Optimizing in vitro large scale production of giant reed (Arundo donax L.) by liquid medium culture

Cavallaro, Valéria; Patanè, Cristina; Cosentino, Salvatore Luciano; Silvestro, Isabella Di; Copani, Venera

doi:10.1016/j.biombioe.2014.07.004

Cited by 27 publications

(16 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several authors have reported the value of liquid media for shoot multiplication (Russowski et al 2006;Pati et al 2011;Cavallaro et al 2014). However, S. alpina shoot tip explants placed in liquid culture, either agitated or stationary, did not derive shoots, and tissue necrosis was observed, probably caused by the continuous contact between the plant tissue and the liquid medium.…”

Section: Shoot Multiplicationmentioning

confidence: 99%

The influence of liquid systems for shoot multiplication, secondary metabolite production and plant regeneration of Scutellaria alpina

Grzegorczyk-Karolak

Rytczak

Bielecki

et al. 2016

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Section: Shoot Multiplicationmentioning

confidence: 99%

The influence of liquid systems for shoot multiplication, secondary metabolite production and plant regeneration of Scutellaria alpina

Grzegorczyk-Karolak

Rytczak

Bielecki

et al. 2016

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

“…Gellan gum is a bacterial polysaccharide produced by Sphingomonas species (Sa-Correia et al, 2002). Gellan gum has been used for plant tissue culture (Cavallaro et al, 2014), human tissue culture (Gantar et al, 2014) and culturing of water and soil bacteria (Stott et al, 2008). However, a comparison between agar and gellan gum has not been previously reported for endophytic bacterial strains.…”

Section: Introductionmentioning

confidence: 99%

Optimization of isolation and cultivation of bacterial endophytes through addition of plant extract to nutrient media

Eevers

Gielen

Sánchez-López

et al. 2015

Microbial Biotechnology

108

View full text Add to dashboard Cite

Many endophytes have beneficial effects on plants and can be exploited in biotechnological applications. Studies hypothesize that only 0.001–1% of all plant-associated bacteria are cultivable. Moreover, even after successful isolations, many endophytic bacteria often show reduced regrowth capacity. This research aimed to optimize isolation processes and culturing these bacteria afterwards. We compared several minimal and complex media in a screening. Beside the media themselves, two gelling agents and adding plant extract to media were investigated to enhance the number and diversity of endophytes as well as the growth capacity when regrown after isolation.In this work, 869 medium delivered the highest numbers of cultivable bacteria, as well as the highest diversity. When comparing gelling agents, no differences were observed in the numbers of bacteria. Adding plant extract to the media lead to a slight increase in diversity. However, when adding plant extract to improve the regrowth capacity, sharp increases of viable bacteria occurred in both rich and minimal media.

show abstract

“…Although comparable, future yields may decline with this relatively high planting density, as previous studies have shown that after the establishment season, lower planting densities prevailed with higher yields than higher planting densities (Angelini et al 2005;Smith et al 2015). A. donax L. has a reportedly long establishment time in ideal conditions, reaching stabilized dry matter yields three years post-planting (Lewandowski et al 2003;Cavallaro et al 2014;Cosentino et al 2014) perhaps based on its perennial nature (Smith et al 2015).…”

Section: Experiments 3 -Field Soil Drench Trialmentioning

confidence: 93%

“…In vitro tissue culture propagation was performed following modified protocols (Cavallaro et al 2011;Cavallaro et al 2014). Buds were sterilized by rinsing with cold tap water, soaking in 70 % (v/v) bioethanol for one minute, further soaking in 20 % Javex® (1 % available sodium hypochlorite) for twenty minutes and were finally rinsed with autoclaved distilled water.…”

Section: In Vitro Tissue Culture Propagationmentioning

confidence: 99%

Effects of growth-promoters on the productivity of Arundo donax L. (NileFiber™) grown as a purpose-grown biofuel feedstock in Nova Scotia

et al. 2017

View full text Add to dashboard Cite

Abstract:Arundo donax L. is a perennial grass species of Mediterranean origin. Its high biomass yield potential in its native climate has contributed to A. donax being an important biofuel feedstock crop. This study investigates the potential of A. donax as a purpose-grown biomass feedstock for advanced biofuel production in Nova Scotia. The effects of nine different growth-promoters (bacterial and fungal species, a seaweed extract, and a lipo-chitooligosaccharide) and two inoculation methods on A. donax biomass productivity were investigated through three experimental trials: one greenhouse and one field trial inoculated by a post-planting application of the supplements around the plant stem ("soil drenching") and one greenhouse trial with inoculation by root soaking just prior to planting (2016). The survival of A. donax over the winter was also evaluated over two winter seasons (2014-15 and 2015-16). Penicillium bilaii illustrated the most consistent positive effects on biomass productivity across experimental trials, and the plants' higher phosphorus concentrations suggest that P. bilaii was helping the plant access soil phosphorus. The root soaking inoculation method resulted in more positive effects in measured growth parameters compared with the soil drench method. Despite a very mild winter in one of the 2 years tested, A. donax did not successfully overwinter in the field in either year. The presence of growthpromoters did not enhance biomass productivity substantially in the field. Moreover, due to its low over-

show abstract

Optimizing in vitro large scale production of giant reed (Arundo donax L.) by liquid medium culture

Cited by 27 publications

References 33 publications

The influence of liquid systems for shoot multiplication, secondary metabolite production and plant regeneration of Scutellaria alpina

The influence of liquid systems for shoot multiplication, secondary metabolite production and plant regeneration of Scutellaria alpina

Optimization of isolation and cultivation of bacterial endophytes through addition of plant extract to nutrient media

Effects of growth-promoters on the productivity of <i></i><i>Arundo donax</i><i></i> L. (NileFiber™) grown as a purpose-grown biofuel feedstock in Nova Scotia

Contact Info

Product

Resources

About