Optimizing Crop Production with Bacterial Inputs: Insights into Chemical Dialogue between Sphingomonas sediminicola and Pisum sativum

Abstract

The use of biological inputs is an interesting approach to optimize crop production and reduce the use of chemical inputs. Understanding the chemical communication between bacteria and plants is critical to optimizing this approach. Recently, we have shown that Sphingomonas (S.) sediminicola can improve both nitrogen supply and yield in pea. Here, we used biochemical methods and untargeted metabolomics to investigate the chemical dialog between S. sediminicola and pea. We also evaluated the metabolic capacities of S. sediminicola by metabolic profiling. Our results showed that peas release a wide range of hexoses, organic acids, and amino acids during their development, which can generally recruit and select fast-growing organisms. In the presence of S. sediminicola, a more specific pattern of these molecules took place, gradually adapting to the metabolic capabilities of the bacterium, especially for pentoses and flavonoids. In turn, S. sediminicola is able to produce several compounds involved in cell differentiation, biofilm formation, and quorum sensing to shape its environment, as well as several molecules that stimulate pea growth and plant defense mechanisms.

Decrease in beneficial bacteria and increase in harmful bacteria in Gastrodia seedlings and their surrounding soil are mainly responsible for degradation of Gastrodia asexual propagation

“…In this study, it decreased with the increase of reproductive generations, and it was the dominant bacterium in the first generation of healthy GeB, which was verified to be a probiotic for GeB seedlings. Sphingomonas ( Li et al., 2019 ) can symbiosis with plants and can play a role in antagonizing plant pathogenic fungi and promoting plant growth, which increases with the increase of reproductive generations ( Mazoyon et al., 2023 ). However, further in-depth research is required to determine whether this is probiotic.…”

Sphingomonas sediminicola Dae20 Is a Highly Promising Beneficial Bacteria for Crop Biostimulation Due to Its Positive Effects on Plant Growth and Development

“…As previously reported, S. sediminicola was found to be highly abundant in agricultural soils after pea culture [ 41 ]. This occurrence was found to be non-random, as the pea plant adjusts the composition of its root exudates to specifically attract this bacterium [ 34 ]. Once recruited, this bacterium promotes pea growth, induces the formation of root nodules, and fixes atmospheric nitrogen [ 20 ].…”

“…With a demonstrated ability to increase legume productivity regardless of soil nitrogen content, this bacterium offers a promising alternative to conventional rhizobial inoculants, especially in soils that are not nitrogen poor [ 20 ]. The significant abundance of S. sediminicola Dae20 in agricultural soils following pea cultivation is not a coincidence, but rather a strategic preference of the crop [ 34 , 41 ]. This strategic choice highlights the potential role of S. sediminicola Dae20 in shaping soil microbial communities, thus influencing nutrient cycling and availability.…”

“…In addition, an increase in the fresh weight of both shoot and root components was observed when VOCs were applied (Figure 2c,d). As many bacteria such as S. sediminicola Dae20 can produce both soluble [34] and volatile organic compounds (VOCs) [35], we wanted to know the contribution of VOCs to the plant phenotypic changes induced by S. sediminicola Dae20. In the presence of only these VOCs, there was no detectable change in primary root growth or LR formation (Figure 2a,b) compared to non-inoculated plants.…”

Agricultural Bioinputs Obtained by Solid-State Fermentation: From Production in Biorefineries to Sustainable Agriculture

“…The methods of application range from seed coating application, root application, and foliar application, as well as incorporation directly into the soil to improve nutrient availability. Examples of microorganisms that promote plant growth include bacteria of the genera Rhizobium, Azospirillum, Bacillus, Streptomyces, Gluconoacetobacter, and Pseudomonas and filamentous mycorrhizal fungi such as Claroideoglomus, Glomus, and Rhizophagus, which are used in the production of bioinoculants [12][13][14]. Recently, there has also been an increase in studies on yeasts such as those of the genera Candida spp., Rhodotorula spp., Cryptococcus spp., and Saccharomyces sp., which have been reported to promote plant growth [15].…”