Optimization of isolation and cultivation of bacterial endophytes through addition of plant extract to nutrient media

Abstract

Many endophytes have beneficial effects on plants and can be exploited in biotechnological applications. Studies hypothesize that only 0.001–1% of all plant-associated bacteria are cultivable. Moreover, even after successful isolations, many endophytic bacteria often show reduced regrowth capacity. This research aimed to optimize isolation processes and culturing these bacteria afterwards. We compared several minimal and complex media in a screening. Beside the media themselves, two gelling agents and adding plant extract to media were investigated to enhance the number and diversity of endophytes as well as the growth capacity when regrown after isolation.In this work, 869 medium delivered the highest numbers of cultivable bacteria, as well as the highest diversity. When comparing gelling agents, no differences were observed in the numbers of bacteria. Adding plant extract to the media lead to a slight increase in diversity. However, when adding plant extract to improve the regrowth capacity, sharp increases of viable bacteria occurred in both rich and minimal media.

Controlling rainfall-induced soil loss from small experimental plots through inoculation of bacteria and cyanobacteria

“…Finally, Chu10 and Bold's basal general media (Andersen, 2005) were selected for cyanobacteria isolation. In addition, Nutrient Agar and Tryptic Soy Agar (TSA) (Eevers et al, 2015) general media were selected for bacteria isolation. The soil bacteria were cultured and isolated according to standard protocols 6 described by Benson (2002).…”

Comparison of specific endophytic bacterial communities in different developmental stages of Passiflora incarnata using culture‐dependent and culture‐independent analysis

“…From the resulting suspension, a series of 10‐fold dilutions down to 10 −4 were prepared. Aliquots (100 µl) of each dilution were spread in triplicates on M9 minimal medium, Gause’s synthetic agar (Zhao, Xu, & Jiang, ), Chitin medium (Lingappa & Lockwood, ), Tap Water Yeast Extract agar (El‐Shatoury, ), Humic acid‐vitamin (HV) agar (Hayakawa & Nonomura, ), Glycerol–asparagine agar (Pridham & Lyons, ) and 869 medium (Eevers et al, ). All media were supplemented with benomyl (50 µg/ml) and cycloheximide (50 µg/ml).…”

Bakteri Endofit dari Tumbuhan Paku-pakuan sebagai Agens Hayati Rhizoctonia solani dan Pemacu Pertumbuhan Tanaman Padi

“…Kelimpahan bakteri endofit dari ketiga jenis tumbuhan paku yang diisolasi memiliki populasi yang berbeda. Perbedaan populasi bakteri yang diperoleh dipengaruhi oleh bagian tanaman sebagai sumber endofit dan (Eevers et al 2015;Hallmann et al 1997). Salvinia molesta merupakan jenis paku air yang memiliki kelimpahan bakteri endofit lebih banyak dibandingkan dengan bakteri yang diisolasi dari Pteris ensiformis (paku terestrial) dan Drymoglossum pilosilloides (paku epifit).…”