Optimizing light regimes on growth and lipid accumulation in Ankistrodesmus fusiformis H1 for biodiesel production

He, Qiaoning; Yang, Haijian; Hu, Chunxiang

doi:10.1016/j.biortech.2015.09.085

Cited by 25 publications

(5 citation statements)

References 34 publications

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“…tertiolecta treated with 150 ppm piperonyl butoxide and 8000 Lux was the highest after cultivation for 96 h. Under this condition, the final oil accumulation and the lipid content of single cells increased by 21.79% and 76.42%, respectively, compared with those in the group treated with 0 ppm piperonyl butoxide at 8000 Lux. This may be because the light intensity of 6000 Lux increased photosynthetic activity and was more conducive to the accumulation of pigments, resulting in less carbon allocation in lipid . From another aspect, in high light intensity, dissipation of surplus photon energy and deterrence of photochemical destruction to algal cells could have been aided by ATP and NAD(P)H (produced by photosynthesis) in high light intensity, which are required in large amounts for the synthesis of lipid .…”

Section: Resultssupporting

confidence: 89%

“…This may be because the light intensity of 6000 Lux increased photosynthetic activity and was more conducive to the accumulation of pigments, resulting in less carbon allocation in lipid. 43 From another aspect, in high light intensity, dissipation of surplus photon energy and deterrence of photochemical destruction to algal cells could have been aided by ATP and NAD(P)H (produced by photosynthesis) in high light intensity, which are required in large amounts for the synthesis of lipid. 44 In Chlorella sp.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Effects of Piperonyl Butoxide on the Accumulation of Lipid and the Transcript Levels of DtMFPα in Dunaliella tertiolecta

Dai

Song

Chen

et al. 2022

J. Agric. Food Chem.

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As one of the sources of biodiesel, microalgae are expected to solve petroleum shortage. In this study, different concentrations of piperonyl butoxide were added to the culture medium to investigate their effects on the growth, pigment content, lipid accumulation, and content of carotenoids in Dunaliella tertiolecta. The results showed that piperonyl butoxide addition significantly decreased the biomass, chlorophyll content, and total carotenoid content but hugely increased the lipid accumulation. With the treatment of 150 ppm piperonyl butoxide combined with 8000 Lux light intensity, the final lipid accumulation and single-cell lipid content were further increased by 21.79 and 76.42% compared to those of the control, respectively. The lipid accumulation in D. tertiolecta is probably related to the increased expression of DtMFPα in D. tertiolecta under the action of piperonyl butoxide. The phylogenetic trees of D. tertiolecta and other oil-rich plants were constructed by multiple sequence alignment of DtMFPα, demonstrating their evolutionary relationship, and the tertiary structure of DtMFPα was predicted. In conclusion, piperonyl butoxide has a significant effect on lipid accumulation in D. tertiolecta, which provides valuable insights into chemical inducers to enhance biodiesel production in microalgae to solve the problem of diesel shortage.

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Section: Resultssupporting

confidence: 89%

Section: ■ Results and Discussionmentioning

confidence: 99%

Effects of Piperonyl Butoxide on the Accumulation of Lipid and the Transcript Levels of DtMFPα in Dunaliella tertiolecta

Dai

Song

Chen

et al. 2022

J. Agric. Food Chem.

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“…Under normal conditions, cyanobacteria are generally cultured in vitro in a BG-11 medium at 30 °C under agitation and illumination of 50 µmol photons m −2 s −1 [12], the culture being the main form of biomass obtainment [7]. The production of oils from microalgae and cyanobacteria biomass is related to the cultivation conditions to which they are subjected and can stimulate or suppress lipid productivity [13,14]. The optimization of components of crop media, such as CO 2 , H 2 O, N 2 , and factors such as light intensity, pH, and temperature influence the growth of biomass and the lipid content [7].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Light Intensity and NaNO3 Concentration in Amazon Cyanobacteria Cultivation to Produce Biodiesel

et al. 2019

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The objective of this study, for the first time, was to optimize Amazonian cyanobacterial culture conditions for improving cell productivity and lipid content, by analyzing the effect of light intensity and nitrogen concentration, for empirically evaluating biodiesel quality parameters. The strains Synechocystis sp. CACIAM05, Microcystis aeruginosa CACIAM08, Pantanalinema rosaneae CACIAM18, and Limnothrix sp. CACIAM25, were previously identified by morphological and molecular analysis (16S rRNA) and were selected based on their production of chlorophyll a and dry cell weight. Then, factorial planning (22) with central points was applied, with light intensity and NaNO3 concentration as independent variables. As response variables, cell productivity and lipid content were determined. Statistical analysis indicated that for all strains, the independent variables were statistically significant for cell productivity. Analysis of the fatty acid composition demonstrated diversity in the composition of the fatty acid profile from the experimental planning assays of each strain. The Biodiesel Analyzer software predicted the biodiesel quality parameters. CACIAM05 and CACIAM25 obtained better parameters with low levels of light intensity and NaNO3 concentration, whereas CACIAM08 and CACIAM18 obtained better parameters with low NaNO3 concentrations and high luminous intensity.

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“…The rate of cell growth, ranging from 0.0094 day −1 to 0.573 day −1 (Table 1), and the production of quality biocompounds are important variables to determine the viability (cost reduction and efficiency) of bioprospecting microalgae (Dao et al, 2018). The balance between these factors (biomass production and intracellular synthesis) is crucial to achieve maximum productivity and the adequate production of metabolites, leading to studies being performed to improve crops to meet this demand and to make the biotechnological use of microalgae possible (Tsigie et al, 2012; Li et al, 2013; Sforza, Barbera & Bertucco, 2015; He, Yang & Hu, 2015).…”

Section: Introductionmentioning

confidence: 99%

Bioprospection of biocompounds and dietary supplements of microalgae with immunostimulating activity: a comprehensive review

Júnior

Faustino

Cunha

2019

PeerJ

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The objective of this review is to analyze the role of microalgal bioprospecting and the application of microalgae as food supplements and immunostimulants in global and regional aquaculture, highlighting the Brazilian Amazon. This study evaluates the primary advantages of the application of the bioactive compounds of these microorganisms, simultaneously identifying the knowledge gaps that hinder their biotechnological and economic exploitation. The methodology used is comparative and descriptive-analytical, considering the hypothesis of the importance of bioprospecting microalgae, the mechanisms of crop development and its biotechnological and sustainable application. In this context, this review describes the primary applications of microalgae in aquaculture during the last decade (2005–2017). The positive effects of food replacement and/or complementation of microalgae on the diets of organisms, such as their influence on the reproduction rates, growth, and development of fish, mollusks and crustaceans are described and analyzed. In addition, the importance of physiological parameters and their association with the associated gene expression of immune responses in organisms supplemented with microalgae was demonstrated. Complementarily, the existence of technical-scientific gaps in a regional panorama was identified, despite the potential of microalgal cultivation in the Brazilian Amazon. In general, factors preventing the most immediate biotechnological applications in the use of microalgae in the region include the absence of applied research in the area. We conclude that the potential of these microorganisms has been relatively well exploited at the international level but not at the Amazon level. In the latter case, the biotechnological potential still depends on a series of crucial steps that involve the identification of species, the understanding of their functional characteristics and their applicability in the biotechnological area, especially in aquaculture.

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Optimizing light regimes on growth and lipid accumulation in Ankistrodesmus fusiformis H1 for biodiesel production

Cited by 25 publications

References 34 publications

Effects of Piperonyl Butoxide on the Accumulation of Lipid and the Transcript Levels of DtMFPα in Dunaliella tertiolecta

Effects of Piperonyl Butoxide on the Accumulation of Lipid and the Transcript Levels of DtMFPα in Dunaliella tertiolecta

Optimization of Light Intensity and NaNO3 Concentration in Amazon Cyanobacteria Cultivation to Produce Biodiesel

Bioprospection of biocompounds and dietary supplements of microalgae with immunostimulating activity: a comprehensive review

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