Optimizing Agrobacterium-mediated transformation of grapevine

Li, Z. T.; Dhekney, Sadanand A.; Dutt, Manjul; Aman, M. Van; Tattersall, J.; Kelley, Karen; Gray, D. J.

doi:10.1079/ivp2006770

Cited by 79 publications

(62 citation statements)

References 35 publications

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“…In grapevines, this technology has been recently applied to analyze the function of several genes such as VviAdh [75], VviPIP2 [76], VviCCD1 [77], and VviWRKY33 [78]. Likewise, this method has been proved to be very useful for transgenic complementation [79][80][81], technical studies [82][83][84][85][86][87], promoter analysis [88][89][90][91], subcellular localization analysis [92], and protein production [69,93] in grapevine. Nonetheless, the genetic background and plant growth conditions are key factors in performing successful Agrobacterium-mediated transformation.…”

Section: Indirect Gene Transfer Methods: Agrobacterium-mediated Transmentioning

confidence: 99%

“…For instance, the compatibility of A. tumefaciens strains with the plant species represents an important variable to be considered in this kind of assay. Thus, the strains most efficiently used for gene transfer into grapevine are probably C58C1 (pCH32) [69,78,86] and EHA105 [75,77,82,83,85,88,90], which contain extra copies of vir genes that make them hypervirulent [61].…”

Section: Indirect Gene Transfer Methods: Agrobacterium-mediated Transmentioning

confidence: 99%

“…A number of grapevine cultivars have been stably transformed using Agrobacterium-mediated procedures and most progress has been achieved using embryogenic cell masses [82,83,85,93]. Sonication-assisted Agrobacterium-mediated transformation (SAAT) was first reported by Trick and Finer [98], who used this technique for the production of transgenic soybean (Glycine max) and Ohio buckeye (Aesculus glabra) plants from SAAT-treated embryogenic cell suspensions.…”

Section: Sonication-assisted Agrobacterium-mediated Transformation (Smentioning

confidence: 99%

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Development and Use of Biotechnology Tools for Grape Functional Analysis

Chialva¹,

Eichler²,

Muñoz³

et al. 2016

Grape and Wine Biotechnology

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The aim of this chapter is to provide a description of the latest scientific advances in the field of gene functional analysis in grapevine. It provides general information about the studies conducted during the past decade to understand the natural variation of this plant and how this information has been exploited for the understanding of traits of interest. Likewise, it is exposed how the use of biotechnology tools have helped to characterize the mechanisms of gene expression and its regulation, as well as the subcellular localization of proteins and their interactions with other molecules. Finally, an approximation to the new technologies of gene editing and their potential application in the functional study of grapevine has been carried out.

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Section: Indirect Gene Transfer Methods: Agrobacterium-mediated Transmentioning

confidence: 99%

Section: Indirect Gene Transfer Methods: Agrobacterium-mediated Transmentioning

confidence: 99%

Section: Sonication-assisted Agrobacterium-mediated Transformation (Smentioning

confidence: 99%

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Development and Use of Biotechnology Tools for Grape Functional Analysis

Chialva¹,

Eichler²,

Muñoz³

et al. 2016

Grape and Wine Biotechnology

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“…The positive result of the transformation was shown by green luminescens from GFP protein, which was the result from transient expression of the gfp reporter gene expression in transformed embryogenic callus and somatic embryos, gfp gene is a gene that encodes a protein green fluorescence, when the gene is expressed it will produce a glowing green in parts of transformed plants or culture. GFP expression had been successfully used to test the success of transformation, including the embryogenic callus of sweet orange (Citrus sinensis (L.) Osbeck) [32] and somatic embryos of grapes (Vitis vinifera L. cv.Thompson Seedless) [33].…”

Section: Observations Of Gfp Reporter Gene Expression In Embryogenic mentioning

confidence: 99%

“…years [33]. GFP expression was stable until the plant survived and regenerated and it expressed in various tissues and organs of the plant (buds, leaves, roots, petal, stamen), even in flowers, fruits and seeds [16].…”

Section: Observations Of Gfp Reporter Gene Expression In Embryogenic mentioning

confidence: 99%

Agrobacterium tumefaciens-mediated Transformation of Embryogenic Callus and Somatic Embryos of the Banana cv “Ambon Lumut” (Musa acuminata)

Kusumastuti¹,

Esyantia²,

Dwivany³

2017

KLS

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Banana is one of the major fruit crops, though its conventional breeding has limitations, such as sterility and high polyploidy levels. Biotechnological approach using genetic transformation crop for improvement offers an alternative solution. In this study a protocol was developed for establishing genetic transformation from embryogenic callus and somatic embryos of the banana cv Ambon Lumut. Embryogenic callus was

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Grapes

Bouquet

Torregrosa

Iocco

et al. 2008

Compendium of Transgenic Crop Plants

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Grapevine ( Vitis vinifera ) is one of the major fruit crops in the world based on hectares cultivated and economic value, essentially due to the production of wine. The premium quality wines are produced from cultivars that enjoy a high level of consumer acceptance and are firmly entrenched in the market place. Transgenic breeding is attractive when compared to conventional breeding, because it offers a means of inserting new characters, such as disease resistance, into the genome of traditional cultivars without changing any of their other characteristics particularly wine typicity and quality. The aim of this review is to provide an outlook on the usefulness of genetic transformation in grapevine, not only to develop genetically modified cultivars for commercial release, but also as a valuable tool to understand the genetics and mechanisms of plant growth, development, and metabolism. Thus, the availability of improved and efficient techniques of transformation is needed to sustain the current development of grapevine genomics. Even if the future development of transgenic cultivars will depend essentially on a general acceptance of GM plant products by the consumers, it will require a complete and reliable assessment of their environmental and health safety, comparing the potential risks to the expected benefits of their use.

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Optimizing Agrobacterium-mediated transformation of grapevine

Cited by 79 publications

References 35 publications

Development and Use of Biotechnology Tools for Grape Functional Analysis

Development and Use of Biotechnology Tools for Grape Functional Analysis

Agrobacterium tumefaciens-mediated Transformation of Embryogenic Callus and Somatic Embryos of the Banana cv “Ambon Lumut” (Musa acuminata)

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