Optimized preparation of daidzein-loaded chitosan microspheres and in vivo evaluation after intramuscular injection in rats

Ge, Yuebin; Chen, Dawei; Xie, Liping; Zhang, Rongqing

doi:10.1016/j.ijpharm.2007.01.046

Cited by 35 publications

(32 citation statements)

References 26 publications

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“…The incubation times for these groups were 1, 3, 6, 12, 24 h respectively, while the last group served as control without mixing with microspheres. The microsphere concentration (10mg/10mL blood) was chosen depending on previous studies [17]. At the end of the incubation time of each group, the blood was filtered from the microspheres by decantation, and the blood was examined for the following parameters:…”

Section: Resultsmentioning

confidence: 99%

Study of the Effect of Magnetic Microspheres on Some Biophysical Parameters of Human Blood (In vitro Study)

Hereba¹

2012

J Biotechnol Biomaterial

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Magnetic microspheres have many bio-medical applications, but they have contact with the blood before reaching to their target place in many cases, so the study of the effect of these microspheres on the blood can lead to optimization of the use of these microspheres. Five groups of normal blood samples were incubated with 10 mg magnetic chitosan microspheres for 1, 3, 6, 12, and 24 hours (h) respectively, then each group was compared to control group samples. The results showed that increase in hemoglobin conductivity with the time of incubation, no change in red blood cells osmotic fragility, no change in hemoglobin absorbance spectrum. Decrease in blood pH at 24 h, no change in neither hemoglobin electrophoretic patterns or in the blood components ultra structures. These results indicated no obvious toxicity of the magnetic microspheres on blood for incubation time up to 24 h, but on the other hand there are some changes especially at 24 h of incubation, so it is safer to avoid the remaining of the microspheres inside the body for more than 24 h.

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Section: Resultsmentioning

confidence: 99%

Study of the Effect of Magnetic Microspheres on Some Biophysical Parameters of Human Blood (In vitro Study)

Hereba¹

2012

J Biotechnol Biomaterial

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“…Among the suitable degradable polymers is chitosan, which is a potential useful as pharmaceutical material for transdermal patch development owing to its good biocompatibility and low toxicity (16,17). Chitosan is the most abundant basic biopolymer based on a linear amino polysaccharide of D-glucosamine and N-acetyl-D-glucosamine being obtained by the partial deacetylation of natural polymer chitin, the major compound of exoskeletons in crustaceans such as crabs, prawns, lobsters, the cuticles of insects, and the cell walls of fungi (16,18).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the chitosan is soluble after stirring in acids such as acetic, nitric, hydrochloric, perchloric, and phosphoric, but it is insoluble in water, organic solvents, and aqueous bases (19,20). The degradation products of chitosan are nontoxic, nonimmunogenic, and noncarcinogenic making it safe for drug delivery application (17). Recently, chitosan is used to control release and also prepare as hydrogels in transdermal drug delivery systems of many drugs such as repaglinide (8), ciprofloxacin hydrochloride (21), warfarin (22), salicylic acid (23), prednisolone (24), and rhodamine B (25).…”

Section: Introductionmentioning

confidence: 99%

Formulation, Physicochemical Characterization, and In Vitro Study of Chitosan/HPMC Blends-Based Herbal Blended Patches

et al. 2014

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Abstract. The current work prepared chitosan/hydroxypropyl methylcellulose (HPMC) blends and studied the possibility of chitosan/HPMC blended patches for Zingiber cassumunar Roxb. The blended patches without/with crude Z. cassumunar oil were prepared by homogeneously mixing the 3.5% w/v of chitosan solution and 20% w/v of HPMC solution, and glycerine was used as plasticizer. Then, they were poured into Petri dish and produced the blended patches in hot air oven at 70±2°C. The blended patches were tested and evaluated by the physicochemical properties: moisture uptake, swelling ratio, erosion, porosity, Fourier transform infrared spectroscopy, differential scanning calorimetry, and X-ray diffraction, and photographed the surface and cross-section morphology under SEM technique. Herbal blended patches were studied by the in vitro release and skin permeation of active compound D. The blended patches could absorb the moisture and became hydrated patches that occurred during the swelling of blended patches. They were eroded and increased by the number of porous channels to pass through out for active compound D. In addition, the blended patches indicated the compatibility of the blended ingredients and homogeneous smooth and compact. The blended patches made from chitosan/HPMC blends provide a controlled release and skin permeation behavior of compound D. Thus, the blended patches could be suitably used for herbal medicine application.

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“…Another approach is using injectable microbeads, which combine the advantages of high surface areato-volume ratio and more controlled cross-linking efficiency. The use of implant configuration as a basis for producing more potent constructs for tissue engineering and induction of angiogenesis have been inadvertently underestimated and still remain poorly studied for lack of good experimental tools that can continuously and noninvasively monitor the fate of the implants in vivo.Various invasive techniques have been described for documenting implant resorption in vivo; however, these techniques do not provide detailed information about the in situ integration process and require destructive analysis of postmortem sections (13,(16)(17)(18). With the optimization of a hydrogel scaffold requiring more information of the transient steps of integration, techniques to continuously monitor the degradation of a scaffold using fluorescence labeling have been described (15,19,20).…”

mentioning

confidence: 99%

“…Various invasive techniques have been described for documenting implant resorption in vivo; however, these techniques do not provide detailed information about the in situ integration process and require destructive analysis of postmortem sections (13,(16)(17)(18). With the optimization of a hydrogel scaffold requiring more information of the transient steps of integration, techniques to continuously monitor the degradation of a scaffold using fluorescence labeling have been described (15,19,20).…”

mentioning

confidence: 99%

Using bimodal MRI/fluorescence imaging to identify host angiogenic response to implants

Berdichevski

Yameen

Dafni³

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Therapies that promote angiogenesis have been successfully applied using various combinations of proangiogenic factors together with a biodegradable delivery vehicle. In this study we used bimodal noninvasive monitoring to show that the host response to a proangiogenic biomaterial can be drastically affected by the mode of implantation and the surface area-to-volume ratio of the implant material. Fluorescence/MRI probes were covalently conjugated to VEGF-bearing biodegradable PEG-fibrinogen hydrogel implants and used to document the in vivo degradation and liberation of bioactive constituents in an s.c. rat implantation model. The hydrogel biodegradation and angiogenic host response with three types of VEGFbearing implant configurations were compared: preformed cylindrical plugs, preformed injectable microbeads, and hydrogel precursor, injected and polymerized in situ. Although all three were made with identical amounts of precursor constituents, the MRI data revealed that in situ polymerized hydrogels were fully degraded within 2 wk; microbead degradation was more moderate, and plugs degraded significantly more slowly than the other configurations. The presence of hydrogel degradation products containing the fluorescent label in the surrounding tissues revealed a distinct biphasic release profile for each type of implant configuration. The purported in vivo VEGF release profile from the microbeads resulted in highly vascularized s.c. tissue containing up to 16-fold more capillaries in comparison with controls. These findings demonstrate that the configuration of an implant can play an important role not only in the degradation and resorption properties of the materials, but also in consequent host angiogenic response.biomaterial scaffold | angiogenesis | hydrogel | contrast agents | tissue regeneration W ith progress in the field of regenerative medicine relying more on approaches that deliver cells and/or bioactive factors using minimally invasive procedures, functional donor-tohost integration remains a critical challenge. In this context, biodegradable hydrogel scaffolds provide certain advantages, such as excellent tissue compatibility, temporary protection from host inflammation, and controlled resorption based on cell-mediated degradation (1). Effective transport properties to and within an implanted hydrogel are critical in the design of cell-seeded scaffolds, where the delivery of nutrients and removal of waste products from regions deep within the implant can severely limit the use of a patch for only the smallest of grafts (i.e., <0.5 mm) (2-4). Therefore, a great deal of research in the field of regenerative medicine has been devoted to ameliorate the survival of the cells and tissues with implants that use proangiogenic factors.One strategy is to enhance the localized formation of vascularized networks to improve the perfusion of oxygen and nutrients to the intended region in the body (2, 4, 5). This process is regulated by a number of different growth factors that stimulate a complex angiogenic res...

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Optimized preparation of daidzein-loaded chitosan microspheres and in vivo evaluation after intramuscular injection in rats

Cited by 35 publications

References 26 publications

Study of the Effect of Magnetic Microspheres on Some Biophysical Parameters of Human Blood (In vitro Study)

Study of the Effect of Magnetic Microspheres on Some Biophysical Parameters of Human Blood (In vitro Study)

Formulation, Physicochemical Characterization, and In Vitro Study of Chitosan/HPMC Blends-Based Herbal Blended Patches

Using bimodal MRI/fluorescence imaging to identify host angiogenic response to implants

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