Optimizing Ablative Therapy: Manipulating the Microenvironment

Baust, John M.; Santucci, Kimberly L.; Gage, Andrew A.; Robilotto, Anthony T.

doi:10.1007/978-1-62703-182-0_26

Cited by 3 publications

(6 citation statements)

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“…For example, the −25°C critical isotherm found for PaCa in vitro is much higher than the −40°C critical isotherm reported for prostate cancer. 16,26 –28 The PaCa response was similar to the −20°C isotherm reported for renal carcinoma. 36 For both prostate and renal cancer, decreasing levels of survival have been reported following exposure to −15°C, −20°C, −30°C, and lower; however, it is not until the minimum lethal temperature is attained that complete cell death is achieved.…”

Section: Discussionsupporting

confidence: 79%

“…37 Despite the approximately 50% cell death expressed following exposure to −15°C, these reports have shown that complete destruction of prostate cancer cells is not achieved until temperatures reach −40°C or below in vitro . 26 –28 , 35,37 In regard to PaCa cells, in this study, we observed a moderate impact on the viability at −15°C or warmer, whereas temperatures below −20°C resulted in a more severe decrease in cell viability. Gage et al suggest that while temperatures within the range of −20°C are sufficient to cause significant cellular damage, temperatures below −40°C should be sought to ensure complete tissue destruction during freezing.…”

Section: Discussionmentioning

confidence: 56%

“…Previous reports have indicated that repeated exposure of cancer cells to a thermal insult results in increased cell death. 18–19,25 –27 To this end, we investigated the impact of a double freeze (F/T/F) or a double heat (H/T/H) protocol on PaCa survival. In PANC-1 samples, a double freeze exposure to −15°C resulted in an initial decrease in day 1 viability compared to a single freeze, 65% (±4) versus 97% (±3), respectively.…”

Section: Resultsmentioning

confidence: 99%

“…16 –21 Cryoablation subjects tissues to multiple cellular stresses beyond freezing, including hypoxia, osmotic imbalance, ischemia, and other forms of molecular stress. 16,21,25 –27 One of the main uses of cryotherapy is the treatment of prostate cancer. In 2008, the American Urological Association (AUA) published a best practice statement on cryotherapy for the treatment of prostate cancer.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Pancreatic Cancer Cell Thermal Response to Heat Ablation or Cryoablation

Baumann

Baust

Snyder

et al. 2016

Technol Cancer Res Treat

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One of the most lethal carcinomas is pancreatic cancer. As standard treatment using chemotherapy and radiation has shown limited success, thermal regimens (cryotherapy or heat ablation) are emerging as viable alternatives. Although promising, our understanding of pancreatic cancer response to thermal ablation remains limited. In this study, we investigated the thermal responses of 2 pancreatic cancer cell lines in an effort to identify the minimum lethal temperature needed for complete cell death to provide guidance for in vivo applications. PANC-1 and BxPC-3 were frozen (À10 C to À25 C) or heated (45 C-50 C) in single and repeated exposure regimes. Posttreatment survival and recovery were analyzed using alamarBlue assay over a 7-day interval. Modes of cell death were assessed using fluorescence microscopy (calcein acetoxymethyl ester/propidium iodide) and flow cytometry (YO-PRO-1/propidium iodide). Freezing to À10C resulted in minimal cell death. Exposure to À15 C had a mild impact on PANC-1 survival (93%), whereas BxPC-3 was more severely damaged (33%). Exposure to À20 C caused a significant reduction in viability (PANC-1 ¼ 23%; BxPC-3 ¼ 2%) whereas À25 C yielded complete death. Double freezing exposure was more effective than single exposure. Repeat exposure to À15 C resulted in complete death of BxPC-3, whereas À20 C severely impacted PANC-1 (7%). Heating to 45 C resulted in minimum cell death. Exposure to 48 C yielded a slight increase in cell loss (PANC-1 ¼ 85%; BxPC-3 ¼ 98%). Exposure to 50 C caused a significant decline (PANC-1 ¼ 70%; BxPC-3 ¼ 9%) with continued deterioration to 0%. Double heating to 45 C resulted in similar effects observed in single exposures, whereas repeated 48 C resulted in significant increases in cell death (PANC-1 ¼ 68%; BxPC-3 ¼ 29%). In conclusion, we observed that pancreatic cancer cells were completely destroyed at temperatures <À25 C or >50 C using single thermal exposures. Repeated exposures resulted in increased cell death at less extreme temperatures. Our data suggest that thermal ablation strategies (heat or cryoablation) may represent a viable technique for the treatment of pancreatic cancer.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 56%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of Pancreatic Cancer Cell Thermal Response to Heat Ablation or Cryoablation

Baumann

Baust

Snyder

et al. 2016

Technol Cancer Res Treat

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“…Super-resolution imaging algorithms and similar techniques using the Doppler effect are in progress. One recent, interesting subject in the literature is the development of fusion images from the 3-D US volumes with other imaging modalities such as fusion 3-D US and MRI imaging for prostate biopsy (Baust et al 2013;Hu et al 2017;Natarajan et al 2011;Sierra et al 2015;Xu et al 2008), which could provide physicians with valuable information for better diagnosis and treatment. Many modern commercial US systems have been used with 3-D probes, but they are usually manufactured with closed-source platforms, which is an obstacle to improving 3-D US imaging in academia.…”

Section: Discussionmentioning

confidence: 99%

Freehand 3-D Ultrasound Imaging: A Systematic Review

Mozaffari

Lee

2017

Ultrasound in Medicine & Biology

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Two-dimensional ultrasound (US) imaging has been successfully used in clinical applications as a low-cost, portable and non-invasive image modality for more than three decades. Recent advances in computer science and technology illustrate the promise of the 3-D US modality as a medical imaging technique that is comparable to other prevalent modalities and that overcomes certain drawbacks of 2-D US. This systematic review covers freehand 3-D US imaging between 1970 and 2017, highlighting the current trends in research fields, the research methods, the main limitations, the leading researchers, standard assessment criteria and clinical applications. Freehand 3-D US systems are more prevalent in the academic environment, whereas in clinical applications and industrial research, most studies have focused on 3-D US transducers and improvement of hardware performance. This topic is still an interesting active area for researchers, and there remain many unsolved problems to be addressed.

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