Optimizing cyanobacteria growth conditions in a sealed environment to enable chemical inhibition tests with volatile chemicals

Johnson, Tylor J.; Zahler, Jacob D.; Baldwin, Emily L.; Zhou, Ruanbao; Gibbons, William R.

doi:10.1016/j.mimet.2016.05.011

Cited by 10 publications

(6 citation statements)

References 36 publications

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“…The higher growth rate of Synechococcus 7002 was obtained under strong light intensity. Johnson et al cultured Anabaena sp. PCC 7120 in the BG‐11 medium with 0.5 g/L NaHCO 3 as a carbon source in some sealed test tubes.…”

Section: Effective Physical Parametersmentioning

confidence: 99%

Cyanobacteria as an eco‐friendly resource for biofuel production: A critical review

Farrokh

Sheikhpour

Kasaeian³

et al. 2019

Biotechnology Progress

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Cyanobacteria are photosynthetic microorganisms which can be found in various environmental habitats. These photosynthetic bacteria are considered as promising feedstock for the production of the third‐ and the fourth‐generation biofuels. The main subject of this review is highlighting the significant aspects of the biofuel production from cyanobacteria. The most recent investigations about the extraction or separation of the bio‐oil from cyanobacteria are also adduced in the present review. Moreover, the genetic engineering of cyanobacteria for improving biofuel production and the impact of bioinformatics studies on the designing better‐engineered strains are mentioned. The large‐scale biofuel production is challenging, so the economic considerations to provide inexpensive biofuels are also cited. It seems that the future of biofuels is strongly dependent to the following items; understanding the metabolic pathways of the cyanobacterial species, progression in the construction of the engineered cyanobacteria, and inexpensive large‐scale cultivation of them.

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Section: Effective Physical Parametersmentioning

confidence: 99%

Cyanobacteria as an eco‐friendly resource for biofuel production: A critical review

Farrokh

Sheikhpour

Kasaeian³

et al. 2019

Biotechnology Progress

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“…As a carbon source, NaHCO 3 (sodium bicarbonate) supplementation is substantially used for cyanobacterial cell growth instead of CO 2 gas bubbling [ 30 ], resulting in a pool of HCO 3 − , which is subsequently supplied with more CO 2 in the carboxysome [ 17 ]. We found a higher growth rate and shorter doubling time of both OX rbcL and OX rbcLXS under sodium bicarbonate supplementation when compared to WTc, whereas there were no significant changes under the normal BG 11 condition ( Figure 3 A,B).…”

Section: Resultsmentioning

confidence: 99%

Increased Biomass and Polyhydroxybutyrate Production by Synechocystis sp. PCC 6803 Overexpressing RuBisCO Genes

Tharasirivat

Jantaro

2023

IJMS

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The overexpression of the RuBisCO (rbc) gene has recently become an achievable strategy for increasing cyanobacterial biomass and overcoming the biocompound production restriction. We successfully constructed two rbc-overexpressing Synechocystis sp. PCC 6803 strains (OX), including a strain overexpressing a large subunit of RuBisCO (OXrbcL) and another strain overexpressing all large, chaperone, and small subunits of RuBisCO (OXrbcLXS), resulting in higher and faster growth than wild type under sodium bicarbonate supplementation. This increased biomass of OX strains significantly contributed to the higher polyhydroxybutyrate (PHB) production induced by nutrient-deprived conditions, in particular nitrogen (N) and phosphorus (P). As a result of higher PHB contents in OX strains occurring at days 7 and 9 of nutrient deprivation, this enhancement was apparently made possible by cells preferentially maintaining their internal lipids while accumulating less glycogen. The OXrbcLXS strain, with the highest level of PHB at about 39 %w/dry cell weight (DCW) during 7 days of BG11-NP treatment, contained a lower glycogen level (31.9 %w/DCW) than wild type control (40 %w/DCW). In contrast, the wild type control strain exposed to N- and NP-stresses tended to retain lipid levels and store more glycogen than PHB. In this model, we, for the first time, implemented a RuBisCO-overexpressing cyanobacterial factory for overproducing PHB, destined for biofuel and biomaterial biotechnology.

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“…There CO 2 is derived from HCO 3 − via carbonic anhydrase, which maintains a steady state flux to ribulose-1.5-bisphosphate carboxylase oxygenase for photosynthesis [85]. It has been determined by our research group that 0.5 g/L NaHCO 3 is the optimal concentration for cultivating filamentous cyanobacteria in a sealed environment [37]. This method was adapted from previous studies that used a sealed environment for chemical inhibition tests with algae [33,52].…”

Section: Methodsmentioning

confidence: 99%

Increasing the tolerance of filamentous cyanobacteria to next-generation biofuels via directed evolution

et al. 2016

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Optimizing cyanobacteria growth conditions in a sealed environment to enable chemical inhibition tests with volatile chemicals

Cited by 10 publications

References 36 publications

Cyanobacteria as an eco‐friendly resource for biofuel production: A critical review

Cyanobacteria as an eco‐friendly resource for biofuel production: A critical review

Increased Biomass and Polyhydroxybutyrate Production by Synechocystis sp. PCC 6803 Overexpressing RuBisCO Genes

Increasing the tolerance of filamentous cyanobacteria to next-generation biofuels via directed evolution

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