Increasing the tolerance of filamentous cyanobacteria to next-generation biofuels via directed evolution

Johnson, Tylor J.; Halfmann, Charles; Zahler, Jacob D.; Zhou, Ruanbao; Gibbons, William R.

doi:10.1016/j.algal.2016.06.023

Cited by 16 publications

(6 citation statements)

References 91 publications

(99 reference statements)

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“…PCC 7120 strain with a 220% increase in tolerance to farnesene compared to the wildtype, an Anabaena variabilis sp. ATCC 29413 strain with a 60% increase in tolerance to linalool compared to the wildtype, and a Nostoc punctiforme ATCC 29133 strain with a 20% increase in tolerance to linalool compared to the wildtype . Other potential targets for cyanobacterial directed evolution studies include: carotenoid biosynthesis, polyhydoxyalkanoate biosynthesis, and modification of enzymes to maintain their catalytic function under variable production conditions …”

Section: Mutagenesis Improvements To Cyanobacteria For Industrial Appmentioning

confidence: 99%

“…However, a separate mutagenesis or genetic engineering step may be necessary after increased tolerance is achieved in order to also increase production. For example, our research group used directed evolution to develop several filamentous cyanobacterial mutants with an increased tolerance to linalool or farnesene . Now these strains can be genetically engineered to produce these biofuels.…”

Section: Mutagenesis Improvements To Cyanobacteria For Industrial Appmentioning

confidence: 99%

“…For example, our research group used directed evolution to develop several filamentous cyanobacterial mutants with an increased tolerance to linalool or farnesene. 164 Now these strains can be genetically engineered to produce these biofuels. If the cyanobacterial strain was genetically engineered first, non-site directed mutagenesis techniques could interrupt the genes responsible for biofuel production.…”

Section: Strategies To Improve Tolerance To Biofuels or Chemicalsmentioning

confidence: 99%

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Molecular genetic improvements of cyanobacteria to enhance the industrial potential of the microbe: A review

Johnson

Gibbons

et al. 2016

Biotechnology Progress

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The rapid increase in worldwide population coupled with the increasing demand for fossil fuels has led to an increased urgency to develop sustainable sources of energy and chemicals from renewable resources. Using microorganisms to produce high-value chemicals and next-generation biofuels is one sustainable option and is the focus of much current research. Cyanobacteria are ideal platform organisms for chemical and biofuel production because they can be genetically engineered to produce a broad range of products directly from CO , H O, and sunlight, and require minimal nutrient inputs. The purpose of this review is to provide an overview on advances that have been or could be made to improve strains of cyanobacteria for industrial purposes. First, the benefits of using cyanobacteria as a platform for chemical and biofuel production are discussed. Next, an overview of cyanobacterial strain improvements by genetic engineering is provided. Finally, mutagenesis techniques to improve the industrial potential of cyanobacteria are described. Along with providing an overview on various areas of research that are currently being investigated to improve the industrial potential of cyanobacteria, this review aims to elucidate potential targets for future research involving cyanobacteria as an industrial microorganism. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1357-1371, 2016.

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Section: Mutagenesis Improvements To Cyanobacteria For Industrial Appmentioning

confidence: 99%

Section: Mutagenesis Improvements To Cyanobacteria For Industrial Appmentioning

confidence: 99%

Section: Strategies To Improve Tolerance To Biofuels or Chemicalsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular genetic improvements of cyanobacteria to enhance the industrial potential of the microbe: A review

Johnson

Gibbons

et al. 2016

Biotechnology Progress

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“…Such trait combinations are normally mutually exclusive. Approaches include genetic engineering (Zeng et al, 2011), directed evolution via successive rounds of mutagenesis and selection (Johnson et al, 2016;Lewin et al, 2016), and selection on the existing levels of genetic variation in a population (Mooij et al, 2013;Shurin et al, 2016).…”

Section: Renewable Biofuels: When Evolution Fills Our Tanksmentioning

confidence: 99%

On biological evolution and environmental solutions

Matthews

Jokela

Narwani

et al. 2020

Science of The Total Environment

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Drawing insights from multiple disciplines is essential for finding integrative solutions that are required to tackle complex environmental problems. Human activities are causing unprecedented influence on global ecosystems, culminating in the loss of species and fundamental changes in the selective environments of organisms across the tree of life. Our collective understanding about biological evolution can help identify and mitigate many of the environmental problems in the Anthropocene. To this end, we propose a stronger integration of environmental sciences with evolutionary biology.

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“…PCC 7120 strain that, when compared to wild-type, exhibited a 220% increase in tolerance to farnesene (C 15 H 24 ). We also developed an Nostoc punctiforme ATCC 29133 strain with a 20% increase in tolerance to linalool [27]. Next we will genetically engineer these strains to produce the chemicals they have increased tolerance to.…”

Section: Introductionmentioning

confidence: 99%

Outlook on the Potential of Cyanobacteria to Photosynthetically Produce High- Value Chemicals and Biofuels at an Industrial Scale

Johnson¹,

Zhou²,

Johnson³

et al. 2016

Bioenergetics

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As the need to develop sustainable and renewable sources of chemicals and energy is increasing, cyanobacteria have emerged as an attractive industrial microorganism. Using energy from sunlight, H 2 O, and CO 2 , cyanobacteria can either produce naturally or be genetically engineered to produce high-value chemicals and next-generation biofuels. The list of chemicals that cyanobacteria have produced is extensive and constantly growing, however the titers of the chemicals produced by cyanobacteria are generally low. Thus, before an economically feasible large-scale chemical production process can be achieved, cyanobacterial production titers must be increased. Also, costs associated with cultivating cyanobacteria at an industrial scale must be decreased. In this communication, our research group's progress towards enhancing the industrial potential of cyanobacteria is summarized and potential future targets for research are discussed. Cyanobacteria as industrial microorganisms holds a great deal of potential towards developing high-value chemicals and next-generation biofuels.

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Increasing the tolerance of filamentous cyanobacteria to next-generation biofuels via directed evolution

Cited by 16 publications

References 91 publications

Molecular genetic improvements of cyanobacteria to enhance the industrial potential of the microbe: A review

Molecular genetic improvements of cyanobacteria to enhance the industrial potential of the microbe: A review

On biological evolution and environmental solutions

Outlook on the Potential of Cyanobacteria to Photosynthetically Produce High- Value Chemicals and Biofuels at an Industrial Scale

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