Optimized Nitrogen Recovery and Non-Bitter Hydrolysates from Porcine Hemoglobin

Abstract

The combined effects of pH, temperature, buffer/substrate ratio, time and enzyme concentration on nitrogen recovery (NR) from porcine hemoglobin, a by-product of industrial abattoirs, with Pancreatin and Flavourzyme® 500MG enzyme mixture were characterized. The effect of hydrolysis variables on NR was described through response surface analysis (RSA). The results showed that pH and time were the most important parameters and that the buffer/substrate ratio had less of an effect on NR. The mathematical model presented an optimum hydrolysis conditions were as follows: temperature, 50.4 ºC; pH, 7.8; buffer/substrate (containing 33.1% protein) ratio (w/w), 1.4:1; time, 15.4h; and enzyme concentration, 2.0g/kg. The predicted NR value was 98.99%, and the actual value obtained was 97.69%. Molecular mass of recovered hydrolysates ranged from >15 kDa to free amino acid (<1 kDa). The admixture of enzyme had specificity for terminal a variety of hydrophobic amino acids which resulted in recovery of non-bitter hydrolysates from porcine hemoglobin.

Focusing on the Optimization for Scale up in Airlift Bioreactors and the Production of Chlamydomonas reinhardtii as a Model Microorganism

“…Even if not depicted in the text, the normal probability plot versus the internally studentized residuals to check for the normality assumption was also satisfactory with the residuals plot approximated on a straight line. On the other hand, the plot of the internally studentized residuals versus the run order showed the residuals scattered randomly on the graph suggesting that the variance of the variables of the original trials was constant for all the values of both responses (Guo et al 2008, Anbu et al 2009. Response surfaces were generated by plotting responses on the Z-axis against any two variables while keeping the other three as independent variables at their respective '0' levels.…”