Optimizing optical coherence tomography and histopathology correlation in retinal imaging

Neto, Carlos A. Moreira; Bergeron, Sabrina; Coblentz, Jacqueline; Zoroquiaín, Pablo; Maloney, Shawn C.; Mastromonaco, Christina; Espósito, Evangelina; Romanò, A; Belfort, Rubens; Moreira, Carlos Augusto; Burnier, Julia V.; Burnier, Miguel N

doi:10.1016/j.jcjo.2018.06.024

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…Optical coherence tomography (OCT), a non-invasive technique with a high resolution, enables to describe retinal structures almost at a cellular level in vivo. OCT layers correlate well with histological findings of the retina [ 1 , 2 ]. The use of this imaging technique to demonstrate neurodegeneration of the retina or the optic disc in neurological and neuro-ophthalmological conditions is growing [ 3 ].…”

Section: Introductionmentioning

confidence: 72%

Retinal thinning in phenylketonuria and Gaucher disease type 3

Hopf

Schuster

Hennermann

et al. 2021

Graefes Arch Clin Exp Ophthalmol

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Purpose Retinal alterations in inherited metabolic diseases associated with neurodegeneration are poorly studied. The objective was to study retinal thickness, specifically the components of the ganglion cell complex (GCC)—nerve fiber layer (NFL), ganglion cell layer (GCL), and inner plexiform layer (IPL)—using spectral-domain optical coherence tomography (SD-OCT) in two different diseases with potential dopaminergic depletion, phenylketonuria (PKU) and Gaucher disease type 3 (GD3). Methods Retinal layers in 19 patients with PKU, 15 patients with GD3, and 93 healthy individuals were measured using peripapillary ring scan and macular SD-OCT. Linear mixed models were computed including an adjustment for age, sex, and spherical equivalent. We calculated Spearman’s rank correlations between retinal layer measurements and clinical and/or laboratory parameters. Results Thinning of total retinal thickness was found in the macular inner ring (p = 0.002), and outer ring (p = 0.012), sparing the fovea (p = 0.12) in PKU, while in GD3, all subfields were thinned (fovea p < 0.001, inner ring p = 0.047, outer ring 0.07). In both conditions, thinning was most evident in the NFL, GCL, and IPL, while OPL (outer plexiform layer) was thickened. Peripapillary retinal nerve fiber layer measurements remained normal. GCL and IPL in PKU correlated with tyrosine serum concentration. Conclusion Thinning of the NFL, GCL, and IPL, with thickened OPL, are both found in PKU and in GD3. Low dopamine concentrations in the retina might promote these effects. However, these data do not give evidence that retinal measurements can be used as a biomarker for disease severity in patients with GD3.

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Section: Introductionmentioning

confidence: 72%

Retinal thinning in phenylketonuria and Gaucher disease type 3

Hopf

Schuster

Hennermann

et al. 2021

Graefes Arch Clin Exp Ophthalmol

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“…The basis for the histologic processing-associated non-linear tissue shrinkage as difference between the retinal tissue shrinkage and the choroidal tissue shrinkage may be due to differences in the composition of the tissues. Histopathological studies have shown that the shrinkage in the intercellular space is less marked than that in the intracellular compartment, with the intracellular shrinkage occurring mainly in the cytoplasm rather than in the cell nucleus ( 24 ). The shrinkage of compact tissue such as the retina with a relatively high nucleus-to-cytoplasm ratio may thus be less marked than the shrinkage of the choroid consisting predominantly of loose connective tissue with higher fluid content.…”

Section: Discussionmentioning

confidence: 99%

In vivo Imaging of Retina and Choroid in Guinea Pigs

Dong

Jiang

et al. 2021

Front. Med.

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Purpose: To evaluate the feasibility of in-vivo imaging of the retina and choroid using spectral domain optical coherence tomography (OCT) in guinea pigs.Methods: The study included 19 pigmented guinea pigs (age: 3–4 weeks) which underwent sonographic axial length measurements and OCT imaging. At study end, the animals were sacrificed and histomorphometric examinations of the retina and choroid were performed. We assessed the reproducibility of the OCT measurements and compared in-vivo measurements to histomorphometric data.Results: The mean thickness of the retina and choroid near the optic nerve head was 175.6 ± 25.8 and 63.4 ± 16.5 μm, respectively, and mean Bruch's membrane opening (BMO) diameter was 831 ± 121 μm. The intra-observer comparison of measurements of retinal thickness (intraclass correlation coefficient (ICC) = 0.92, 95% CI: 0.86–0.96; P < 0.001), choroidal thickness (ICC = 0.92, 95% CI: 0.86–0.96; P < 0.001), and BMO diameter (ICC = 0.92, 95% CI: 0.86–0.96; P < 0.001) showed a high correlation. A high agreement was present also for the inter-observer reproducibility of the measurements of retinal thickness (Pearson correlation coefficient (R) = 0.98; P < 0.001), choroidal thickness (R = 0.96; P < 0.001), and BMO diameter (R = 0.98; P < 0.001). The Bland-Altman plots showed that 2.6% (1/38), 5.3% (2/38), and 7.9% (3/38) of the measurement points of retinal thickness, choroidal thickness and BMO diameter, respectively, were located outside of the 95% limits of agreement. The OCT-based thickness measurements of retina and choroid were significantly higher than those measured by histomorphometry (both P-values <0.01).Conclusion: OCT-based in-vivo morphometric imaging of the retina and choroid in guinea pigs is feasible with an acceptable intra-observer repeatability and inter-observer reproducibility.

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“…The available technologies are increasingly powerful, yet not fully exploited. Retina specialists use OCT imaging to visualize retinal layers in such details and in an orientation that is comparable to light or electron microscopy [1][2][3]. Our aim is to expand the use of OCT imaging to characterize peri-ocular skin cancers.…”

Section: Introductionmentioning

confidence: 99%

Optical Coherence Tomography of Peri-Ocular Skin Cancers: An Optical Biopsy

et al. 2020

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Introduction: Optical coherence tomography (OCT) imaging has been used as a diagnostic tool for retinal disease for several years, and OCT apparatuses are becoming increasingly powerful. However, OCT has yet to reach its full potential in ophthalmology clinics. Alike retinal layers, it has been shown that OCT is able to generate cross-sectional images of the skin and allows visualization of skin lesions in a histopathology-like manner. Objective: We aim to validate OCT as an imaging modality for peri-ocular skin cancer. Through a series of cases, we highlight findings for 3 common eyelid malignancies: basal cell carcinoma, squamous cell carcinoma and sebaceous carcinoma. We propose an OCT image-based signature for basal cell carcinoma. Methods: This is a prospective study. Fifty-eight lesions suspicious of malignancy from 57 patients were subjected to OCT imaging prior to the surgical excision of the lesion. OCT images were analysed and scored according to previously identified OCT features. Eight representative examples are presented, highlighting the OCT patterns for each malignancy side by side to its corresponding histopathological sections. Results: Out of the 58 lesions analysed, 53 were malignant. A loss of the dermal-epidermal junction is observed in all malignant lesions. A strong link is observed between the presence of subepithelial hyporeflective nests on OCT and the diagnosis of basal cell carcinoma (present in 83% of cases). Conversely, lesions of epithelial origin such as squamous cell carcinoma are most often represented on OCT by acanthosis. Two supplementary cases, one basal cell carcinoma and one sebaceous carcinoma, are provided to illustrate how OCT imaging is a valuable tool in cases where clinical observations may be unusual. Conclusions: We provide evidence supporting the use of OCT for the evaluation of peri-ocular cancers. OCT enables visualization of the skin layers in vivo, before biopsy. Our results show that certain OCT features can contribute to include or exclude a diagnosis of basal cell carcinoma. By integrating this non-invasive imaging methodology into the routine assessment of peri-ocular skin lesions, especially in health care centres where access to specialists is limited, OCT imaging can increase clinical precision, reduce delays in patient referral and enhance patient care.

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Optimizing optical coherence tomography and histopathology correlation in retinal imaging

Cited by 6 publications

References 16 publications

Retinal thinning in phenylketonuria and Gaucher disease type 3

Retinal thinning in phenylketonuria and Gaucher disease type 3

In vivo Imaging of Retina and Choroid in Guinea Pigs

Optical Coherence Tomography of Peri-Ocular Skin Cancers: An Optical Biopsy

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