Response of mixed bacterial culture towards dibenzothiophene desulfurization under the influence of surfactants and microscopically (<scp>SEM</scp> and <scp>TEM</scp>) characterized magnetic <scp>Fe<sub>3</sub>O<sub>4</sub></scp> nanoparticles

Ali

Achakzai³

et al. 2023

Preprint

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Coal is an abundant and inexpensive source of energy with immense application in different industrial sectors. However, its extensive utilization has resulted in several kinds of sulfur-related environmental issues. To tackle such issues, indigenously isolated Fe3O4-coated bacterial consortium IQMJ-5 cells were applied for the desulfurization of a Pakistani coal. The coated cells were allowed to desulfurize the coal in a shake flask experiment. To assess the desulfurization capabilities, both the pre- and post-desulfurized coal were characterized by different chemical and analytical techniques. The total sulfur content identified in the coal sample was about 4.50%. The results of the analysis showed that after treatment an increase in the carbon content and heating value of the coal sample was detected. Moreover, an amount of about 54.46% and 66.6% of organic and total sulfur, respectively were removed after biodesulfurization. The obtained results clearly suggest that the consortium can be a potential candidate for reducing sulfur-related environmental pollutants of fossil fuels at a more advanced commercial scale.

Section: Introductionmentioning

confidence: 99%

Effect of Fe3O4 coated bacterial consortium IQMJ-5 on the desulfurization of the organosulfur portion of Pakistani coal and its chemical characteristics

Ali

Achakzai³

et al. 2023

Preprint

Self Cite

Assessment of the dibenzothiophene desulfurization potential of indigenously isolated bacterial consortium IQMJ-5: a different approach to safeguard the environment

Ali²,

Jamal³

et al. 2023

Arch Microbiol

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Biodesulfurization is emerging as a valuable technology for the desulfurization of dibenzothiophene (DBT) and its alkylated substitutes, which are otherwise regarded as refractory to other physical and chemical desulfurizing techniques. However, the technique is currently facing the issue of the nonavailability of an effective microbial isolate with the capability of increased desulfurizing rate and the ability to tackle the problem of product inhibition. Pure cultures belonging to different genera have been used at a large scale for the desulfurization of fossil fuels while studies on the desulfurization of fossil fuels employing microbial consortia are entirely missing. Herein, we isolated several kinds of dibenzothiophene desulfurizing bacterial consortia from hydrocarbon-contaminated soil samples by conventional enrichment technique. The outcomes of Gibb's assay analysis showed that six isolates followed the "4S" pathway and converted DBT to 2-hydroxybiphenyl (2-HBP). Among the isolates, I5showed maximum growth rate (0.0064 g dry cell weight L -1 h -1 ) and desulfurization activity (about 77% as indicated by HPLC analysis) and was considered for further in-depth experimentation. The analysis of 16S rRNA by high throughput sequencing approach of the I5 isolate revealed ve types of bacterial phyla including Proteobacteria, Bacteroidetes, Firmicutes, Patascibacteria, and Actinobacteria (in order of abundance). The isolate showed signi cant tolerance to the inhibitory effect of both 2-HBP and sulfate and maintained growth in the presence of even about 1.0 mM initial concentration of both products. This clearly suggests that the isolate can be an e cient candidate for improving the quality of the coal. HighlightsIsolation of dibenzothiophene desulfurizing bacterial consortia from hydrocarbon-contaminated soil samples The outcomes of Gibb's assay analysis showed that six isolates followed the "4S" pathway and converted DBT to 2-hydroxybiphenyl (2-HBP) I5 showed maximum growth rate (0.0064 g dry cell weight L -1 h -1 ) and desulfurization activityThe isolate showed signi cant tolerance to the inhibitory effect

Assessment of the dibenzothiophene desulfurization potential of indigenously isolated bacterial consortium IQMJ-5: A different approach to safeguard the environment

Ali

Jamal

et al. 2022

Preprint

Self Cite

Biodesulfurization is emerging as a valuable technology for the desulfurization of dibenzothiophene (DBT) and its alkylated substitutes, which are otherwise regarded as refractory to other physical and chemical desulfurizing techniques. However, the technique is currently facing the issue of the nonavailability of an effective microbial isolate with the capability of increased desulfurizing rate and the ability to tackle the problem of product inhibition. Pure cultures belonging to different genera have been used at a large scale for the desulfurization of fossil fuels while studies on the desulfurization of fossil fuels employing microbial consortia are entirely missing. Herein, we isolated several kinds of dibenzothiophene desulfurizing bacterial consortia from hydrocarbon-contaminated soil samples by conventional enrichment technique. The outcomes of Gibb’s assay analysis showed that six isolates followed the “4S” pathway and converted DBT to 2-hydroxybiphenyl (2-HBP). Among the isolates, I5 showed maximum growth rate (0.0064 g dry cell weight L-1h-1) and desulfurization activity (about 77% as indicated by HPLC analysis) and was considered for further in-depth experimentation. The analysis of 16S rRNA by high throughput sequencing approach of the I5 isolate revealed five types of bacterial phyla including Proteobacteria, Bacteroidetes, Firmicutes, Patascibacteria, and Actinobacteria (in order of abundance). The isolate showed significant tolerance to the inhibitory effect of both 2-HBP and sulfate and maintained growth in the presence of even about 1.0 mM initial concentration of both products. This clearly suggests that the isolate can be an efficient candidate for improving the quality of the coal.