates increased with incubation time and particle concentration.

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Optimum dose of chitin and chitosan for organization of non-woven fabric in the subcutaneous tissue

Cited by 21 publications

References 16 publications

Effects of Chitin and Chitosan on Collagen Synthesis in Wound Healing

Effects of Chitin and Chitosan on Collagen Synthesis in Wound Healing

Effects of Chitin/Chitosan and Their Oligomers/Monomers on Migrations of Macrophages

Self-assembled polymeric nanoparticles based on oleic acid-grafted chitosan oligosaccharide: biocompatibility, protein adsorption and cellular uptake

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