Sperm selection based on motility in polyvinylpyrrolidone is associated with successful pregnancy and embryo development

Abstract

The aim of this study was to investigate whether spermatozoon motility in polyvinylpyrrolidone (PVP) is associated with better embryo development and pregnancy rates in ICSI cycles. A total of 123 primary ICSI treatment cycles were included in this study. Semen samples were tested for motility before ICSI procedure in PVP. Within 3 min, the presence or absence of motility was recorded. Sperm functions were examined by the aniline blue (AB) chromatin condensation test and the hypoosmotic swelling test, and the chromatin stability was evaluated by inducing its decondensation with sodium dodecyl sulphate and ethylenediaminetetraacetic acid (EDTA). Fertilisation and embryo scoring were evaluated. Fifty (64%) of 78 women conceived in the PVP (+) group; and 12 (26%) of 45 women conceived in the PVP (-) group; the pregnancy rate was significantly higher in the PVP (+) group (P = 0.003). Semen parameters were observed to be similar in both groups. The mean number of total embryos obtained in ICSI procedure and transferred grade 1 embryos were significantly higher in PVP (+) group (P = 0.01 and P = 0.003 respectively). The presence of sperm motility in PVP is associated with increased pregnancy rate, higher percentage of good quality embryos, sperm chromatin condensation and decondensation.

Selecting motile, non‐apoptotic and induced spermatozoa for capacitation without centrifuging by MACS‐Up method

“…The viscous layer is made of Poly Vinile Pyrolidin and Carboxymethylcellulose with different ratios. Previous studies revealed these compounds have a little destructive effect on sperm structure (Ahmad et al, 2003; Irez et al, 2013). The volumetric mass of this viscous layer is lower than semen and more than the sperm culture medium and is placed between the two layers (1.02 g/ml).…”

Stain-Free Sperm Analysis and Selection for Intracytoplasmic Sperm Injection Complying with WHO Strict Normal Criteria

“…Sperm quality, including both motility and morphology, is a crucial factor in achieving fertilization and successful pregnancy [5]. Studies have shown that a high rate of sperm motility and normal sperm morphology in a given sample positively correlate with fertilization and pregnancy rates during ICSI [6,7]. Sperm selection during ICSI typically involves an evaluation of sperm shape, size and structure as well as motility parameters under low (X20) magnification.…”