Optimizing incubation conditions for the preservation of sperm motility in processed semen samples

Petrella, Carolina; Hsieh, Jennifer; Thrift, K.; Jarow, Jonathan P.; Zacur, Howard A.; Zhao, Yulian

doi:10.1016/j.fertnstert.2005.01.138

Cited by 11 publications

(10 citation statements)

References 11 publications

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“…Until now, several strategies have been developed to improve sperm motility and to increase the motile sperm recovery rate after freezing. Several reports have described improvement in the percentage of motile testicular spermatozoa after a few days of culture at 37 C, or room temperature in cases of obstructive azoospermia (25,26). This evidence is helpful when considering delayed freezing, and when immediate access to an andrology laboratory is not available.…”

Section: Discussionmentioning

confidence: 92%

Emergency testicular sperm extraction after scrotal trauma in a patient with a history of contralateral orchiopexy for cryptorchidism: case report and review of the literature

Gadda

Spinelli

Cozzi

et al. 2012

Fertility and Sterility

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Section: Discussionmentioning

confidence: 92%

Emergency testicular sperm extraction after scrotal trauma in a patient with a history of contralateral orchiopexy for cryptorchidism: case report and review of the literature

Gadda

Spinelli

Cozzi

et al. 2012

Fertility and Sterility

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“…() also found that the concentration of normal motile spermatozoon had an effect on fertilisation and pregnancy rates. Sperm motility characteristics and resistance to the time of incubation have been observed (Zollner et al ., ; Petrella et al ., ). These authors processed semen samples and determined a cut of value of sperm motility to select the suitable reproductive technology (IVF or ICSI).…”

Section: Discussionmentioning

confidence: 97%

Sperm selection based on motility in polyvinylpyrrolidone is associated with successful pregnancy and embryo development

et al. 2012

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The aim of this study was to investigate whether spermatozoon motility in polyvinylpyrrolidone (PVP) is associated with better embryo development and pregnancy rates in ICSI cycles. A total of 123 primary ICSI treatment cycles were included in this study. Semen samples were tested for motility before ICSI procedure in PVP. Within 3 min, the presence or absence of motility was recorded. Sperm functions were examined by the aniline blue (AB) chromatin condensation test and the hypoosmotic swelling test, and the chromatin stability was evaluated by inducing its decondensation with sodium dodecyl sulphate and ethylenediaminetetraacetic acid (EDTA). Fertilisation and embryo scoring were evaluated. Fifty (64%) of 78 women conceived in the PVP (+) group; and 12 (26%) of 45 women conceived in the PVP (-) group; the pregnancy rate was significantly higher in the PVP (+) group (P = 0.003). Semen parameters were observed to be similar in both groups. The mean number of total embryos obtained in ICSI procedure and transferred grade 1 embryos were significantly higher in PVP (+) group (P = 0.01 and P = 0.003 respectively). The presence of sperm motility in PVP is associated with increased pregnancy rate, higher percentage of good quality embryos, sperm chromatin condensation and decondensation.

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“…There are many studies exploring the effects of sperm preparation techniques and the temperature of the environment in which sperms are preserved until insemination on the sperm parameters. However, the number of clinical studies investigating the effect of temperature on the success of IUI is limited ( 7 , 8 , 9 , 10 ) . It has been shown in a study by Petrella et al that sperms prepared with the gradient method and stored at room temperature retain their motility and vitality for a longer period than sperms stored at 4 °C and 37 °C ( 9 ) .…”

Section: Introductionmentioning

confidence: 99%

The effect of preserving prepared sperm samples at room temperature or at 37 °C before intrauterine insemination (IUI) on clinical pregnancy rate

Çok

Aytaç

Şimşek

et al. 2015

tjod

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Objective:The comparison of the effect of preserving prepared sperm samples at room temperature or at 37 °C before intrauterine insemination (IUI) on clinical pregnancy rate.Materials and Methods:Retrospective clinical research. University hospital, infertility clinic. Patients with one or two follicles, between the ages of 20 and 40, whose infertility period was less than 6 years and the injected total motile sperm count was more than 10 million. Preserving sperm samples prepared for IUI at 37 ºC or at room temperature before IUI.The clinical pregnancy rate of IUI cycles between 1st of January 2004 and 1st of December 2011 in which prepared sperm samples were preserved at 37 ºC and the clinical pregnancy rate of IUI cycles between 1st of December 2011 and 31st of May 2014 in which prepared sperm samples preserved at room temperature.Results:Clinical pregnancy rates were similar in IUI cycles in which prepared sperm samples were preserved at 37 ºC and at room temperature (9.3% vs. 8.9%). Clinical pregnancy rates in IUI cycles with 2 follicles were higher than IUI cycles with 1 follicle (10.8% vs. 7.6%) (p=0.002). Further statistical analysis after splitting data according to the number of the follicles revealed that there was no statistical difference between clinical pregnancy rates after IUI cycles in which prepared sperm samples were preserved at 37 ºC or at room temperature in both one follicle (7.6% vs. 7.6%), and two follicle cycles (11.5% vs. 10.1%).Conclusions:Preserving prepared sperm samples at room temperature had no negative effect on clinical pregnancy rates when compared with reserving prepared sperm samples at 37 ºC during IUI cycles.

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Optimizing incubation conditions for the preservation of sperm motility in processed semen samples

Cited by 11 publications

References 11 publications

Emergency testicular sperm extraction after scrotal trauma in a patient with a history of contralateral orchiopexy for cryptorchidism: case report and review of the literature

Emergency testicular sperm extraction after scrotal trauma in a patient with a history of contralateral orchiopexy for cryptorchidism: case report and review of the literature

Sperm selection based on motility in polyvinylpyrrolidone is associated with successful pregnancy and embryo development

The effect of preserving prepared sperm samples at room temperature or at 37 °C before intrauterine insemination (IUI) on clinical pregnancy rate

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