Optimizing high‐performance liquid chromatography method for quantification of glucosamine using 6‐aminoquinolyl‐<i>N</i>‐hydroxysuccinimidyl carbamate derivatization in rat plasma: application to a pharmacokinetic study

Li, Linyu; Chen, Xiang; Chen, Lijuan; Wang, Bi‐Qin; Peng, Cheng; He, Chunmei; Tang, Minghai; Zhang, Fan; Hu, Jia; Li, Rui; Zhao, Xia; Wei, Yuquan

doi:10.1002/bmc.1056

Cited by 24 publications

(20 citation statements)

References 14 publications

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“…The GE content was determined using an adiabatic calorimetric bomb (C7000; IKA, Staufen, Germany). Chitin was analyzed as D-Glucosamine [28] using a modification of the method described by Madrid et al [29] for AA.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the suitability of a partially defatted black soldier fly (Hermetia illucens L.) larvae meal as ingredient for rainbow trout (Oncorhynchus mykiss Walbaum) diets

Renna

Schiavone

Gai

et al. 2017

J Animal Sci Biotechnol

295

245

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BackgroundTwo trials were performed to evaluate a partially defatted Hermetia illucens (HI) larvae meal as potential feed ingredient in rainbow trout (Oncorhynchus mykiss Walbaum) diets. In the first trial, 360 trout (178.9 ± 9.8 g of mean initial body weight) were randomly divided into three experimental groups (4 tanks/treatment, 30 fish/tank). The fish were fed for 78 days with isonitrogenous, isolipidic and isoenergetic diets containing increasing levels of HI, on as fed basis: 0% (HI0, control diet), 25% (HI25) and 50% (HI50) of fish meal substitution, corresponding to dietary inclusion levels of 0, 20% and 40%. In the second trial, 36 trout (4 tanks/treatment, 3 fish/tank) were used to evaluate the in vivo apparent digestibility coefficients (ADC) of the same diets used in the first trial.ResultsSurvival, growth performance, condition factor, somatic indexes, and dorsal fillet physical quality parameters were not affected by diet. The highest dietary inclusion of HI larvae meal increased dry matter and ether extract contents of trout dorsal fillet. The use of HI larvae meal induced a decrease of valuable polyunsaturated fatty acids (PUFA) even if differences were only reported at the highest level of HI inclusion. The insect meal worsened the lipids health indexes of the same muscle. Dietary inclusion of insect meal did not alter the villus height of the fish. No differences were found among treatments in relation to ADC of ether extract and gross energy, while ADC of dry matter and crude protein were higher in HI25 if compared to HI50.ConclusionsThe obtained results showed that a partially defatted HI larvae meal can be used as feed ingredient in trout diets up to 40% of inclusion level without impacting survival, growth performance, condition factor, somatic indexes, dorsal fillet physical quality parameters, and intestinal morphology of the fish. However, further investigations on specific feeding strategies and diet formulations are needed to limit the observed negative effects of the insect meal on the FA composition of dorsal muscle.

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Section: Methodsmentioning

confidence: 99%

Evaluation of the suitability of a partially defatted black soldier fly (Hermetia illucens L.) larvae meal as ingredient for rainbow trout (Oncorhynchus mykiss Walbaum) diets

Renna

Schiavone

Gai

et al. 2017

J Animal Sci Biotechnol

295

245

View full text Add to dashboard Cite

show abstract

“…Plasma was derivatized60 with AccQ-Fluor Reagent Kit (Waters, Milford, MA, USA) according to the manufacturer’s instructions. HPLC was performed on Nexera sytem equipped with degasser DGU-20/A5, auto-sampler SIL-30AC, column oven CTO-20AC and fluorescence detector RF-20AXS (all obtained from Shimadzu, Kyoto, Japan).…”

Section: Methodsmentioning

confidence: 99%

D-Glucosamine supplementation extends life span of nematodes and of ageing mice

Priebs²,

et al. 2014

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D-Glucosamine (GlcN) is a freely available and commonly used dietary supplement potentially promoting cartilage health in humans, which also acts as an inhibitor of glycolysis. Here we show that GlcN, independent of the hexosamine pathway, extends Caenorhabditis elegans life span by impairing glucose metabolism that activates AMP-activated protein kinase (AMPK/AAK-2) and increases mitochondrial biogenesis. Consistent with the concept of mitohormesis, GlcN promotes increased formation of mitochondrial reactive oxygen species (ROS) culminating in increased expression of the nematodal amino acid-transporter 1 (aat-1) gene. Ameliorating mitochondrial ROS formation or impairment of aat-1-expression abolishes GlcN-mediated life span extension in an NRF2/SKN-1-dependent fashion. Unlike other calorie restriction mimetics, such as 2-deoxyglucose, GlcN extends life span of ageing C57BL/6 mice, which show an induction of mitochondrial biogenesis, lowered blood glucose levels, enhanced expression of several murine amino-acid transporters, as well as increased amino-acid catabolism. Taken together, we provide evidence that GlcN extends life span in evolutionary distinct species by mimicking a low-carbohydrate diet.

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“…MAPK genes respond to stress stimuli such as oxidative stress, which activate enzymes to oxidize AB‐FUBINACA. Studies have been shown that MAPK cascade and downstream transcription factors represent key regulatory components of reactive oxygen species signaling [Skopelitis et al, 2006; Wang et al, 2008; Schmidt et al, 2013]. …”

Section: Discussionmentioning

confidence: 99%

Detection and Characterization of the Effect of AB‐FUBINACA and Its Metabolites in a Rat Model

Chen

Dip

Ahmed

et al. 2015

J of Cellular Biochemistry

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Synthetic cannabinoids were originally developed by academic and pharmaceutical laboratories with the hope of providing therapeutic relief from the pain of inflammatory and degenerative diseases. However, recreational drug enthusiasts have flushed the market with new strains of these potent drugs that evade detection yet endanger public health and safety. Although many of these drug derivatives were published in the medical literature, others were merely patented without further characterization. AB‐FUBINACA is an example of one of the new indazole‐carboxamide synthetic cannabinoids introduced in the past year. Even though AB‐FUBINACA has become increasingly prominent in forensic drug and toxicology specimens analyses, little is known about the pharmacology of this substance. To study its metabolic fate, we utilized Wistar rats to study the oxidative products of AB‐FUBINACA in urine and its effect on gene expressions in liver and heart. Rats were injected with 5 mg/kg of AB‐FUBINACA each day for 5 days. Urine samples were collected every day at the same time. On day 5 after treatment, we collected the organs such as liver and heart. The urine samples were analyzed by mass spectrometry, which revealed several putative metabolites and positioning of the hydroxyl addition on the molecule. We used quantitative PCR gene expression array to analyze the hepatotoxicity and cardiotoxicity on these rats and confirmed by real‐time quantitative RT‐PCR. We identified three genes significantly associated with dysfunction of oxidation and inflammation. Our study reports in vivo metabolites of AB‐FUBINACA in urine and its effect on the gene expressions in liver and heart. J. Cell. Biochem. 117: 1033–1043, 2016. © 2015 The Authors. Journal of Cellular Biochemistry Published by Wiley Periodicals. Inc.

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Optimizing high‐performance liquid chromatography method for quantification of glucosamine using 6‐aminoquinolyl‐N‐hydroxysuccinimidyl carbamate derivatization in rat plasma: application to a pharmacokinetic study

Cited by 24 publications

References 14 publications

Evaluation of the suitability of a partially defatted black soldier fly (Hermetia illucens L.) larvae meal as ingredient for rainbow trout (Oncorhynchus mykiss Walbaum) diets

Evaluation of the suitability of a partially defatted black soldier fly (Hermetia illucens L.) larvae meal as ingredient for rainbow trout (Oncorhynchus mykiss Walbaum) diets

D-Glucosamine supplementation extends life span of nematodes and of ageing mice

Detection and Characterization of the Effect of AB‐FUBINACA and Its Metabolites in a Rat Model

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