Optimizing the Metabolic Performance of Mixed
Bacterial Culture Towards Dibenzothiophene
Desulfurization under the Effect of Varying
Nutrient and Environmental Factors

Microscopy Res & Technique

Ali

Jamal

et al. 2022

Self Cite

Excessive emission of sulfur dioxides from the combustion of coal and other fossil fuels for thermal and industrial purposes has been associated with serious environmental hazards. Biodesulfisation (BDS) can be an effective approach for reducing the impact of toxic gases to its inbuilt operational feasibility under ambient environmental conditions. In the present research, two strategies for BDS of a standard organosulfur compound such as dibenzothiophene (DBT) were investigated under laboratory conditions. In the first treatment, the role of different surfactants such as Tween-20, Tween-80, SDS, and EDTA on the desulfurization of DBT was investigated by the application of bacterial consortium IQMJ-5. In the second treatment, Iron oxidenanoparticles were synthesized and immobilized on the surface of bacteria cells. Shake flask experiments were conducted with immobilized cells, surfactant amended immobilized cells, and control or noncoated cells. Among different surfactant treatments, Tween-80 was found to be the most effective surfactant, showing maximum desulfurization activity at a concentration of 5 g/L. The transmission electron microscopy and X-ray diffraction analysis indicated that produced nanoparticles were spherical in shape with a size of about 46 nm and had a stoichiometric ratio of 55.85% and 44.15% between O and Fe, respectively. The nanoparticle treatment enhanced the DBT desulfurization process up to 11.37% as compared to the control, specifically when immobilized cells were used. Therefore, it was concluded that nanoparticles treatments with immobilization of the bacterial cells enhanced the desulfurization rate of DBT under ambient reaction conditions and provide a sustainable alternative for commercial coal BDS.

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Response of mixed bacterial culture towards dibenzothiophene desulfurization under the influence of surfactants and microscopically (SEM and TEM) characterized magnetic Fe₃O₄ nanoparticles

Microscopy Res & Technique

Ali

Jamal

et al. 2022

Self Cite

“…However, the combustion of an excessive volume of sulfur-containing coal is responsible for an increased discharge of harmful or toxic gases, especially SO 2 (Xu et al, 2019). Such sulfur-containing toxic gases after release can cause health issues, acid rain, and other environmental problem including air pollution (Khan et al, 2022a).…”

Section: Introductionmentioning

confidence: 99%

Assessment of the dibenzothiophene desulfurization potential of indigenously isolated bacterial consortium IQMJ-5: a different approach to safeguard the environment

Ali²,

Jamal³

et al. 2023

Arch Microbiol

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Biodesulfurization is emerging as a valuable technology for the desulfurization of dibenzothiophene (DBT) and its alkylated substitutes, which are otherwise regarded as refractory to other physical and chemical desulfurizing techniques. However, the technique is currently facing the issue of the nonavailability of an effective microbial isolate with the capability of increased desulfurizing rate and the ability to tackle the problem of product inhibition. Pure cultures belonging to different genera have been used at a large scale for the desulfurization of fossil fuels while studies on the desulfurization of fossil fuels employing microbial consortia are entirely missing. Herein, we isolated several kinds of dibenzothiophene desulfurizing bacterial consortia from hydrocarbon-contaminated soil samples by conventional enrichment technique. The outcomes of Gibb's assay analysis showed that six isolates followed the "4S" pathway and converted DBT to 2-hydroxybiphenyl (2-HBP). Among the isolates, I5showed maximum growth rate (0.0064 g dry cell weight L -1 h -1 ) and desulfurization activity (about 77% as indicated by HPLC analysis) and was considered for further in-depth experimentation. The analysis of 16S rRNA by high throughput sequencing approach of the I5 isolate revealed ve types of bacterial phyla including Proteobacteria, Bacteroidetes, Firmicutes, Patascibacteria, and Actinobacteria (in order of abundance). The isolate showed signi cant tolerance to the inhibitory effect of both 2-HBP and sulfate and maintained growth in the presence of even about 1.0 mM initial concentration of both products. This clearly suggests that the isolate can be an e cient candidate for improving the quality of the coal. HighlightsIsolation of dibenzothiophene desulfurizing bacterial consortia from hydrocarbon-contaminated soil samples The outcomes of Gibb's assay analysis showed that six isolates followed the "4S" pathway and converted DBT to 2-hydroxybiphenyl (2-HBP) I5 showed maximum growth rate (0.0064 g dry cell weight L -1 h -1 ) and desulfurization activityThe isolate showed signi cant tolerance to the inhibitory effect

“…Previously, our research team isolated a DBT desulfurizing bacterial consortium IQMJ-5 and optimized its environmental parameters (Khan et al, 2022a). Further, the consortium was immobilized with magnetic iron oxide (Fe 3 O 4 ) nanoparticles and has exhibited promising results (Khan et al, 2022b).…”

Section: Introductionmentioning

confidence: 99%

Effect of Fe3O4 coated bacterial consortium IQMJ-5 on the desulfurization of the organosulfur portion of Pakistani coal and its chemical characteristics

Ali

Achakzai³

et al. 2023

Preprint

Self Cite

Coal is an abundant and inexpensive source of energy with immense application in different industrial sectors. However, its extensive utilization has resulted in several kinds of sulfur-related environmental issues. To tackle such issues, indigenously isolated Fe3O4-coated bacterial consortium IQMJ-5 cells were applied for the desulfurization of a Pakistani coal. The coated cells were allowed to desulfurize the coal in a shake flask experiment. To assess the desulfurization capabilities, both the pre- and post-desulfurized coal were characterized by different chemical and analytical techniques. The total sulfur content identified in the coal sample was about 4.50%. The results of the analysis showed that after treatment an increase in the carbon content and heating value of the coal sample was detected. Moreover, an amount of about 54.46% and 66.6% of organic and total sulfur, respectively were removed after biodesulfurization. The obtained results clearly suggest that the consortium can be a potential candidate for reducing sulfur-related environmental pollutants of fossil fuels at a more advanced commercial scale.